Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,613,082	T→C	D57G (GAC→GGC)	glsB ←	glutaminase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,613,082	0	T	C	100.0%	44.3 / NA	14	D57G (GAC→GGC)	glsB	glutaminase 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/10);  total (4/10)

AGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGTAATGACGCATGGCGACAACGAGACTCAGCACTTTGGAAATAGACTGAATGGAAAAACGTTCTTGCGCGTCTCCGGCCTGAAAAAGCTGTCCGTCAACGGTACAGATAGCAATCCCCAATCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCC  >  NC_000913/1612970‑1613183                                                                                                                 |                                                                                                      aGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGTAATGACGCATGGCGACAACGAGACTCAGCACTTTGGAAATAGACTGAATGGAAAAACGTTCTTGCGCGCCTCCGGCCTGAAAAAGCTGTCCGTCa                                                                             <  1:96252/139‑1 (MQ=255)  gACGGATCTTTGCCGACGCGTTGCCAGATTTCCTTTTCGGAGTAATGACGCATGGCGACAACGAGACTCAGCACTTTGGAAATAGACTGAATGGAAAAACGTTCTTGCGCGCCTCCGGCCTGAAAAAGCTGTCCGTCaa                                                                            <  2:201454/139‑1 (MQ=255)   aCGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGTAATGACGCATGGCGACAACGAGACTCAGCACTTTGGAAATAGACTGAATGGAAAAACGTTCTTGCGCGCCTCCGGCCTGAAAAAGCTGTCCGTCAAc                                                                           <  2:73301/139‑1 (MQ=255)     ggATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGTAATGACGCATGGCGACAACGAGACTCAGCACTTTGGAAATAGACTGAATGGAAAAACGTTCTTGCGCGCCTCCGGCCTGAAAAAGCTGTcc                                                                                 <  1:427446/131‑1 (MQ=255)     ggATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGTAATGACGCATGGCGACAACGAGACTCAGCACTTTGGAAATAGACTGAATGGAAAAACGTTCTTGCGCGCCTCCGGCCTGAAAAAGCTGTcc                                                                                 >  2:427446/1‑131 (MQ=255)                 aCGCGTTGCCAGATTTCCTCTTCGGAGTAATGACGCATGGCGACAACGAGACTCAGCACTTGGGAAATAGACTGAATGGGAAAACGGTCTTGCGCGCCTCCGGCCTGAAAAAGCTGTCCGTCAACGGTACAGATAGCaa                                                             >  2:21002/1‑139 (MQ=255)                                                       ggCGACAACGAGACTCAGCACTTTGGAAATAGACTGAATGGAAAAACGTTCTTGCGCGCCTCCGGCCTGAAAAAGCTGTCCGt                                                                               <  1:329194/83‑1 (MQ=255)                                                       ggCGACAACGAGACTCAGCACTTTGGAAATAGACTGAATGGAAAAACGTTCTTGCGCGCCTCCGGCCTGAAAAAGCTGTCCGt                                                                               >  2:329194/1‑83 (MQ=255)                                                        gCGACAACGAGACTCAGCACTTTGGAAATAGACTGAATGGAAAAACGTTCTTGCGCGCCTCCGGCCTGAAAAAGCTGTCCGt                                                                               <  1:344439/82‑1 (MQ=255)                                                        gCGACAACGAGACTCAGCACTTTGGAAATAGACTGAATGGAAAAACGTTCTTGCGCGCCTCCGGCCTGAAAAAGCTGTCCGt                                                                               >  2:344439/1‑82 (MQ=255)                                                                gagaCTCAGCACTTTGGAAATAGACTGAATGGAAAAACGTTCTTGCGCGCCTCCGGCCTGAAAAAGCTGTCCGTCAACGGTACAGATAGCAATCCCCAATCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATcc              <  2:251558/139‑1 (MQ=255)                                                                 agaCTCAGCACTTTGGAAATAGACTGAATGGAAAAACGTTCTTGCGCGCCTCCGGCCTGAAAAAGCTGTCCGTCAACGGTACAGATAGCAATCCCCAATCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCcg             <  1:21002/139‑1 (MQ=255)                                                                 agaCTCAGCACTTTGGAAATAGACTGAATGGAAAAACGTTCTTGCGCGCCTCCGGCCTGAAAAAGCTGTCCGTCAACGGTACAGATAGCAATCCCCAATCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCcg             <  2:436157/139‑1 (MQ=255)                                                                            tttGGAAATAGACTGAATGGAAAAACGTTCTTGCGCGCCTCCGGCCTGAAAAAGCTGTTCGTCAACGGTACAGATAGCAATCCCCAATCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTAccc  <  1:444125/139‑1 (MQ=255)                                                                                                                 |                                                                                                      AGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGTAATGACGCATGGCGACAACGAGACTCAGCACTTTGGAAATAGACTGAATGGAAAAACGTTCTTGCGCGTCTCCGGCCTGAAAAAGCTGTCCGTCAACGGTACAGATAGCAATCCCCAATCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCC  >  NC_000913/1612970‑1613183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑