Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,021,908 C→A R659L (CGC→CTC)  yccS ← putative transporter, FUSC superfamily inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,021,9080CA100.0% 48.4 / NA 15R659L (CGC→CTC) yccSputative transporter, FUSC superfamily inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (5/10);  total (5/10)

AATCTCAGGCAGTAATGCAATCAATAATCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCATCCGCTGTTTCAGGCTAGCTAATGCCTCATTGACGCGTTCTTCATCAGCAGGTTGATGATGTAACGCGTCATCAACATAGCAAACTGCGTCATCAAGAAACGCCAGAATTTCAGGATTAGTTAACTGCTCCCGGTGAGCACCGAGGGCT  >  NC_000913/1021787‑1022021
                                                                                                                         |                                                                                                                 
aaTCTCAGGCAGTAATGCAATCAATAATCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCATCCGCTGTTTCAGGCTAGCTAATGCCTCATTGACGAGTTCTTCATCAGCAGGt                                                                                                  <  1:191062/139‑1 (MQ=255)
aaTCTCAGGCAGTAATGCAATCAATAATCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCATCCGCTGTTTCAGGCTAGCTAATGCCTCATTGACGAGTTCTTCATCAGCAGGt                                                                                                  <  2:171726/139‑1 (MQ=255)
                   atcaatAATCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCATCCGCTGTTTCAGGCTAGCTAATGCCTCATTGACGAGTTCTTCATCAGCAGGTTTATGATGTAACGCGtcat                                                                               <  1:287035/139‑1 (MQ=255)
                                   gttgtACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCATCCGCTGTTTCAGGCTAGCTAATGCCTCATTGACGAGTTCTTCATCAGCAGGTTGATGATGTAACGCGTCATCAACATAGCAAACTGc                                                               >  1:67061/1‑139 (MQ=255)
                                                     cTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCATCCGCTGTTTCAGGCTAGCTAATGCCTCATTGACGAGTTCTTCATCAGCAGGTTGATGATGTAACGCGTCATCAACATAGCAAACTGCGTCATCAAGAAACGCCAg                                             >  1:41349/1‑139 (MQ=255)
                                                          cTGTCTGCCCGTGGTTCAAGTTGCTGCATCCGCTGTTTCAGGCTAGCTAATGCCTCATTGACGAGTTCTTCATCAGCAGGTTGATGATGTAACGCGTCATCAACATAGCAAACTGCGTCATCAAGAAACGCCAGAAttt                                        >  1:36366/1‑139 (MQ=255)
                                                             tCTGCCCGTGGTTCAAGTTGCTGCATCCGCTGTTTCAGGCTAGCTAATGCCTCATTGACGAGTTCTTCATCAGCAGGTTGATGATGTAACGCGTCATCAACATAGCAAACTGCGTCATCAAGAAACGCCAGAATTTCAg                                     <  2:36366/139‑1 (MQ=255)
                                                               tGCCCGTGGTTCAAGTTGCTGCATCCGCTGTTTCAGGCTAGCTAATGCCTCATTGACGAGTTCTTCATCAGCAGGTTGATGATGTAACGCGTCATCAACATAGCAAACTGCGtcatca                                                        >  1:407412/1‑118 (MQ=255)
                                                               tGCCCGTGGTTCAAGTTGCTGCATCCGCTGTTTCAGGCTAGCTAATGCCTCATTGACGAGTTCTTCATCAGCAGGTTGATGATGTAACGCGTCATCAACATAGCAAACTGCGtcatca                                                        <  2:407412/118‑1 (MQ=255)
                                                                    gTGGTTCAAGTTGCTGCATCCGCTGTTTCAGGCTAGCTAATGCCTCATTGACGAGttc                                                                                                               <  1:142430/58‑1 (MQ=255)
                                                                    gTGGTTCAAGTTGCTGCATCCGCTGTTTCAGGCTAGCTAATGCCTCATTGACGAGttc                                                                                                               >  2:142430/1‑58 (MQ=255)
                                                                       gTTCAAGTTGCTGCATCCGCTGTTTCAGGCTAGCTAATGCCTCATTGACGAGTTCTTCATCAGCAGGTTGATGATGTAACGCGTCATCAACATAGCAAACTGCGTCATCAAGAAACGCCAGAATTTCAGGATTAGTTaa                           <  1:205758/139‑1 (MQ=255)
                                                                                              ttCAGGCTAGCTAATGCCTCATTGACGAGTTCTTCATCAGCAGGTTGATGATGTAACGCGTCATCAACATAGCAAACTGCGTCATCAAGAAACGCCAGAATTTCAGGATTAGTTAACTGCTCCCGGTGAGCACCGAggg    <  2:41349/139‑1 (MQ=255)
                                                                                               tCAGGCTAGCTAATGCCTCATTGACGAGTTCTTCATCAGCAGGTTGATGATGTAACGCGTCATCAACATAGCAAACTGCGTCATCAAGAAACGCCAGAATTTCAGGATTAGTTAACTGCTCCCGGTGAGCACCGAGGGc   <  2:67061/139‑1 (MQ=255)
                                                                                                cAGGCTAGCTAATGCCTCATTGACGAGTTCTTCATCAGCAGGTTGATGATGTAACTCGTCATCAACATAGCAAACTGCGTCATCAAGAAACGCCAGAATTTCACGATTAGTTAACTGCTCCCGGTGAGCACCGAGGGCt  <  2:267131/139‑1 (MQ=255)
                                                                                                                         |                                                                                                                 
AATCTCAGGCAGTAATGCAATCAATAATCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCATCCGCTGTTTCAGGCTAGCTAATGCCTCATTGACGCGTTCTTCATCAGCAGGTTGATGATGTAACGCGTCATCAACATAGCAAACTGCGTCATCAAGAAACGCCAGAATTTCAGGATTAGTTAACTGCTCCCGGTGAGCACCGAGGGCT  >  NC_000913/1021787‑1022021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: