Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,385,906	T→C	L68P (CTC→CCC)	yjeO →	inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,385,906	0	T	C	100.0%	52.3 / NA	15	L68P (CTC→CCC)	yjeO	inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/8);  total (7/8)

ATGATTGATGGACGCGGGATTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCTCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCAGTTT  >  NC_000913/4385794‑4386009                                                                                                                 |                                                                                                        atgatTGATGGACGCGGGATTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGAAAAAAAc                                                                               >  1:245523/1‑139 (MQ=255) atgatTGATGGACGCGGGATTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGAAAAAAAc                                                                               >  2:373930/1‑139 (MQ=255)    attgatGGACGCGGGATTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGAAAAAAAcgcg                                                                            <  2:392004/139‑1 (MQ=255)     ttgatGGACGCGGGATTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGAAAAAAACGCgg                                                                           <  1:373930/139‑1 (MQ=255)     ttgatGGACGCGGGATTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGAAAAAAACGCgg                                                                           <  2:73488/139‑1 (MQ=255)        atGGACGCGGGATTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGAAAAAAACGCGGc                                                                          >  1:356155/1‑137 (MQ=255)        atGGACGCGGGATTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGAAAAAAACGCGGc                                                                          <  2:356155/137‑1 (MQ=255)                              cTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGAAAAAAAcgcg                                                                            >  1:203392/1‑113 (MQ=255)                              cTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGAAAAAAAcgcg                                                                            <  2:203392/113‑1 (MQ=255)                                gCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGTTTATGTACGCCACCGCGCTGGc                                                >  1:95620/1‑139 (MQ=255)                                                tctcGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGTTTATGTACGCCACcgcg                                                     >  1:243363/1‑118 (MQ=255)                                                tctcGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGTTTATGTACGCCACcgcg                                                     <  2:243363/118‑1 (MQ=255)                                                     aagaagACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCtgtg                           <  1:289440/139‑1 (MQ=255)                                                                         gTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATc       <  2:95620/139‑1 (MQ=255)                                                                              tgtgATAATGACGTTACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCAGttt  >  1:428062/1‑139 (MQ=255)                                                                                                                 |                                                                                                        ATGATTGATGGACGCGGGATTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCTCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCAGTTT  >  NC_000913/4385794‑4386009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑