Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,457,127	A→T	M678K (ATG→AAG)	fadJ ←	enoyl‑CoA hydratase/epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,457,127	0	A	T	100.0%	47.6 / NA	15	M678K (ATG→AAG)	fadJ	enoyl‑CoA hydratase/epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (8/7);  total (8/7)

TGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAA  >  NC_000913/2457007‑2457259                                                                                                                         |                                                                                                                                     tGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCTTTATTGCAACCACTTCGc                                                                                                                    >  2:317924/1‑139 (MQ=255)  gACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCTTTATTGCAACCACTTCGcc                                                                                                                   <  1:288366/139‑1 (MQ=255)                  gTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCTTTATTGCa                                                                                                                              >  1:179972/1‑112 (MQ=255)                  gTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCTTTATTGCa                                                                                                                              <  2:179972/112‑1 (MQ=255)                  gTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCTTTATTGCAACCACTTCGCCCGCGCCgag                                                                                                          <  1:408763/132‑1 (MQ=255)                  gTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCTTTATTGCAACCACTTCGCCCGCGCCgag                                                                                                          >  2:408763/1‑132 (MQ=255)                            gttgttTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCTTTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGa                                                                                         >  1:268330/1‑139 (MQ=255)                               gttTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCTTTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACg                                                                                      >  1:433793/1‑139 (MQ=255)                                                         gcCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCTTTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCa                                                            <  2:433793/139‑1 (MQ=255)                                                                       aaCGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCTTTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACcgc                                              >  1:414823/1‑139 (MQ=255)                                                                            tCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCTTTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCa                                                            <  1:175344/120‑1 (MQ=255)                                                                            tCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCTTTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCa                                                            >  2:175344/1‑120 (MQ=255)                                                                                                      cTGCGTGGCAAGTCGTTGCTTTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATa               <  1:317924/139‑1 (MQ=255)                                                                                                           tGGCAAGTCGTTGCTTTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTg          >  2:409997/1‑139 (MQ=255)                                                                                                                   cGTTGCTTTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCaa  <  2:414823/139‑1 (MQ=255)                                                                                                                         |                                                                                                                                     TGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAA  >  NC_000913/2457007‑2457259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑