Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	276,574	T→C	V417A (GTG→GCG)	mmuP →	CP4‑6 prophage, putative S‑methylmethionine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	276,574	0	T	C	100.0%	57.0 / NA	16	V417A (GTG→GCG)	mmuP	CP4‑6 prophage, putative S‑methylmethionine transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/9);  total (7/9)

CGTCATCTGCAACAAGGTAAGGCATTGAGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAGTATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATCCAGCGCAGAGAATTGCGTTGTGGTGCGGGTTACCGTTTGTTGCGTTGTGCTATGGTGCTTATTTCCTTACT  >  NC_000913/276492‑276683                                                                                   |                                                                                                              cGTCATCTGCAACAAGGTAAGGCATTGAGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAGTATTAGGTTTTGCGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATCCAGCGCAGAGAATTGCGt                                                       <  2:93188/139‑1 (MQ=255)                           gAGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAGTATTAGGTTTTGCGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATCCAGCGCAGAGAATTGCGTTGTGGTGCGGGTTACCGTttgtt                                <  2:394280/136‑1 (MQ=255)                           gAGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAGTATTAGGTTTTGCGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATCCAGCGCAGAGAATTGCGTTGTGGTGCGGGTTACCGTttgtt                                >  1:394280/1‑136 (MQ=255)                           gAGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAGTATTAGGTTTTGCGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATCCAGCGCAGAGAATTGCGTTGTGGTGCGGGTTACCGTTTGTTGCg                             <  1:337857/139‑1 (MQ=255)                            aGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAGTATTAGGTTTtgcgct                                                                                                            <  1:71218/59‑1 (MQ=255)                            aGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAGTATTAGGTTTtgcgct                                                                                                            >  2:71218/1‑59 (MQ=255)                            aGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAGTATTAGGTTTTGCGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATCCAGCGCAGAGAATTGCGTTGTGGTGCGGGTTACCGTttgtt                                <  1:332093/135‑1 (MQ=255)                            aGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAGTATTAGGTTTTGCGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATCCAGCGCAGAGAATTGCGTTGTGGTGCGGGTTACCGTttgtt                                >  2:332093/1‑135 (MQ=255)                              tGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAGTATTAGGTTTTGCGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATCCAGCGCAGAGAATTGCGTTGTGGTGCGGGTTACCGTTTGTTGCGTtg                          <  1:50812/139‑1 (MQ=255)                                          tCGCGCGCCGTGGTATCCGCTGGTGCCAGTATTAGGTTTTGCGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATCCAGCGCAGAGAATTGCGTTGTGGTGCGGGTTAcc                                       >  1:106624/1‑114 (MQ=255)                                          tCGCGCGCCGTGGTATCCGCTGGTGCCAGTATTAGGTTTTGCGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATCCAGCGCAGAGAATTGCGTTGTGGTGCGGGTTAcc                                       <  2:106624/114‑1 (MQ=255)                                             cgcgcCGTGGTATCCGCTGGTGCCAGTATTAGGTTTTGCGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCAtt                                                                              >  2:283890/1‑72 (MQ=255)                                             cgcgcCGTGGTATCCGCTGGTGCCAGTATTAGGTTTTGCGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATCCAGCGCAGAGAATTGCGTTGTGGTGCGGGTTACCGTTTGTTGCGTTGTGCTATGGTGCTTAt           >  1:329707/1‑139 (MQ=255)                                             cgcacCGTGGTATCCGCTGGTGCCAGTATTAGGTTTTGCGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCAtt                                                                              <  1:283890/72‑1 (MQ=255)                                                    tGGTATCCGCTGGTGCCAGTATTAGGTTTTGCGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATCCAGCGCAGAGAATTGCGTTGTGGTGCGGGTTACCGTTTGTTGCGTTGTGCTATGGTGCTTATTTCCTTa    >  1:188060/1‑139 (MQ=255)                                                      gTATCCGCTGGTGCCAGTATTAGGTTTTGCGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATCCAGCGCAGAGAATTGCGTTGTGGTGCGGGTTACCGTTTGTTGCGTTGTGCTATGGTGCTTATTTCCTTACt  <  2:188060/139‑1 (MQ=255)                                                              tGGTGCCAGTATTAGGTTTTGCGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATCCAGCGCAGAGAATTGCGTTGTGGTGCGGGTTACCGTTTGTTGCGTTGTGCTATgg                  <  1:398376/115‑1 (MQ=255)                                                              tGGTGCCAGTATTAGGTTTTGCGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATCCAGCGCAGAGAATTGCGTTGTGGTGCGGGTTACCGTTTGTTGCGTTGTGCTATgg                  >  2:398376/1‑115 (MQ=255)                                                                                   |                                                                                                              CGTCATCTGCAACAAGGTAAGGCATTGAGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAGTATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATCCAGCGCAGAGAATTGCGTTGTGGTGCGGGTTACCGTTTGTTGCGTTGTGCTATGGTGCTTATTTCCTTACT  >  NC_000913/276492‑276683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑