Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,415,887 (G)6→7 coding (1613/1626 nt) aidB → DNA alkylation damage repair protein, flavin‑containing DNA binding protein, weak isovaleryl CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,415,8811.G100.0% 99.6 / NA 28T536R (ACG→AGG) aidBDNA alkylation damage repair protein, flavin‑containing DNA binding protein, weak isovaleryl CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (15/13);  total (15/13)

TGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAGATGA  >  NC_000913/4415746‑4416013
                                                                                                                                        |                                                                                                                                    
tgctgGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACggg                                                                                                                                   >  1:376346/1‑139 (MQ=255)
 gctgGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACgggg                                                                                                                                  <  2:286226/139‑1 (MQ=255)
            gTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAgtgtgtg                                                                                                                        >  1:334324/1‑139 (MQ=255)
              gcgcAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAgtgtgtgtg                                                                                                                      <  2:386399/139‑1 (MQ=255)
                  aaaTGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAg                                                                                                                  <  2:996/139‑1 (MQ=255)
                               tGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACgggggggagtg                                                                                                                           <  1:202870/116‑6 (MQ=255)
                               tGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACgggggggagtg                                                                                                                           >  2:202870/1‑111 (MQ=255)
                                 cTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGAc                                                                                                                                       <  1:365791/103‑1 (MQ=255)
                                 cTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGAc                                                                                                                                       >  2:365791/1‑103 (MQ=255)
                                    ctcCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGa                                                                                                >  1:215816/1‑139 (MQ=255)
                                    ctcCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGa                                                                                                >  2:378365/1‑139 (MQ=255)
                                                       gtggtgTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTa                                                                             <  2:376346/139‑1 (MQ=255)
                                                               aGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAat                                                                     >  2:378497/1‑139 (MQ=255)
                                                                    aTGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGaaa                                                                      <  2:47246/133‑1 (MQ=255)
                                                                    aTGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATAAGACTGATGCGACGCTGGTTTCGATTAACTAAATGaaa                                                                      >  1:47246/1‑133 (MQ=255)
                                                                     tGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTaa                                                                                >  1:359495/1‑122 (MQ=255)
                                                                     tGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTaa                                                                                <  2:359495/122‑1 (MQ=255)
                                                                              cgcgcgGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAg                                                      >  1:96149/1‑139 (MQ=255)
                                                                                                  tCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCgcgc                                  >  1:164954/1‑139 (MQ=255)
                                                                                                   cAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGcgcc                                 <  2:215816/139‑1 (MQ=255)
                                                                                                           aTCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTaa                                                                                <  2:195435/84‑1 (MQ=255)
                                                                                                           aTCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTaa                                                                                >  1:195435/1‑84 (MQ=255)
                                                                                                           aTCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCgg                         >  1:453911/1‑139 (MQ=255)
                                                                                                                  aTGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGtt                  >  1:237745/1‑139 (MQ=255)
                                                                                                                     aTTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGa                                                                                    <  1:91895/70‑1 (MQ=255)
                                                                                                                     aTTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGa                                                                                    >  2:91895/1‑70 (MQ=255)
                                                                                                                     aTTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCAt               <  2:334324/139‑1 (MQ=255)
                                                                                                                           tgctgcGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCAt               >  1:466344/1‑133 (MQ=255)
                                                                                                                           tgctgcGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCAt               <  2:466344/133‑1 (MQ=255)
                                                                                                                               gcGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCg                                                  <  1:387661/94‑1 (MQ=255)
                                                                                                                               gcGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCg                                                  >  2:387661/1‑94 (MQ=255)
                                                                                                                               gcGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTGTCGATTAACTAAATGAAATATGGGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAGa     >  1:184922/1‑139 (MQ=255)
                                                                                                                                  ggCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAGATGa  <  1:378497/139‑1 (MQ=255)
                                                                                                                                  ggCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAGATGa  <  1:378365/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                    
TGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGTAGATGA  >  NC_000913/4415746‑4416013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: