Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,907,616	C→T	intergenic (‑25/+645)	yobF ← / ← yebO	DUF2527 family heat‑induced protein/putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,907,616	0	C	T	100.0%	135.2 / NA	40	intergenic (‑25/+645)	yobF/yebO	DUF2527 family heat‑induced protein/putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/18);  total (22/18)

ACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTCTTCCACTTTTTTTGCACACTTATGCAACGGAATACGCGCCGATATATCA  >  NC_000913/1907488‑1907749                                                                                                                                 |                                                                                                                                      aCTGACACATTACTGCATGAGGCACCAATCTAAGGCTCGGCAGAGCAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGt                                                                                                                             >  2:263776/1‑139 (MQ=255)    gACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTAt                                                                                                                          <  1:318824/139‑1 (MQ=255)                cATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGt                                                                                                              >  1:245068/1‑139 (MQ=255)                  tGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTACTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTATACCTCGTTTAACTCGACATCTCTTATCATATACAAcaata                                                                                                            >  2:410203/1‑135 (MQ=255)                     ggCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAAtt                                                                                                         >  2:39118/1‑139 (MQ=255)                     ggCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAAtt                                                                                                         >  1:456269/1‑139 (MQ=255)                          cAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACg                                                                                                               >  1:192772/1‑128 (MQ=255)                          cAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACg                                                                                                               <  2:192772/128‑1 (MQ=255)                                    cTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCAtttt                                                                                          >  2:445700/1‑139 (MQ=255)                                       ggCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTg                                                                                                                              <  2:356773/100‑1 (MQ=255)                                       ggCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTg                                                                                                                              >  1:356773/1‑100 (MQ=255)                                       ggCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTAt                                                                                                                          <  2:277899/104‑1 (MQ=255)                                       ggCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTAt                                                                                                                          >  1:277899/1‑104 (MQ=255)                                       ggCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATa                                                                                                                    <  2:98695/110‑1 (MQ=255)                                       ggCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATa                                                                                                                    >  1:98695/1‑110 (MQ=255)                                       ggCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAAtt                                                                                                         >  1:163821/1‑121 (MQ=255)                                       ggCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAAtt                                                                                                         <  2:163821/121‑1 (MQ=255)                                        gCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTaaa                                                                                      <  2:456269/139‑1 (MQ=255)                                         cAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAAc                                                                                     <  1:263776/139‑1 (MQ=255)                                                                acgtGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATc                                                                                                                         <  2:362029/80‑1 (MQ=255)                                                                acgtGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATc                                                                                                                         >  1:362029/1‑80 (MQ=255)                                                                acgtGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAAt                                                              >  1:60904/1‑139 (MQ=255)                                                                acgtGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAAt                                                              >  1:345018/1‑139 (MQ=255)                                                                     tttACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGa                                                         >  2:140525/1‑139 (MQ=255)                                                                           cAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTc                                                   >  1:326535/1‑139 (MQ=255)                                                                             ggACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTCtt                                                 >  1:166514/1‑139 (MQ=255)                                                                                    tttACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTCTTCCACttt                                          >  2:393792/1‑139 (MQ=255)                                                                                           aaaaTGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTCTTCCACTTTTTTTGca                                   <  1:39118/139‑1 (MQ=255)                                                                                                gCCACACATAAACAGAACTGTACCTCGTTTAACTCGaaa                                                                                                                                  <  1:117511/39‑1 (MQ=255)                                                                                                cccACACATAAACAGAACTGTACCTCGTTTAACTCGaaa                                                                                                                                  >  2:117511/2‑39 (MQ=255)                                                                                                       aTAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTCTTCCACTTTTTTTGCACACTTATGCAAc                       <  2:245068/139‑1 (MQ=255)                                                                                                           aCAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTCTTCCACTTTTTTTGCACACTTATGCAACGGaa                   <  1:393792/139‑1 (MQ=255)                                                                                                                  tGTACCTCTTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTCTTCCACTTTTTTGGCACACTTATGCAACGGAATACgcgc            <  1:368678/139‑1 (MQ=255)                                                                                                                  tGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTCTTCCACTTTTTTTGCACACTTATGCAACGGAATACgcgc            <  1:140525/139‑1 (MQ=255)                                                                                                                    tACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTCTTCCACTTTTTTTGCACACTTATGCAACGGAATACGCGCCg          >  1:329019/1‑139 (MQ=255)                                                                                                                    tACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTCTTCCACTTTTTTTGCACACTTATGCAACGGAATACGCGCCg          >  1:405977/1‑139 (MQ=255)                                                                                                                         cGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTCTTCCACTTTTTTTGCACACTTATGCAACGGAATAcgcg             >  1:151178/1‑131 (MQ=255)                                                                                                                         cGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTCTTCCACTTTTTTTGCACACTTATGCAACGGAATAcgcg             <  2:151178/131‑1 (MQ=255)                                                                                                                            ttAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTCTTCCACTTTTTTTGCACACTTATGCAACGGAATACGCGCCGATATATCa  <  2:198943/139‑1 (MQ=255)                                                                                                                              aaCTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTCTTCCACTTTTTTTGCACACTTATGCAACGGAATACgcgc            <  1:375869/127‑1 (MQ=255)                                                                                                                                 |                                                                                                                                      ACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTCTTCCACTTTTTTTGCACACTTATGCAACGGAATACGCGCCGATATATCA  >  NC_000913/1907488‑1907749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑