Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913566,2770CT41.9% 28.3 / 47.6 43pseudogene (100/279 nt)exoDpseudogene, DLP12 prophage, phage‑type exonuclease family,Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base C (12/13);  new base T (9/9);  total (21/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCA  >  NC_000913/566140‑566414
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tGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCt                                                                                                                                          >  2:92461/1‑139 (MQ=255)
           ttttttCGATGAACTCCGGCACGATCTCGTCAAAACTCGCCATGTACTTTTCATCCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCAc                                                                                                                               <  2:464046/139‑1 (MQ=255)
             ttttCGCTGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTGCATTCCGCTCAATCACCACATAATGCAGGCCCTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCAccc                                                                                                                             <  1:254613/139‑1 (MQ=255)
             ttttCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCAccc                                                                                                                             <  1:239534/139‑1 (MQ=255)
                tCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCcaca                                                                                                                          <  1:402304/139‑1 (MQ=255)
                  gATGAACTCCGGCACGATCTCGTCAAAACTCGCCATGTACTTTTCATCCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCCACATg                                                                                                                        <  2:379777/139‑1 (MQ=255)
                        cTCCGGCACGATCTCGTCAAAACTCGCCATGTACTTTTCATCCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCCACATGCTGTAc                                                                                                                  <  1:285179/139‑1 (MQ=255)
                                   tctcGTCAAAACTCGCCATGTACTTTTCATCCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCcaca                                                                                                                          >  2:221579/1‑120 (MQ=255)
                                   tctcGTCAAAACTCGCCATGTACTTTTCATCCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCcaca                                                                                                                          <  1:221579/120‑1 (MQ=255)
                                                             tCATCCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCTGACTTTATGGCCTCGa                                                                             <  1:166445/139‑1 (MQ=255)
                                                               aTTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTAt                                                                                     >  2:252443/1‑129 (MQ=255)
                                                               aTTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTAt                                                                                     <  1:252443/129‑1 (MQ=255)
                                                                 cccGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCTGACTTTATGGCCTCGAAacc                                                                         >  2:121215/2‑139 (MQ=255)
                                                                   cGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCTGACTTTATGGCCTCGAAaccac                                                                       <  1:121215/139‑1 (MQ=255)
                                                                    gCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCAccc                                                                                                                             <  1:317602/84‑1 (MQ=255)
                                                                    gCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCAccc                                                                                                                             >  2:317602/1‑84 (MQ=255)
                                                                    gCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCcac                                                                                                                           >  2:267034/1‑86 (MQ=255)
                                                                    gCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCcac                                                                                                                           <  1:267034/86‑1 (MQ=255)
                                                                     cTCAATCACGACATAATGCAGGCCTTCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCcac                                                                                                                           <  1:126677/85‑1 (MQ=255)
                                                                     cTCAATCACGACATAATGCAGGCCTTCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCcac                                                                                                                           >  2:126677/1‑85 (MQ=255)
                                                                              gACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCcac                                                                                                                           >  1:309865/1‑76 (MQ=255)
                                                                              gACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCcac                                                                                                                           <  2:309865/76‑1 (MQ=255)
                                                                                  tAATGCAGGCCTTCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCTGACTTTATGGCCTCGAAACCACCGAGCCGGAACTTCa                                                        >  1:138454/1‑139 (MQ=255)
                                                                                  tAATGCAGGCCTTCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCTGACTTTATGGCCTCGAAACCACCGAGCCGGAACTTCa                                                        >  1:144675/1‑139 (MQ=255)
                                                                                    aTGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATg                                                      <  1:92461/139‑1 (MQ=255)
                                                                                     tGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGc                                                                                  >  2:500115/1‑110 (MQ=255)
                                                                                     tGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGc                                                                                  <  1:500115/110‑1 (MQ=255)
                                                                                               cACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCTGACTTTATGGCCTCGAAACCACCGAGCCGGAACTTCATGAAATCCCGGGa                                           >  2:404864/1‑139 (MQ=255)
                                                                                                    ttCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTAc                                      >  2:29665/1‑139 (MQ=255)
                                                                                                          cgcgGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTAt                                <  1:29665/139‑1 (MQ=255)
                                                                                                              ggTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCTGACTTTATGGCCTCGAAACCACCGAGCCGGAACTTCATGAAATCCCGGGAGGTaaacgggcattt                            >  2:330893/1‑128 (MQ=255)
                                                                                                              ggTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCAccc                                                                                                                             <  1:121036/42‑1 (MQ=255)
                                                                                                              ggTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCAccc                                                                                                                             >  2:121036/1‑42 (MQ=255)
                                                                                                              ggTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGcc                            >  1:151277/1‑139 (MQ=255)
                                                                                                              ggTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGcc                            >  1:462794/1‑139 (MQ=255)
                                                                                                                  aTAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACt                                                                                                                 <  2:269646/50‑1 (MQ=255)
                                                                                                                  aTAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACt                                                                                                                 >  1:269646/1‑50 (MQ=255)
                                                                                                                         gCAAAGTACCAGGCATTTTTTCGCGTCACCCACATg                                                                                                                        >  2:171344/1‑36 (MQ=255)
                                                                                                                         gCAAAGTACCAGGCATTTTTTCGCGTCACCCACATg                                                                                                                        <  1:171344/36‑1 (MQ=255)
                                                                                                                               tACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTAt           >  2:87275/1‑139 (MQ=255)
                                                                                                                                aCCAGGCATTTTTTCGCGTCACCCACATGCTGTACTg                                                                                                                >  1:500381/1‑37 (MQ=255)
                                                                                                                                aCCAGGCATTTTTTCGCGTCACCCACATGCTGTACTg                                                                                                                <  2:500381/37‑1 (MQ=255)
                                                                                                                                        tCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCa  <  2:204739/139‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                         
TGCCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCA  >  NC_000913/566140‑566414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑