Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	28,072	C→T	G260G (GGC→GGT)	rihC →	ribonucleoside hydrolase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	28,072	0	C	T	100.0%	58.2 / NA	19	G260G (GGC→GGT)	rihC	ribonucleoside hydrolase 3
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/9);  total (10/9)

CCACTACCGTAGCGGCAGTATGCAAAGCGGCTTGCGAATGCACGATCTCTGCGCCATCGCCTGGCTGGTGCGCCCGGACCTGTTCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGCGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAA  >  NC_000913/27949‑28207                                                                                                                            |                                                                                                                                        ccACTACCGTAGCGGCAGTATGCAAAGCGGCTTGCGAATGCACGATCTCTGCGCCATCGCCTGGCTGGTGCGCCCGGACCTGTTCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGc                                                                                                                          <  1:228206/139‑1 (MQ=255)           aGCGGCAGTATGCAAAGCGGCTTGCGAATGCACGATCTCTGCGCCATCGCCTGGCTGGTGCGCCCGGACCTGTTCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCACGACggtgg                                                                                                                >  1:37586/1‑139 (MQ=255)                            cGGCTTGCGAATGCACGATCTCTGCGCCATCGCCTGGCTGGTGCGCCCGGACCTGTTCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGc                                                                                               <  2:192893/139‑1 (MQ=255)                              gCTTGCGAATGCACGATCTCTGCGCCATCGCCTGGCTGGTGCGCCCGGACCTGTTCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGCCt                                                                                             >  1:431326/1‑139 (MQ=255)                                         cACGATCTCTGCGCCATCGCCTGGCTGGTGCGCCCGGACCTGTTCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAagccag                                                                                  >  1:157108/1‑139 (MQ=255)                                                 ctGCGCCATCGCCTGGCTGGTGCGCCCGGACCTGTTCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTa                                                                          >  2:333542/1‑139 (MQ=255)                                                      ccATCGCCTGGCTGGTGCGCCCGGACCTGTTCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGt                                                                     <  2:431326/139‑1 (MQ=255)                                                        aTCGCCTGGCTGGTGCGCCCGGACCTGTTCACTCTCAAACCCTGTTTTGTGTCAGTGGAAACTCAGGGTGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAg                                                                       <  2:266203/135‑1 (MQ=255)                                                        aTCGCCTGGCTGGTGCGCCCGGACCTGTTCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAg                                                                       >  1:266203/1‑135 (MQ=255)                                                        aTCGCCTGGATGGTGCGCCCGGACCTGTTCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTgg                                                                   >  2:336486/1‑139 (MQ=255)                                                                    gTGCGCCCGGACCTGTTCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTgg                                                       >  1:229186/1‑139 (MQ=255)                                                                     tGCGCCCGGACCTGTTCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAagc                                                                                     >  2:114697/1‑108 (MQ=255)                                                                     tGCGCCCGGACCTGTTCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAagc                                                                                     <  1:114697/108‑1 (MQ=255)                                                                     tGCGCCCGGACCTGTTCACTCTCAAACCCTGTTTTGTGGCAGTAGAAACTCAGGGTGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAagcc                                                                                    <  1:208259/109‑1 (MQ=255)                                                                     tGCGCCCGGACCTGTTCACTCTCAAACCCTGTTTTGTGGCAGTAGAAACTCAGGGTGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAagcc                                                                                    >  2:208259/1‑109 (MQ=255)                                                                                   ttCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTcc                                        >  2:327259/1‑139 (MQ=255)                                                                                          tcgaaCCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAgtg                                 <  1:333542/136‑1 (MQ=255)                                                                                          tcAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGTGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAgtg                                 <  1:336486/139‑1 (MQ=255)                                                                                                                         gggTGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTaa  <  2:157108/139‑1 (MQ=255)                                                                                                                            |                                                                                                                                        CCACTACCGTAGCGGCAGTATGCAAAGCGGCTTGCGAATGCACGATCTCTGCGCCATCGCCTGGCTGGTGCGCCCGGACCTGTTCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGCGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGCCTGGGCAAGCCAGCCAATGTACAGGTGGCATTGGATCTGGATGTGAAAGGCTTCCAGCAGTGGGTGGCTGAGGTGCTGGCTCTGGCGTCGTAA  >  NC_000913/27949‑28207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑