| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 506,758 | (T)6→7 | coding (325/480 nt) | ybaK ← | Cys‑tRNA(Pro)/Cys‑tRNA(Cys) deacylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 506,752 | 1 | . | T | 100.0% | 20.5 / NA | 8 | R111S (CGT→AGT) | ybaK | Cys‑tRNA(Pro)/Cys‑tRNA(Cys) deacylase |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (4/4); total (4/4) | |||||||||||
TGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACG‑TTTTTTCTGCCCCAGTGGGCTAATCCCCCCAACCAGGTATCCCGTCGAACGCTGCGCGACCATCGGATCGGCCATCTCAACTTTCTTGGCACCCAGCGCTTTTGCTACTTTTTTAAGA > NC_000913/506660‑506870 | tGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACGTTTTTTTCTGCCCCAGTGGGCTAATCCCCCCAACCAGGTATCCCGt > 1:185861/1‑139 (MQ=255) gccAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACG‑tttttttctg < 1:21430/99‑5 (MQ=255) gccAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACG‑tttttttctg > 2:21430/1‑95 (MQ=255) gcTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACGTTTTTTTCTGCCCCAGTGGGCTAATCCCCCCAACCAGGTATCCCGTCGAACGCTGCGCGACCatcggatcgg < 2:185861/139‑1 (MQ=255) gCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACGTTTTTTTCTGCCCCAGTGGGCTAATCCCCCCAACCAGGTATCCCGTCGAACGCTGCGCGACCATCGGATCGGCCATCTCAACTTTCTTGGCACCCAgc > 1:236118/1‑139 (MQ=255) cTTGTGCGGGGGCGTCGATAATCGTTGGCAGACGTTTTTTTCTGCCCCAGTGGGCTAATCCCCCCTACCATGTATCCCGTCGAACGCTGCGCGACCATCGGCTCGGCCATCTCTACTTTCTTGGCACCCAGCGCTTTTg < 1:133170/139‑1 (MQ=255) ggggCGTCGATAATCGTTGGCAGACGTTTTTTTCTGCCCCAGTGGGCTAATCCCCCCAACCAGGTATCCCGTCGAACGCTGCGCGACCATCGGATCGGCCATCTCAACTTTCTTGGCACCCAGCGCTTTTGCTACtttt > 1:12087/1‑139 (MQ=255) tCGATAATCGTTGGCAGACGTTTTTTTCTGCCCCAGTGGGCTAATCCCCCCAACCAGGTATCCCGTCGAACGCTGCGCGACCATCGGATCGGCCATCTCAACTTTCTTGGCACCCAGCGCTTTTGCTACTTTTTTAAGa < 2:236118/139‑1 (MQ=255) tCGTTGGCAGACGTTTTTTTCTGCCCCAGTGGGCTAATCCCCCCAACCAGGTATCCCGTCGAACGCTgcg < 1:341130/70‑1 (MQ=255) tCGTTGGCAGACGTTTTTTTCTGCCCCAGTGGGCTAATCCCCCCAACCAGGTATCCCGTCGAACGCTgcg > 2:341130/1‑70 (MQ=255) | TGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCGATAATCGTTGGCAGACG‑TTTTTTCTGCCCCAGTGGGCTAATCCCCCCAACCAGGTATCCCGTCGAACGCTGCGCGACCATCGGATCGGCCATCTCAACTTTCTTGGCACCCAGCGCTTTTGCTACTTTTTTAAGA > NC_000913/506660‑506870 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |