Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,613,082	T→C	D57G (GAC→GGC)	glsB ←	glutaminase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,613,082	0	T	C	100.0%	46.1 / NA	13	D57G (GAC→GGC)	glsB	glutaminase 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/8);  total (5/8)

ACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGTAATGACGCATGGCGACAACGAGACTCAGCACTTTGGAAATAGACTGAATGGAAAAACGTTCTTGCGCGTCTCCGGCCTGAAAAAGCTGTCCGTCAACGGTACAGATAGCAATCCCCAATCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAG  >  NC_000913/1612960‑1613213                                                                                                                           |                                                                                                                                    aCGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGTAATGACGCATGGCGACAACGAGACTCAGCACTTTGGAAATAGACTGAATGGAAAAACGTTCTTGCGCGCCTCCGGCCTGAAAAAg                                                                                                                     <  2:416426/139‑1 (MQ=255)                                      gATTTCCTCTTCGGAGTAATGACGCATGGCGACAACGAGACTCAGCACTTTGGAAATAGACTGAATGGAAAAACGTTCTTGCGCGCCTCCGGCCTGAAAAAGCTGTCCGTCAACGGTACAGATAGCAATCCCCAATCgg                                                                                <  2:171353/139‑1 (MQ=255)                                             tctTCGGAGTAATGACGCATGGCGACAACGAGACTCAGCACTTTGGAAATAGACTGAATGGAAAAACGTTCTTGCGCGCCTCCGGCCTGAAAAAGCTGTCCGTCAACGGTACAGATAGCAATCCCCAATCGGGAACCGt                                                                         <  1:190139/139‑1 (MQ=255)                                             tctTCGGAGTAATGACGCATGGCGACAACGAGACTCAGCACTTTGGAAATAGACTGAATGGAAAAACGTTCTTGCGCGCCTCCGGCCTGAAAAAGCTGTCCGTCAACGGTACAGATAGCAATCCCCAATCGGGAACCGt                                                                         <  1:83951/139‑1 (MQ=255)                                                              cATGGCGACAACGAGACTCAGCACTTTGGAAATAGACTGAATGGAAAAACGTTCTTGCGCGCCTCCGGCCTGAAAAAGCTGTCCGTCAACGGTACAGATAGCAATCCCCAATCGGGAACCGTCTACTGTAGCCAGCGcc                                                        >  1:145987/1‑139 (MQ=255)                                                                tGGCGACAACGAGACTCAGCACTTTGGAAATAGACTGAATGGAAAAACGTTCTTGCGCGCCTCCGGCCTGAAAAAGCTGTCCGTCAACGGTACAGATAGCAATCCCCAATCGGGAACCGTCTACTGTAGCCAGCGCCgg                                                      >  1:157752/1‑139 (MQ=255)                                                                tGGCGACAACGAGACTCAGCACTTTGGAAATAGACTGAATGGAAAAACGTTCTTGCGCGCCTCCGGCCTGAAAAAGCTGTCCGTCAACGGTACAGATAGCAATCCCCAATCGGGAACCGTCTACTGTAGCCAGCGCCgg                                                      >  2:59396/1‑139 (MQ=255)                                                                                cAGCACTTTGGAAATAGACTGAATGGAAAAACGTTCTTGCGCGCCTCCGGCCTGAAAAAGCTGTCCGTCAACGGTACAGATAGCAATCCCCAATCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACt                                      <  1:186186/139‑1 (MQ=255)                                                                                        tGGAAATAGACTGAATGGAAAAACGTTCTTGCGCGCCTCCGGCCTGAAAAAGCTGTCCGTCAACGGTACAGATAGCAATCCCCAATCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTg                              >  2:241909/1‑139 (MQ=255)                                                                                                gACTGAATGGAAAAACGTTCTTGCGCGCCTCCGGCCTGAAAAAGCTGTCCGTCAACGGTACAGATAGCAATCCCCAATCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGa                      <  1:59396/139‑1 (MQ=255)                                                                                                gACTGAATGGAAAAACGTTCTTGCGCGCCTCCGGCCTGAAAAAGCTGTCCGTCAACGGTACAGATAGCAATCCCCAATCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGa                      <  2:145987/139‑1 (MQ=255)                                                                                                               cGTTCTTGCGCGCCTCCGGCCTGAAAAAGCTGTCCGTCAACGGTACAGATAGCAATCCCCAATCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGcc       >  2:143497/1‑139 (MQ=255)                                                                                                                    ttGCGCGCCTCCGGCCTGAAAAAGCTGTCCGTCAACGGTACAGATAGCAATCCCCAATCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAg  <  1:143497/139‑1 (MQ=255)                                                                                                                           |                                                                                                                                    ACGGTGATCCAGACGGATCTTTGCCGACGCGTTGCCAGATTTCCTCTTCGGAGTAATGACGCATGGCGACAACGAGACTCAGCACTTTGGAAATAGACTGAATGGAAAAACGTTCTTGCGCGTCTCCGGCCTGAAAAAGCTGTCCGTCAACGGTACAGATAGCAATCCCCAATCGGGAACCGTCTACTGTAGCCAGCGCCGGAATATAATCCGCGACTTTACCCTGACCAATGAGCGGCCGCACTTGCCGCAAG  >  NC_000913/1612960‑1613213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑