Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,315,163	G→A	G284G (GGC→GGT)	infB ←	translation initiation factor IF‑2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,315,163	0	G	A	100.0%	34.5 / NA	13	G284G (GGC→GGT)	infB	translation initiation factor IF‑2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/7);  total (6/7)

GTTCGCCAACGGTGATAGTTTCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAGCCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCG  >  NC_000913/3315054‑3315286                                                                                                              |                                                                                                                            gttcgccaACGGTGATAGTTTCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAGCCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCACCACGTACTGCTGCGCGTGCTTCTTCACg                                                                                                >  1:146212/1‑139 (MQ=255)    cgccaACGGTGATAGTTTCGCCGATCACAACGTCACGGTTATCGGCCTGAGCAGGCTTCTGGAAGCCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCACCACGTACTGCTGCGCGTGCTTCTTCACGATc                                                                                             >  2:281306/1‑139 (MQ=255)               aTAGTTTCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAGCCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCACCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGAtt                                                                                  <  2:146212/139‑1 (MQ=255)                     tCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAGCCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCACCACGTACTGCTGCGCGTg                                                                                                          <  1:399272/109‑1 (MQ=255)                     tCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAGCCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCACCACGTACTGCTGCGCGTg                                                                                                          >  2:399272/1‑109 (MQ=255)                     tCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAGCCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCACCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCgt                                                                            >  1:141362/1‑139 (MQ=255)                        ccGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAGCCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCACCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGtgtt                                                                         <  2:280461/139‑1 (MQ=255)                                             aCGGCCTGATCAGGCTTCTGTAAGCCTTGCTGCAGCGAAGATCCTTTACGTTTTCCGCCTTTACCACCTCGTACTGTTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGAcgcg                                                    <  1:106071/139‑1 (MQ=255)                                                               tGGAAGCCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCACCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGAc                                  >  1:207250/1‑139 (MQ=255)                                                                ggAAGCCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCACCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGAcc                                 <  2:141362/139‑1 (MQ=255)                                                                                     aaCCTTTACGTTTTCCGCCTTTACCACCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTa                                      >  1:421911/1‑113 (MQ=255)                                                                                     aaCCTTTACGTTTTCCGCCTTTACCACCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTa                                      <  2:421911/113‑1 (MQ=255)                                                                                               ttttCCGCCTTTACCACCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCg  <  2:446514/139‑1 (MQ=255)                                                                                                              |                                                                                                                            GTTCGCCAACGGTGATAGTTTCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAGCCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCG  >  NC_000913/3315054‑3315286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑