| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,328,816 | (A)7→8 | intergenic (‑101/‑147) | greA ← / → dacB | transcript cleavage factor/D‑alanyl‑D‑alanine carboxypeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,328,809 | 1 | . | A | 80.0% | 35.3 / 4.9 | 15 | intergenic (‑94/‑154) | greA/dacB | transcript cleavage factor/D‑alanyl‑D‑alanine carboxypeptidase |
| Reads supporting (aligned to +/- strand): ref base . (2/1); new base A (5/7); total (7/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01 | |||||||||||
GTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAAA‑TACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACTTTATAGGGCGTTGCGCCGTAGTATGACGGCT > NC_000913/3328718‑3328936 | gTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGCAAAAAAAA‑TACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTca < 2:356513/139‑1 (MQ=255) ctTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAAA‑TACCGACCCGGGTACAAGTCCCAGGTCAg > 1:217079/1‑119 (MQ=255) ctTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAAA‑TACCGACCCGGGTACAAGTCCCAGGTCAg < 2:217079/119‑1 (MQ=255) ttGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTG‑CCGGCATAACTCCTTACCGGGACCAGAAGCAAAAAAAA‑TACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAg > 2:46119/1‑139 (MQ=255) tCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGCAAAAAAAA‑TACCGAc > 1:446560/1‑89 (MQ=255) tCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGCAAAAAAAA‑TACCGAc < 2:446560/89‑1 (MQ=255) cacccacaCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAAAGTACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAgg > 1:328557/1‑139 (MQ=255) cATAACTCCTTACCGGGACCAGAAGCAAAAAAAA‑TACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACTTTATAGGGCGTTgcgc < 1:465469/139‑1 (MQ=255) tAACTCCTTACCGGGACCAGAAGCAAAAAAAA‑TACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACTTTATAGGGCGTTGCGCCg < 1:46119/139‑1 (MQ=255) ggACCAGAAGCAAAAAAAA‑TACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACTTTATAGGGGGTTGCGCCGTAGTATGACGGCt > 1:257395/1‑139 (MQ=255) ggACCAGAAGCAAAAAAAA‑TACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACTTTATAGGGGGTTGCGCCGTAGTATGACGGCt < 2:257395/139‑1 (MQ=255) gACCAGAAGCAAAAAAAA‑TACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACTTTATAGGGCGTTgcgc > 1:300669/1‑123 (MQ=255) gACCAGAAGCAAAAAAAA‑TACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACTTTATAGGGCGTTgcgc < 2:300669/123‑1 (MQ=255) gAAGCAAAAAAAA‑TACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACTTTATAGGGCGTTGCGcc > 1:225825/1‑119 (MQ=255) gAAGCAAAAAAAA‑TACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACTTTATAGGGCGTTGCGcc < 2:225825/119‑1 (MQ=255) | GTTATACCTCTTGAATATTCCTGATAGGGCAAGTCTTCACCCACCCCGGCTGTTCGGCCTGCCCGGCATAACTCCTTACCGGGACCAGAAGC‑AAAAAAA‑TACCGACCCGGGTACAAGTCCCAGGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACTTTATAGGGCGTTGCGCCGTAGTATGACGGCT > NC_000913/3328718‑3328936 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |