Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,490,935	A→G	L69L (TTG→CTG)	idnR ←	transcriptional repressor, 5‑gluconate‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,490,935	0	A	G	100.0%	71.5 / NA	20	L69L (TTG→CTG)	idnR	transcriptional repressor, 5‑gluconate‑binding
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/11);  total (9/11)

ACCGACTCTTCTTCGGAATCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAAGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATTTTGGCGATGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGA  >  NC_000913/4490805‑4491058                                                                                                                                   |                                                                                                                            aCCGACTCTTCTTCGGAATCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGa                                                                                                                     <  1:120663/139‑1 (MQ=255)    gACTCTTCTTCGGAATCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGa                                                                                                                     <  1:201740/136‑1 (MQ=255)    gACTCTTCTTCGGAATCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGa                                                                                                                     >  2:201740/1‑136 (MQ=255)    gACTCTTCTTCGGAATCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAggg                                                                                                                  >  1:234737/1‑139 (MQ=255)                gAATCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTtgtg                                                                                                      <  1:77559/139‑1 (MQ=255)                   tCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCAt                                                                                                   >  1:178740/1‑139 (MQ=255)                    cgcgATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCAtt                                                                                                  <  2:234737/139‑1 (MQ=255)                                     ttAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAg                                                                                 <  1:11886/139‑1 (MQ=255)                                                 gTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATGTTGAAATGACGGAATCAGGATACCGAGGGTATCACTTTGTGCATTCAACAACATGACGGAAGCGCGCTTAGGGa                                                                     >  2:369722/1‑139 (MQ=255)                                                   cTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGata                                                                   >  2:15599/1‑139 (MQ=255)                                                             tGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTtgtg                                                                                                      >  2:434924/1‑94 (MQ=255)                                                             tGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTtgtg                                                                                                      <  1:434924/94‑1 (MQ=255)                                                                    agGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATCTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCAt                                                  >  1:166223/1‑139 (MQ=255)                                                                              ttCTACTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAATTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATTTTGGCg                                        <  1:283761/139‑1 (MQ=255)                                                                                                    gCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATTTTGGCGATGCGCTCGCCTGTTTCCTTTg                  <  1:15599/139‑1 (MQ=255)                                                                                                                    gAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATTTTGGCGATGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGa  <  2:178740/139‑1 (MQ=255)                                                                                                                      aaTGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAg                                                                                 <  1:390958/58‑1 (MQ=255)                                                                                                                      aaTGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAg                                                                                 >  2:390958/1‑58 (MQ=255)                                                                                                                          aCGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAg                                                                        <  2:435512/63‑1 (MQ=255)                                                                                                                          aCGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAg                                                                        >  1:435512/1‑63 (MQ=255)                                                                                                                                   |                                                                                                                            ACCGACTCTTCTTCGGAATCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAAGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATTTTGGCGATGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGA  >  NC_000913/4490805‑4491058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑