Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,946,742	C→T	noncoding (43/120 nt)	rrfC →	5S ribosomal RNA of rrnC operon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,946,742	0	C	T	100.0%	36.1 / NA	17	noncoding (43/120 nt)	rrfC	5S ribosomal RNA of rrnC operon
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/11);  total (6/11)

ACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCA  >  NC_000913/3946608‑3946818                                                                                                                                       |                                                                             aCAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCTGAAc                                                                          >  2:107762/1‑139 (MQ=37)       ccGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCTGAACTCAGaa                                                                    >  2:378807/1‑139 (MQ=25)             aTGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCTGAACTCAGAAGTGaaa                                                              <  1:179458/139‑1 (MQ=18)                    ggCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCTGAACTCAGAAGTGAAACGCCGtc                                                       <  2:285373/139‑2 (MQ=17)                        gATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCTGAACTCAGAAGTGAAACGCCGTAgcgc                                                   <  1:378807/139‑1 (MQ=18)                           gagagaAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCTGAACTCAGAAGTGAAACGCCGTAGCGCCGa                                                <  1:9624/139‑1 (MQ=18)                           gagagaAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCTGAACTCAGAAGTGAAACGCCGTAGCGCCGa                                                <  1:157751/139‑1 (MQ=17)                                 agaTTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCTGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAg                                          <  1:19248/139‑1 (MQ=17)                                   aTTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCCGTCGCGCGGTGGTCCCACCTGACCCCATGCTGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAgtg                                        >  2:370733/1‑139 (MQ=17)                                             tGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCTGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTcc                              <  1:310665/139‑1 (MQ=18)                                                     aTTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCTGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGt                                  >  1:318243/1‑127 (MQ=17)                                                     aTTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCTGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGt                                  <  2:318243/127‑1 (MQ=18)                                                               aaCGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCTGAACTCAGAAGTGAAACGCCGTAGCGCCGATgg                                             <  2:211048/106‑1 (MQ=18)                                                               aaCGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCTGAACTCAGAAGTGAAACGCCGTAGCGCCGATgg                                             >  1:211048/1‑106 (MQ=18)                                                                 cGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCTGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGc                        <  1:38685/125‑1 (MQ=18)                                                                 cGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCTGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGc                        >  2:38685/1‑125 (MQ=18)                                                                         cGGTCTGATGAAACAGAATTTGCCTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCTGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCa  <  2:374568/139‑1 (MQ=17)                                                                                                                                       |                                                                             ACAACGCCGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCA  >  NC_000913/3946608‑3946818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑