Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,840,240	G→A	W258* (TGG→TGA)	ynjE →	molybdopterin synthase sulfurtransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,840,240	0	G	A	100.0%	62.2 / NA	19	W258* (TGG→TGA)	ynjE	molybdopterin synthase sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/8);  total (11/8)

ACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGTGACGTATACGCTGCAGCGCGTGTGGCGCAGATTATGCTTTATGCTGGCGTGAAAGATGTGCGCCTGCTGGATGGCGGCTGGCAAACCTGGTCCGACGCGGGACTGCCTGTTGAGCGCGGAACGCCACCGAAAGTGAAAGCGGAACCGGATTTCGGCGTGAAGATCCCGGCACAACCGCAGTTGATGCTCGATATGGAAC  >  NC_000913/1840117‑1840358                                                                                                                            |                                                                                                                       acacGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGTGACGTATACGCTGCAGCGCATGTGGCGCAGATTATGCTTTATGCTGGCGTGAAAGATGTGCGCCTGCTGGATGGCGACTGACAAACCTGGTCCGAc                                                                                                         >  2:298695/1‑139 (MQ=255)                      cacGGTCATTCTGTATGGGCGTGACGTATACGCTGCAGCGCGTGTGGCGCAGATTATGCTTTATGCTGGCGTGAAAGATGTGCGCCTGCTGGATGGCGGCTGACAAACCTGGTCCGACGCGGGACTGCCTGTTGAgcgc                                                                                    >  2:230107/1‑139 (MQ=255)                                       ggCGTGACGTATACGCTGCAGCGCGTGTGGCGCAGATTATGCTTTATGCTGGCGTGAAAGATGTGCGCCTGCTGGATGGCGGCTGACAAACCTGGTCCGACGCGGGACTGCCTGTTGAGCGCGGAACGCCACCGAAAGt                                                                   >  1:363061/1‑139 (MQ=255)                                         cGTGACGTATACGCTGCAGCGCGTGTGGCGCAGATTATGCTTTATGCTGGCGTGAAAGATGTGCGCCTGCTGGATGGCGGCTGACAAACCTGGTCCGACGCGGGACTGCCTGTTGAGCGCGGAACGCCACCGAAAGTGa                                                                 >  2:108423/1‑139 (MQ=255)                                                  taCGCTGCAGCGCGTGTGGCGCAGATTATGCTTTATGCTGGCGTGAAAGATGTGCGCCTGCTGGATGGCGGCTGACAAACCTGGTCCGACGCggg                                                                                                    <  2:264234/95‑1 (MQ=255)                                                  taCGCTGCAGCGCGTGTGGCGCAGATTATGCTTTATGCTGGCGTGAAAGATGTGCGCCTGCTGGATGGCGGCTGACAAACCTGGTCCGACGCggg                                                                                                    >  1:264234/1‑95 (MQ=255)                                                         cAGCGCGTGTGGCGCAGATTATGCTTTATGCTGGCGTGAAAGATGTGCGCCTGCTGGATGGCGGCTGACAAACCTGGTCCGACGCGGGACTGCCTGTTGAGCGCGGAACGCCACCGAAAGTGAAAGCGGAACCGGAttt                                                 >  1:369195/1‑139 (MQ=255)                                                                 gtgGCGCAGATTATGCTTTATGCTGGCGTGAAAGATGTGCGCCTGCTGGATGGCGGCTGACAAACCTGGTcc                                                                                                            >  1:223784/1‑72 (MQ=255)                                                                 gtgGCGCAGATTATGCTTTATGCTGGCGTGAAAGATGTGCGCCTGCTGGATGGCGGCTGACAAACCTGGTcc                                                                                                            <  2:223784/72‑1 (MQ=255)                                                                  tgGCGCAGATTATGCTTTATGCTGGCGTGAAAGATGTGCGCCTGCTGGATGGCGGCTGACAAACCTGGTCCGACGCGGGACTGCCTGTTGAGCGCGGAACGCCACCGAAAGTGAAAGCGGAACCGGATTTCGGCGTGaa                                        <  1:230107/139‑1 (MQ=255)                                                                                    atgctGGCGTGAAAGATGTGCGCCTGCTGGATGGCGGCTGACAAACCTGGTCCGAcg                                                                                                        >  1:330029/1‑57 (MQ=255)                                                                                    atgctGGCGTGAAAGATGTGCGCCTGCTGGATGGCGGCTGACAAACCTGGTCCGAcg                                                                                                        <  2:330029/57‑1 (MQ=255)                                                                                        tGGCGTGAAAGATGTGCGCCTGCTGGATGGCGGCTGACAAACCTGGTCCGACGCGGGACTGCCTGTTGAGCGCGGAACGCCACCGAAATTGAAAGCGGAACCGGATTTCGGCGTGAAGATCCCGGCACAACCGCAGTTg                  >  1:289588/1‑139 (MQ=255)                                                                                          gCGTGAAAGATGTGCGCCTGCTGGATGGCGGCTGACAAACCTGGTCCGACGCGGGACTGCCTGTTGAGCGCGGAACGCCACCGAAAGTGAAAGCGGAACCGGATTTCGGCGTGAAGATCCCGGCACAACCGCAGTTGAt                >  1:51801/1‑139 (MQ=255)                                                                                             tGAAAGATGTGCGCCTGCTGGATGGCGGCTGACAAACCTGGTCCGACGCGGGACTGCCTGTTGAGCGCGGAACGCCACCGAAAGTGAAAGCGGAACCGGATTTCGGCGTGAAGATCCCGGTACAACCGCAGTTGATGCt             <  1:298695/139‑1 (MQ=255)                                                                                                  gATGTGCGCCTGCTGGATGGCGGCTGACAAACCTGGTCCGACGCGGGACTGCCTGTTGAGCGCGGAACGCCACCGAAAGTGAAAGCGGAACCGGATTTCGGCGTGAAGATCCCGGCACAACCGCAGTTGATGCTCGata        <  1:8106/139‑1 (MQ=255)                                                                                                  gATGTGCGCCTGCTGGATGGCGGCTGACAAACCTGGTCCGACGCGGGACTGCCTGTTGAGCGCGGAACGCCACCGAAAGTGAAAGCGGAACCGGATTTCGGCGTGAAGATCCCGGCACAACCGCAGTTGATGCTCGata        <  1:108423/139‑1 (MQ=255)                                                                                                  gATGTGCGCCTGCTGGATGGCGGCTGACAAACCTGGTCCGACGCGGGACTGCCTGTTGAGCGCGGAACGCCACCGAAAGTGAAAGCGGAACCGGATTTCGGCGTGAAGATCCCGGCACAACCGCAGTTGATGCTCGata        <  2:363061/139‑1 (MQ=255)                                                                                                        cgcCTGCTGGATGGCGGCTGACAAACCTGGTCCGACGCGGGACTGCCTGTTGAGCGCGGAACGCCACCGAAAGTGAAAGCGGAACCGGATTTCGGCGTGAAGATCCCGGCACAACCGCAGTTGATGCTCGATATGGAAc  >  1:232395/1‑139 (MQ=255)                                                                                                                            |                                                                                                                       ACACGGCATTCGCCATGACACCACGGTCATTCTGTATGGGCGTGACGTATACGCTGCAGCGCGTGTGGCGCAGATTATGCTTTATGCTGGCGTGAAAGATGTGCGCCTGCTGGATGGCGGCTGGCAAACCTGGTCCGACGCGGGACTGCCTGTTGAGCGCGGAACGCCACCGAAAGTGAAAGCGGAACCGGATTTCGGCGTGAAGATCCCGGCACAACCGCAGTTGATGCTCGATATGGAAC  >  NC_000913/1840117‑1840358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑