Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,907,616	C→T	intergenic (‑25/+645)	yobF ← / ← yebO	DUF2527 family heat‑induced protein/putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,907,616	0	C	T	100.0%	121.3 / NA	36	intergenic (‑25/+645)	yobF/yebO	DUF2527 family heat‑induced protein/putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/16);  total (20/16)

CATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTCTTCCACTTTTTTTGCACACTTATGCAACGGAATACGCGCCGATATA  >  NC_000913/1907503‑1907746                                                                                                                  |                                                                                                                                   cATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGt                                                                                                           >  1:66774/1‑139 (MQ=255)      ggCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAAtt                                                                                                      >  2:446128/1‑139 (MQ=255)      ggCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAAtt                                                                                                      >  1:41620/1‑139 (MQ=255)               ataAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGc                                                                                             <  2:41620/139‑1 (MQ=255)               ataAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGc                                                                                             <  1:446128/139‑1 (MQ=255)                     cTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAAt                                                                                                       >  1:46071/1‑123 (MQ=255)                     cTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAAt                                                                                                       <  2:46071/123‑1 (MQ=255)                        ggCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTg                                                                                                                           >  2:392396/1‑100 (MQ=255)                        ggCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTg                                                                                                                           <  1:392396/100‑1 (MQ=255)                        ggCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTaa                                                                                    >  2:40712/1‑139 (MQ=255)                        ggCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTaa                                                                                    >  2:21647/1‑139 (MQ=255)                        ggCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTaa                                                                                    >  2:79892/1‑139 (MQ=255)                                    gTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGACCTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGAt                                                                        <  2:388724/139‑1 (MQ=255)                                    gTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATa                                                                                                                 >  1:403437/1‑98 (MQ=255)                                    gTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATa                                                                                                                 <  2:403437/98‑1 (MQ=255)                                       ttCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAg                                                                     >  1:33087/1‑139 (MQ=255)                                               caacgtGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACaa                                                             >  2:415777/1‑139 (MQ=255)                                                 acgtGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATc                                                                                                                      <  1:224145/80‑1 (MQ=255)                                                 acgtGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATc                                                                                                                      >  2:224145/1‑80 (MQ=255)                                                              ggACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAGCATGTCTCGAGCAGACGCACAAATCTCGATACGTCtt                                              >  1:320027/1‑139 (MQ=255)                                                               gACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGc                                                                                             >  1:43910/1‑91 (MQ=255)                                                               gACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGc                                                                                             <  2:43910/91‑1 (MQ=255)                                                                          tGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTCTTCCACTTTTTTTg                                  <  1:79892/139‑1 (MQ=255)                                                                          tGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTCTTCCACTTTTTTTg                                  <  2:193519/139‑1 (MQ=255)                                                                            aaaaTGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCTGACGCACAAATCTCGATACGTCTTCCACTTTTTTTGca                                <  2:66774/139‑1 (MQ=255)                                                                            aaaaTGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTCTTCCACTTTTTTTGca                                >  2:408199/1‑139 (MQ=255)                                                                            aaaaTGCCACACATAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGACTTCCACTTTTTTTGca                                >  2:171483/1‑139 (MQ=255)                                                                                     cacaTAAACAGAACTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTCTTCCACTTTTTTTGCACACTTATGc                       <  1:40712/139‑1 (MQ=255)                                                                                                aaCTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTCTTCCACTTTTTTTGCACACTTATGCAACGGAATAcg            >  1:281897/1‑139 (MQ=255)                                                                                                aaCTGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGCCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCAATACGTCTACCACTTTTTTTGCACACTTATGCAACGGAATAcg            >  1:44374/1‑139 (MQ=255)                                                                                                   tGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTCTTCCACTTTTTTTGCACACTTATGCAACGGAATACgcgc         <  2:33087/139‑1 (MQ=255)                                                                                                   tGTACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTCTTCCACTTTTTTTGCACACTTATGCAACGGAATACgcgc         <  1:171483/139‑1 (MQ=255)                                                                                                     tACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTCTTCCACTTTTTTTGcac                               <  1:49461/115‑1 (MQ=255)                                                                                                     tACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTCTTCCACTTTTTTTGcac                               >  2:49461/1‑115 (MQ=255)                                                                                                     tACCTCGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTCTTCCACTTTTTTTGCACACTTATGCAACGGAATACGCGCCg       <  1:408199/139‑1 (MQ=255)                                                                                                          cGTTTAACTCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTCTTCCACTTTTTTTGCACACTTATGCAACGGAATACGCGCCGatata  >  1:12855/1‑139 (MQ=255)                                                                                                                  |                                                                                                                                   CATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACCCGAAATCTGTTATCACATACAACGTTAATTATGGCAAGCCATTTTTAAACATGTCTCGATCAGACGCACAAATCTCGATACGTCTTCCACTTTTTTTGCACACTTATGCAACGGAATACGCGCCGATATA  >  NC_000913/1907503‑1907746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑