Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,256,941	G→A	A357V (GCC→GTC)	yhaO ←	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,256,941	0	G	A	100.0%	66.2 / NA	20	A357V (GCC→GTC)	yhaO	putative transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/11);  total (9/11)

CCCTACCATGCCGAAAATCGGGCTACAGATAGAGGTGAAGCTCAACACCGGTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGGCAGGCATCTTGCGACGCAGGATGTTCATTACGATCCCTTGCGTTGCTTCGCGAAAGCCTAAATAGACGCCAAAGAATGCAGTCATGACAGCAAAGATATTGAGAATGACGCTGA  >  NC_000913/3256805‑3257055                                                                                                                                         |                                                                                                                   cccTACCATGCCGAAAATCGGGCTACAGATAGAGGTGAAGCTCACCACCGGTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCgaca                                                                                                                  >  2:323175/1‑139 (MQ=255)         tGCCGAAAATCGGGCTACAGATAGAGGTGAAGCTCAACACCGGTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCtt                                                                                                          >  1:150266/1‑139 (MQ=255)          gCCGAAAATCGGGCTACAGATAGAGGTGAAGCTCAACACCGGTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTg                                                                                                         <  1:323175/139‑1 (MQ=255)                       cTACAGATAGAGGTGAAGCTCAACACCGGTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGt                                                                                            <  2:322652/139‑1 (MQ=255)                                  ggTGAAGCTCAACACCGGTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTACGATc                                                                                 >  1:184470/1‑139 (MQ=255)                                    tGAAGCTCAACACCGGTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCtt                                                                                                          <  1:402415/112‑1 (MQ=255)                                    tGAAGCTCAACACCGGTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCtt                                                                                                          >  2:402415/1‑112 (MQ=255)                                    tGAAGCTCAACACCGGTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTc                                                                                          >  1:365093/1‑128 (MQ=255)                                    tGAAGCTCAACACCGGTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTc                                                                                          <  2:365093/128‑1 (MQ=255)                                            aaCACCGGTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTACGATCCCTTGCGTTg                                                                       >  1:363555/1‑139 (MQ=255)                                                   gTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGACGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTACGATCCCTTGCGTTGCTTcgcg                                                                >  1:74279/1‑139 (MQ=255)                                                     gCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTACGATCCCTTGCGTTGCTTCGCGaa                                                              <  2:320916/139‑1 (MQ=255)                                                        ttCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTAc                                                                                     <  2:91576/113‑1 (MQ=255)                                                        ttCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTAc                                                                                     >  1:91576/1‑113 (MQ=255)                                                        ttCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTACGATCCCTTGCGTTGCTTCGCGAAAGc                                                           <  2:363555/139‑1 (MQ=255)                                                                gATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTACGATCCCTTGCGTTGCTTCGCGAAAGCCTAAATAg                                                   <  2:150266/139‑1 (MQ=255)                                                                                  cAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTACGATCCCTTGCGTTGCTTCGCGaa                                                              >  2:238755/1‑110 (MQ=255)                                                                                  cAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTACGATCCCTTGCGTTGCTTCGCGaa                                                              <  1:238755/110‑1 (MQ=255)                                                                                              aaTCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTACGATCCCTTGCGTTGCTTCGCGAAAGCCTAAATAGACGCCAAAGAATGCAGTCATGACAGCAAAg                     <  2:184470/139‑1 (MQ=255)                                                                                                         cgcgCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTACGATCCCTTGCGTTGCTTCGCGAAAGCCTAAATAGACGCCAAAGAATGCAGTCATGACAGCAAAGATATTGAGAAt          >  2:404949/1‑139 (MQ=255)                                                                                                                 aCGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTACGATCCCTTGCGTTGCTTCGCGAAAGCCTAAATAGACGCCAAAGAATGCAGTCATGACAGCAAAGATATTGAGAATGACGCTGa  <  2:74279/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                   CCCTACCATGCCGAAAATCGGGCTACAGATAGAGGTGAAGCTCAACACCGGTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGGCAGGCATCTTGCGACGCAGGATGTTCATTACGATCCCTTGCGTTGCTTCGCGAAAGCCTAAATAGACGCCAAAGAATGCAGTCATGACAGCAAAGATATTGAGAATGACGCTGA  >  NC_000913/3256805‑3257055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑