| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,910,043 | A→C | V19V (GTT→GTG) | kdgR ← | KDG regulon transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,910,043 | 0 | A | C | 92.0% | 84.4 / 0.6 | 25 | V19V (GTT→GTG) | kdgR | KDG regulon transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base C (12/11); total (13/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.40e-01 | |||||||||||
CATAACCTAAGGTTTTCATGGTCTGTAAAAAGCGATAAACGGTGCTTTTTGACATCATGACGCGCTGCGACAGCTCGGTTATCCCTATTTCGCGCTCTTCACCCAGCGCCTGCAAAATGCCAAAAACTTTTAGCACGGAAGATACAGAATCAGGCTGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAAT > NC_000913/1909919‑1910175 | caTAACCTAAGGTTTTCATGGTCTGTAAAAAGCGATAAACGGTGCTTTTTGACATCATGACGCGCTGCGACAGCTCGGTTATCCCTATTTCGCGCTCTTCACCCAGCGCCTGCAAAATGCCAAAAACTTTTAGCACGGa > 2:239122/1‑139 (MQ=255) ccTAAGGTTTTCATGGTCTGTAAAAAGCGATAAACGGTGCTTTTTGACATCATGACGCGCTGCGACAGCTCGGTTATCCCTATTTCGCGCTCTTCACCCAGCGCCTGCAAAATGCCAAACACTTTTAGCACGGAAGATa > 2:261011/1‑139 (MQ=255) tCATGGTCTGTAAAAAGCGATAAACGGTGCTTTTTGACATCATGACGCGCTGCGACAGCTCGGTTATCCCTATTTCGCGCTCTTCACCCAGCGCCTGCAAAATGCCAAAAACTTTTAGCACGGAAGATACAGAATCAgg < 1:239122/139‑1 (MQ=255) gTAAAAAGCGATAAACGGTGCTTTTTGACATCATGACGCGCTGCGACAGCTCGGTTATCCCTATTTCGCGCTCTTCACCCAGCGCCTGCAAAATGCCAAACACTTTTAGCACGGAAGATACAGAATCAGGCTGTTTATc < 1:365457/139‑1 (MQ=255) aaGCGATAAACGGTGCTTTTTGACATCATGACGCGCTGCGACAGCTCGGTTATCCCTATTTCGCGCTCTTCACCCAGCGCCTGCAAAATGCCAAACACTTTTAGCACGGAAGATACAGAATCAGGCTGTTTATCCAGAt < 1:261011/139‑1 (MQ=255) tAAACGGTGCTTTTTGACATCATGACGCGCTGCGACAGCTCGGTTATCCCTATTTCGCGCTCTTCACCCAGCGCCTGCAAAATGCCAAACACTTTTAGCACGGAAGATACAGAATCAg < 1:69403/118‑1 (MQ=255) tAAACGGTGCTTTTTGACATCATGACGCGCTGCGACAGCTCGGTTATCCCTATTTCGCGCTCTTCACCCAGCGCCTGCAAAATGCCAAACACTTTTAGCACGGAAGATACAGAATCAg > 2:69403/1‑118 (MQ=255) tcatGACGCGCTGCGACAGCTCGGTTATCCCTATTTCGCGCTCTTCACCCAGCGCCTGCAAAATGCCAAACACTTTTAGCACGGAAGATACAGAATCAGGCTGt < 1:135538/104‑1 (MQ=255) tcatGACGCGCTGCGACAGCTCGGTTATCCCTATTTCGCGCTCTTCACCCAGCGCCTGCAAAATGCCAAACACTTTTAGCACGGAAGATACAGAATCAGGCTGt > 2:135538/1‑104 (MQ=255) atGACGCGCTGCGACAGCTCGGTTATCCCTATTTCGCGCTCTTCACCCAGCGCCTGCAAAATGCCAAACACTTTTAGCACGGAAGATACAGAATCAGGCTGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTg > 1:299678/1‑139 (MQ=255) gcTGCGACAGCTCGGTTATCCCTATTTCGCGCTCTTCACCCAGCGCCTGCAAAATGCCAAACACTTTTAGCACGGAAGATACAGAATCAGGCTGt < 1:340423/95‑1 (MQ=255) gcTGCGACAGCTCGGTTATCCCTATTTCGCGCTCTTCACCCAGCGCCTGCAAAATGCCAAACACTTTTAGCACGGAAGATACAGAATCAGGCTGt > 2:340423/1‑95 (MQ=255) tGCGACAGCTCGGTTATCCCTATTTCGCGCTCTTCACCCAGCGCCTGCAAAATGCCAAACACTTTTAGCACGGAAGATACAGAATCAg < 1:429853/88‑1 (MQ=255) tGCGACAGCTCGGTTATCCCTATTTCGCGCTCTTCACCCAGCGCCTGCAAAATGCCAAACACTTTTAGCACGGAAGATACAGAATCAg > 2:429853/1‑88 (MQ=255) cTCGGTTATCCCTATTTCGCGCTCTTCACCCAGCGCCTGCAAAATGCCAAACACTTTTAGCACGGAAGATACAGAATCAGGCTGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATaaaaa > 2:367307/1‑139 (MQ=255) aTTTCGCGCTCTTCACCCAGCGCCTGCAAAATGCCAAACACTTTTAGCACGGAAGATACAGAATCAGGCTGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGtttt > 2:119232/1‑139 (MQ=255) cgcTCTTCACCCAGCGCCTGCAAAATGCCAAACACTTTTAGCa > 2:297604/1‑43 (MQ=255) cgcTCTTCACCCAGCGCCTGCAAAATGCCAAACACTTTTAGCa < 1:297604/43‑1 (MQ=255) cgcTCTTCACCCAGCGCCTGCAAAATGCCAAACACTTTTAGCACGGAAGATACAGAATCAg > 2:158154/1‑61 (MQ=255) cgcTCTTCACCCAGCGCCTGCAAAATGCCAAACACTTTTAGCACGGAAGATACAGAATCAg < 1:158154/61‑1 (MQ=255) cgcTCTTCACCCAGCGCCTGCAAAATGCCAAACACTTTTAGCACGGAAGATACAGAATCAGGCTGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTttatta > 2:214575/1‑139 (MQ=255) gcTCTTCACCCAGCGCCTGCAAAATGCCAAACACTTTTAGCACGGAAGATACAGAATCAGGCTGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTtattat < 1:214575/139‑1 (MQ=255) gCAAAATGCCAAACACTTTTAGCACGGAAGATACAGAATCAGGCTGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGggtgg > 2:330927/1‑139 (MQ=255) gCCAAACACTTTTAGCACGGAAGATACAGAATCAGGCTGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAAt < 1:119232/139‑1 (MQ=255) gCCAAACACTTTTAGCACGGAAGATACAGAATCAGGCTGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAAt < 1:416664/139‑1 (MQ=255) | CATAACCTAAGGTTTTCATGGTCTGTAAAAAGCGATAAACGGTGCTTTTTGACATCATGACGCGCTGCGACAGCTCGGTTATCCCTATTTCGCGCTCTTCACCCAGCGCCTGCAAAATGCCAAAAACTTTTAGCACGGAAGATACAGAATCAGGCTGTTTATCCAGATCTGCGTTAGCCATTTATCACCTCATTGCGAGTGTTTTATAAAAATCAGAACTGTTTTTTATTATAATTTCGCACCAGGGTGGTCGCAAT > NC_000913/1909919‑1910175 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |