Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,398,875	A→G	F218L (TTC→CTC)	fnr ←	oxygen‑sensing anaerobic growth regulon transcriptional regulator FNR, autorepressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,398,875	0	A	G	100.0%	53.6 / NA	15	F218L (TTC→CTC)	fnr	oxygen‑sensing anaerobic growth regulon transcriptional regulator FNR, autorepressor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/10);  total (5/10)

AGGATAGTGAGTTATGCGGAAAAATCAGGCAACGTTACGCGTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGG  >  NC_000913/1398750‑1398951                                                                                                                              |                                                                             aGGATAGTGAGTTATGCGGAAAAATCAGGCAACGTTACGCGTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAGGCGACCCAGCAGa                                                                 <  1:153876/139‑1 (MQ=255)   gATAGTGAGTTATGCGGAAAAATCAGGCAACGTTACGCGTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAGGCGACCCAGCAGACg                                                               <  1:306553/139‑1 (MQ=255)       gTGAGTTATGCGGAAAAATCAGGCAACGTTACGCGTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAGGCGACCCAGCAGACGGCTg                                                           <  2:482089/139‑1 (MQ=255)              aTGCGGAAAAATCAGGCAACGTTACGCGTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAGGc                                                                            <  1:163823/115‑1 (MQ=255)              aTGCGGAAAAATCAGGCAACGTTACGCGTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAGGc                                                                            >  2:163823/1‑115 (MQ=255)               tGCGGAAAAATCAGGCAACGTTACGCGTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAGGCGACCcagcag                                                                  <  1:470204/124‑1 (MQ=255)               tGCGGAAAAATCAGGCAACGTTACGCGTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAGGCGACCcagcag                                                                  >  2:470204/1‑124 (MQ=255)               tGCGGAAAAATCAGGCAACGTTACGCGTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAGGCGACCCAGCAGACGGCTGATGGTTTc                                                   >  2:155019/1‑139 (MQ=255)               tGCGGAAAAATCAGGCAACGTTACGCGTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAGGCGACCCAGCAGACGGCTGATGGTTTc                                                   >  2:207607/1‑139 (MQ=255)                    aaaaaTCAGGCAACGTTACGCGTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAGGCGACCCAGCAGACGGCTGATGGTTTCTACCg                                              >  1:117131/1‑139 (MQ=255)                     aaaaTCAGGCAACGTTACGCGTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAGGCGACCCAGCAGACGGCTGATGGTTTCTACCGt                                             <  1:207607/139‑1 (MQ=255)                     aaaaTCAGGCAACGTTACGCGTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAGGCGACCCAGCAGACGGCTGATGGTTTCTACCGt                                             <  2:126343/139‑1 (MQ=255)                           aGGCAACGTTACGCGTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAGGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGcc                                       <  2:117131/139‑1 (MQ=255)                                      cgcgTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAGGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTAc                            <  1:155019/139‑1 (MQ=255)                                                                cgcATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAGGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATTGTCAgg  <  1:95696/139‑1 (MQ=255)                                                                                                                              |                                                                             AGGATAGTGAGTTATGCGGAAAAATCAGGCAACGTTACGCGTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGG  >  NC_000913/1398750‑1398951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑