| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,969,883 | A→T | N285Y (AAT→TAT) | wzzE → | Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,969,883 | 0 | A | T | 94.7% | 59.5 / ‑3.1 | 19 | N285Y (AAT→TAT) | wzzE | Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base T (11/7); total (11/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01 | |||||||||||
CGCAGTGCGACAGATGTACCTGCCGAGGAATTACCTGATTCAGAAATGTTCCTGCTTGGGCGTCCAATGCTTCAGGCTCGACTGGAAAATTTACAGGCCGTCGGTCCGGCCTTTGATCTCGACTATGATCAGAATCGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTG > NC_000913/3969751‑3970016 | cgcAGTGCGACAGATGTACCTGCCGAGGAATTACCTGATTCAGAAATGTTCCTGCTTGGGCGTCCAATGCTTCAGGCTCGACTGGAAAATTTACAGGCCGTCGGTCCGGCCTTTGATCTCGACTATGATCAGTATCggg < 1:406014/139‑1 (MQ=255) gACAGATGTACCTGCCGAGGAATTACCTGATTCAGAAATGTTCCTGCTTGGGCGTCCAATGCTTCAGGCTCGACTGGAAAATTTACAGGCCGTCGGTCCGGCCTTTGATCTCGACTATGATCAGAATCGGGCCATGTTa < 2:229424/139‑1 (MQ=255) aCCTGCCGAGGAATTACCTGATTCAGAAATGTTCCTGCTTGGGCGTCCAATGCTTCAGGCTCGACTGGAAAATTTACAGGCCGTCGGTCCGGCCTTTGATCTCGACTATGATCAGTATCGGGCCATGTTAAACACCCTg > 2:88915/1‑139 (MQ=255) gCCGAGGAATTACCTGATTCAGAAATGTTCCTGCTTGGGCGTCCAATGCTTCAGGCTCGACTGGAAAATTTACAGGCCGTCGGTCCGGCCTTTGATCTCGACTATGATCAGTATCGGGCCATGTTAAACACCCTGAATg > 2:356297/1‑139 (MQ=255) ccGAGGAATTACCTGATTCAGAAATGTTCCTGCTTGGGCGTCCAATGCTTCAGGCTCGACTGGAAAATTTACAGGCCGTCGGTCCGGCCTTTGATCTCGACTATGATCAGTATCGGGCCATGTTAAac < 1:108436/128‑1 (MQ=255) ccGAGGAATTACCTGATTCAGAAATGTTCCTGCTTGGGCGTCCAATGCTTCAGGCTCGACTGGAAAATTTACAGGCCGTCGGTCCGGCCTTTGATCTCGACTATGATCAGTATCGGGCCATGTTAAac > 2:108436/1‑128 (MQ=255) cGAGGAATTACCTGATTCAGAAATGTTCCTGCTTGGGCGTCCAATGCTTCAGGCTCGACTGGAAAATTTACAGGCCGTCGGTCCGGCCTTTGATCTCGACTATGATCAGTATCGGGCCATGTTAAACACCCTGAATGtt > 1:143881/1‑139 (MQ=255) aaTTACCTGATTCAGAAATGTTCCTGCTTGGGCGTCCAATGCTTCAGGCTCGACTGGAAAATTTACAGGCCGTCGGTCCGGCCTTTGATCTCGACTATGATCAGTATCGGGCCATGTTAAACACCCTGAATGTTGGTcc < 1:88915/139‑1 (MQ=255) ggCGTCCAATGCTTCAGGCTCGACTGGAAAATTTACAGGCCGTCGGTCCGGCCTTTGATCTCGACTATGATCAGTATCGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCg > 2:202976/1‑139 (MQ=255) gCGTCCAATGCTTCAGGCTCGACTGGAAAATTTACAGGCCGTCGGTCCGGCCTTTGATCTCGACTATGATCAGTATCGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGc < 2:143881/139‑1 (MQ=255) cAATGCTTCAGGCTCGACTGGAAAATTTACAGGCCGTCGGTCCGGCCTTTGATCTCGACTATGATCAGTATCGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTAttt > 2:330739/1‑139 (MQ=255) cAGGCTCGACTGGAAAATTTACAGGCCGTCGGTCCGGCCTTTGATCTCGACTATGATCAGTATCGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGc > 1:401936/1‑139 (MQ=255) cAGGCTCGACTGGAAAATTTACAGGCCGTCGGTCCGGCCTTTGATCTCGACTATGATCAGTATCGGGCCATGTTAAACACCCTCAATGTTGGTCCAACCCTGGATCCGCGTCTTCAGACCTATCGCTATTTGCGTACGc > 2:18462/1‑139 (MQ=255) aGGCTCGACTGGAAAATTTACAGGCCGTCGGTCCGGCCTTTGATCTCGACTATGATCAGTATCGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGcc > 2:416001/1‑139 (MQ=255) cGACTGGAAAATTTACAGGCCGTCGGTCCGGCCTTTGATCTCGACTATGATCAGTATCGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGgaag < 1:330739/139‑1 (MQ=255) tGATCTCGACTATGATCAGTATCGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAAcgcg < 1:416505/119‑1 (MQ=255) tGATCTCGACTATGATCAGTATCGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAAcgcg > 2:416505/1‑119 (MQ=255) aTCTCGACTATGATCAGTATCGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCt < 1:202976/139‑1 (MQ=255) aTCAGTATCGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTAtgtg > 2:137284/1‑139 (MQ=255) | CGCAGTGCGACAGATGTACCTGCCGAGGAATTACCTGATTCAGAAATGTTCCTGCTTGGGCGTCCAATGCTTCAGGCTCGACTGGAAAATTTACAGGCCGTCGGTCCGGCCTTTGATCTCGACTATGATCAGAATCGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTG > NC_000913/3969751‑3970016 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |