Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,873,023	T→C	L105L (CTA→CTG)	ygbE ←	DUF3561 family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,873,023	0	T	C	100.0%	100.0 / NA	27	L105L (CTA→CTG)	ygbE	DUF3561 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/13);  total (14/13)

ATACCGCGACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGTACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTGTAGCGTAGCTTTCCGTCCATCAATGAATGCAGCGCAATCCCCACCACTACCGCGACGGGCATCAGCGCCAGAA  >  NC_000913/2872888‑2873156                                                                                                                                        |                                                                                                                                      aTACCGCGACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGTACAAATTAGCCCagcagc                                                                                                                                    <  1:43186/139‑1 (MQ=255)     cgcgACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGTACAAATTAGCCCAGCAGCcaca                                                                                                                                >  1:269037/1‑139 (MQ=255)     cgcgACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGTACAAATTAGCCCAGCAGCcaca                                                                                                                                >  2:329775/1‑139 (MQ=255)        gACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGTACAAATTAGCCCAGCAGCCACATaa                                                                                                                             >  1:90038/1‑139 (MQ=255)                        gTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGTACAAATTAGCCCAGCAGCCACATAAACAGTGCGCCAAACAt                                                                                                             <  1:171237/139‑1 (MQ=255)                           ccACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAAGATAATCTCAGTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGTACAAATTAGCCCAGCAGCCACATAAACAGTGCGCCAAACATAAt                                                                                                          >  2:280746/1‑139 (MQ=255)                             acaTTTGTCGATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGTACAAATTAGCCCAGCAGCCACATAAACAGTGCGCCAAACATAATGc                                                                                                        <  2:269037/139‑1 (MQ=255)                               aTTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGTACAAATTAGCCCAGCAGCCACATAAACAGTGCGCCAAACATAATGCCt                                                                                                      <  1:400738/139‑1 (MQ=255)                                             aCGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACATAGGAGACGGGAAAGTACAAATTAGCCCAGCAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAgggc                                                                                        <  1:280746/139‑2 (MQ=255)                                                   aCCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGTACAAATTAGCCCAGCAGCCACATAAACAGTGCGCCAAACATAATGACTACTGTCACCAGGGTGAAAAc                                                                                  >  1:21771/1‑139 (MQ=255)                                                         ggCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGTACAAATTAGCCCAGCAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGt                                                                                        <  2:439846/127‑1 (MQ=255)                                                         ggCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGTACAAATTAGCCCAGCAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGt                                                                                        >  1:439846/1‑127 (MQ=255)                                                             tGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGTACAAATTAGCCCAGCAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGaaaa                                                                                   <  2:443865/128‑1 (MQ=255)                                                             tGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGTACAAATTAGCCCAGCAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGaaaa                                                                                   >  1:443865/1‑128 (MQ=255)                                                             tGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGTACAAATTAGCCCAGCAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTgtag                                                                        >  2:34565/1‑139 (MQ=255)                                                             tGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGTACAAATTAGCCCAGCAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTgtag                                                                        >  1:394113/1‑139 (MQ=255)                                                                  aaCATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGTACAAATTAGCCCAGCAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTgtagcgtag                                                                   <  2:90038/139‑1 (MQ=255)                                                                    cATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGTACAAATTAGCCCAGCAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTGTAGCGTAGCt                                                                 >  2:365024/1‑139 (MQ=255)                                                                             attattAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGTACAAATTAGCCCAGCAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTGTAGCGTAGCTTTCCGTCCa                                                        <  1:365024/139‑1 (MQ=255)                                                                             attattAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGTACAAATTAGCCCAGCAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTGTAGCGTAGCTTTCCGTCCa                                                        <  1:34565/139‑1 (MQ=255)                                                                              ttattaGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGTACAAATTAGCCCAGCAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTgtagcgtagc                                                                  <  1:367867/128‑1 (MQ=255)                                                                              ttattaGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGTACAAATTAGCCCAGCAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTgtagcgtagc                                                                  >  2:323589/1‑128 (MQ=255)                                                                              ttattaGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGTACAAATTAGCCCAGCAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTgtagcgtagc                                                                  >  2:367867/1‑128 (MQ=255)                                                                              ttattaGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGTACAAATTAGCCCAGCAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTgtagcgtagc                                                                  <  1:323589/128‑1 (MQ=255)                                                                                       aCGGTATCAATTATGAACAGAGGAGACGAGAAAGAAAAAATTAGCCAAGCAGCCACATAAAAAGAACGCCAAACAAAATGCCTAATGTTACCAGGGTGAAAACAACACTTTAGCGTAGCTTTCCGTCCATCaatgaatg                                              <  2:81149/139‑1 (MQ=255)                                                                                              gAATTATGAACAGAGGAGACGGGAAAGTACAAATTAGCCCAGCAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTGTAGCGTAGCTTTCCGTCCATCAATGAATGCAGCGCa                                       <  2:240072/139‑1 (MQ=255)                                                                                               aaTTATGAACAGAGGAGACGGGAAAGTACAAATTAGCCCAGCAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTGTAGCGTAGCTTTCCGTCCATCAATGAATGCAGCGCaa                                      >  1:217205/1‑139 (MQ=255)                                                                                                                                   cccAGCAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTGTAGCGTAGCTTTCCGTCCATCAATGAATGCAGCGCAATCCCCACCACTACCGCGACGGGCATCAGCGCCagaa  >  1:298988/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                      ATACCGCGACAACGCGGGCAAATGTACCACATTTGTCCATTGTTACGTATACCCAGGGCGTGCAGAACATAATCTCATTATTAGTTACGGTTTGAATTATGAACAGAGGAGACGGGAAAGTACAAATTAGCCCAGTAGCCACATAAACAGTGCGCCAAACATAATGCCTACTGTCACCAGGGTGAAAACAATACTGTAGCGTAGCTTTCCGTCCATCAATGAATGCAGCGCAATCCCCACCACTACCGCGACGGGCATCAGCGCCAGAA  >  NC_000913/2872888‑2873156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑