Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,823,930	G→A	S139S (AGC→AGT)	pncC ←	nicotinamide‑nucleotide amidohydrolase, NMN amidohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,823,930	0	G	A	100.0%	178.5 / NA	52	S139S (AGC→AGT)	pncC	nicotinamide‑nucleotide amidohydrolase, NMN amidohydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/24);  total (28/24)

AATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGACACGGCATAATCAGCAC  >  NC_000913/2823806‑2824063                                                                                                                             |                                                                                                                                      aaTATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCccg                                                                                                                         <  1:76012/139‑1 (MQ=255)         cTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTaa                                                                                                                 >  2:37474/1‑139 (MQ=255)                      tataCTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACcacg                                                                                                    >  2:376857/1‑139 (MQ=255)                               tGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGc                                                                                           >  2:199376/1‑139 (MQ=255)                               tGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGc                                                                                           <  2:89772/139‑1 (MQ=255)                                    aTACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAg                                                                                      >  1:219583/1‑139 (MQ=255)                                     tACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCAtt                                                                                                                             >  2:237086/1‑99 (MQ=255)                                     tACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCAtt                                                                                                                             <  1:237086/99‑1 (MQ=255)                                     tACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGt                                                                                                                   <  2:374391/109‑1 (MQ=255)                                     tACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGt                                                                                                                   >  1:374391/1‑109 (MQ=255)                                         gTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCt                                                                                                            <  2:54272/112‑1 (MQ=255)                                         gTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCt                                                                                                            >  1:54272/1‑112 (MQ=255)                                         gTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAAc                                                                                 >  1:365614/1‑139 (MQ=255)                                          tATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAAcc                                                                                >  2:391390/1‑139 (MQ=255)                                           aTCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCa                                                                               >  1:175692/1‑139 (MQ=255)                                            tCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAg                                                                              >  2:370732/1‑139 (MQ=255)                                            tCAAGTGCTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAg                                                                              >  2:440670/1‑139 (MQ=255)                                             cAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGa                                                                             <  1:37474/139‑1 (MQ=255)                                             cAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGa                                                                             <  1:391390/139‑1 (MQ=255)                                             cAAGTGCTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGa                                                                             <  1:440670/139‑1 (MQ=255)                                                      tGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCAt                                                                                                                              >  2:100332/1‑81 (MQ=255)                                                      tGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCAt                                                                                                                              <  1:100332/81‑1 (MQ=255)                                                                              tGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAg                                                                              <  1:174539/105‑1 (MQ=255)                                                                              tGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAg                                                                              >  2:174539/1‑105 (MQ=255)                                                                                      aTACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCa                                                                                    >  1:106170/1‑91 (MQ=255)                                                                                      aTACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATccggcccg                                    >  1:75227/1‑139 (MQ=255)                                                                                      aTACGCAGTAGCCTGACGACGCACCGCATCACGGGCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCa                                                                                    <  2:106170/91‑1 (MQ=255)                                                                                       tACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATccggcccgg                                   <  2:75227/139‑1 (MQ=255)                                                                                        aCGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATccggcccggc                                  >  1:266500/1‑139 (MQ=255)                                                                                          gCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGa                                >  1:350725/1‑139 (MQ=255)                                                                                          gCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGa                                <  2:219583/139‑1 (MQ=255)                                                                                           cAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGAt                               <  2:175692/139‑1 (MQ=255)                                                                                           cAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGAt                               <  1:370732/139‑1 (MQ=255)                                                                                            aGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGc                                                                                           <  1:223837/78‑1 (MQ=255)                                                                                            aGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGc                                                                                           >  2:223837/1‑78 (MQ=255)                                                                                             gTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATAc                             >  2:332312/1‑139 (MQ=255)                                                                                             gTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATAc                             <  1:199376/139‑1 (MQ=255)                                                                                             gTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATAc                             >  1:353811/1‑139 (MQ=255)                                                                                             gTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATAc                             >  1:360615/1‑139 (MQ=255)                                                                                              tAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACa                                                                  <  1:404527/101‑1 (MQ=255)                                                                                              tAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACa                                                                  >  2:404527/1‑101 (MQ=255)                                                                                              tAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAgg                                                                <  2:58383/103‑1 (MQ=255)                                                                                              tAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAgg                                                                >  1:58383/1‑103 (MQ=255)                                                                                               aGCCTTCCGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAATCCAGTCGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCa                           <  2:139283/139‑1 (MQ=255)                                                                                               aGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGa                                                                             <  1:448938/89‑1 (MQ=255)                                                                                               aGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGa                                                                             >  2:448938/1‑89 (MQ=255)                                                                                               aGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCa                           >  1:9029/1‑139 (MQ=255)                                                                                                 ccTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAgg                                                                >  2:253365/1‑100 (MQ=255)                                                                                                 ccTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAgg                                                                <  1:253365/100‑1 (MQ=255)                                                                                                       gacgCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAACAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGa                   <  2:266500/139‑1 (MQ=255)                                                                                                       gacgCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCACTGGCAAAAGCAAACCAGCCGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGa                   <  2:365614/139‑1 (MQ=255)                                                                                                             ccGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGACACGGc             <  1:332312/139‑1 (MQ=255)                                                                                                                        tCGCCACTGAAGCATTCCCGCCGGCTAATGCCTTCACCGCGGGCAGTAGTAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCACTCCAGTCGATACCACTAATATACACGGCAAAATCAGCAc  >  2:125483/1‑139 (MQ=255)                                                                                                                             |                                                                                                                                      AATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGACACGGCATAATCAGCAC  >  NC_000913/2823806‑2824063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑