Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,351,196	+A	intergenic (‑157/+211)	fabI ← / ← ycjD	enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,351,196	1	.	A	100.0%	54.6 / NA	18	intergenic (‑157/+211)	fabI/ycjD	enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (9/9);  total (9/9)

TTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGA  >  NC_000913/1351067‑1351328                                                                                                                                   |                                                                                                                                     ttgtGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGACGCTTTTc                                                                                                                              >  1:195186/1‑139 (MQ=255)   gtgCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGACGCTTTTccc                                                                                                                            <  2:71365/139‑1 (MQ=255)      ccTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGACGCTTTTCCCCTc                                                                                                                         >  1:417350/1‑139 (MQ=255)                                 aGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGACGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa                                                                                              <  2:417350/139‑1 (MQ=255)                                                             attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGACGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTAc                                                                  >  2:230261/1‑139 (MQ=255)                                                                        ccTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGACGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGcaacaa                                                       >  2:495622/1‑139 (MQ=255)                                                                         cTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGACGCTTTTCCCCTCCCCCTAACCCTCTCCCCa                                                                                                       >  2:38598/1‑90 (MQ=255)                                                                         cTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGACGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa                                                      <  1:495622/139‑1 (MQ=255)                                                                         cTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGACGCTTTTCCCCTCACCCTAACCCGCTCCCCa                                                                                                       <  1:38598/90‑1 (MQ=39)                                                                                  aGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGACGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATtgt                                                                                <  2:14505/104‑1 (MQ=255)                                                                                  aGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGACGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATtgt                                                                                >  1:14505/1‑104 (MQ=255)                                                                                          taGTTGCCAGGTCCGACCGAAGCAGGCTGCGGCAGGGGGGGACGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGcccc                                     >  2:334003/1‑139 (MQ=255)                                                                                               gCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGACGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa                                                                                              <  1:464059/77‑1 (MQ=255)                                                                                               gCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGACGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa                                                                                              >  2:464059/1‑77 (MQ=255)                                                                                                                   gCTGCGGCAGGGGGGGACGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTggg            <  1:334003/139‑1 (MQ=255)                                                                                                                    cTGCGGCAGGGGGGGACGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa           <  1:230261/139‑1 (MQ=255)                                                                                                                        ggCAGGGGGGGACGCTTTTCCCCTCACCCTAAccc                                                                                                               <  2:342175/35‑1 (MQ=18)                                                                                                                        ggCAGGGGGGGACGCTTTTCCCCTCACCCTAAccc                                                                                                               >  1:342175/1‑35 (MQ=18)                                                                                                                            gggggga‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGa  >  1:429621/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                                     TTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGA  >  NC_000913/1351067‑1351328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑