Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,271,600	(C)5→6	coding (171/1146 nt)	garK ←	glycerate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,271,595	1	.	C	100.0%	29.8 / NA	9	A59G (GCT→GGT)	garK	glycerate kinase I
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (4/5);  total (4/5)

CTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAG‑CCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCCCGA  >  NC_000913/3271490‑3271690                                                                                                           |                                                                                                cTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCAcc                                                                 >  2:237513/1‑139 (MQ=255)                                tGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAatca                                                                            >  1:17473/1‑97 (MQ=255)                                tGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAatca                                                                            <  2:17473/97‑1 (MQ=255)                                        ccGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTAc                          <  1:237513/139‑1 (MQ=255)                                                          gCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGaa                                <  1:232579/115‑1 (MQ=255)                                                          gCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGaa                                >  2:232579/1‑115 (MQ=255)                                                          gCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAG‑c                                                                                                <  1:228652/50‑1 (MQ=255)                                                          gCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAG‑c                                                                                                >  2:228652/1‑50 (MQ=255)                                                                aCTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCCCGa  <  2:9520/139‑1 (MQ=255)                                                                          ccAGCGGCCCTGTAACCCAGGCGTGACGTTCAG‑cccccctg                                                                                         <  1:237249/41‑4 (MQ=255)                                                                          ccAGCGGCCCTGTAACCCAGGCGTGACGTTCAG‑cccccctg                                                                                         >  2:237249/1‑38 (MQ=255)                                                                                                   aCGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTa                           <  1:478577/79‑1 (MQ=255)                                                                                                   aCGTTCAGCCCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTa                           >  2:478577/1‑79 (MQ=255)                                                                                                           |                                                                                                CTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAG‑CCCCCTGGGTGGCTGCAATCATCGCTTCCACCGTTCCTTCGCCACCGTCGGCAACCGGAACAGAAACGTACTGTGCATCAGGAAAAATTTCCCGA  >  NC_000913/3271490‑3271690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑