| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,160,278 | G→A | S171S (AGC→AGT) | sthA ← | pyridine nucleotide transhydrogenase, soluble |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,160,278 | 0 | G | A | 100.0% | 83.9 / NA | 25 | S171S (AGC→AGT) | sthA | pyridine nucleotide transhydrogenase, soluble |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (13/12); total (13/12) | |||||||||||
ATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGTTAGTGTTTCAACGCTGCCGTCCG > NC_000913/4160143‑4160402 | aTCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTg > 1:354313/1‑139 (MQ=255) gCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGt < 1:124113/139‑1 (MQ=255) ccAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTc < 2:221658/139‑1 (MQ=255) cagGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTg < 2:82291/139‑1 (MQ=255) tCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTg < 2:354313/139‑1 (MQ=255) aTACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTc > 1:254248/1‑95 (MQ=255) aTACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTc < 2:254248/95‑1 (MQ=255) aTACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGgat > 2:111010/1‑139 (MQ=255) aTACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGgat > 2:371099/1‑139 (MQ=255) aTACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGgat > 1:201030/1‑139 (MQ=255) ccGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTGGCTGTCGTAAATGCGTGGATGGGTGAAATCAAc > 1:245719/1‑98 (MQ=255) ccGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAAc < 2:245719/98‑1 (MQ=255) aTCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGc < 1:242168/64‑1 (MQ=255) aTCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGc > 2:242168/1‑64 (MQ=255) aTCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGga < 1:449429/105‑1 (MQ=255) aTCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGga > 2:449429/1‑105 (MQ=255) aTCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAAtt > 2:248039/1‑139 (MQ=255) cATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGTTAGTGTTTCAACGCt < 1:248039/139‑1 (MQ=255) ggCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGga < 1:394214/77‑1 (MQ=255) ggCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGga > 2:394214/1‑77 (MQ=255) cgcgGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATc < 2:163383/62‑1 (MQ=255) cgcgGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATc > 1:163383/1‑62 (MQ=255) cgGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGga > 2:21081/1‑73 (MQ=255) cgGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGga < 1:21081/73‑1 (MQ=255) ggTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGTTAGTGTTTCAACGCTGCCGTCCg > 2:193036/1‑139 (MQ=255) | ATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGTTAGTGTTTCAACGCTGCCGTCCG > NC_000913/4160143‑4160402 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |