Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,024,017	C→T	T17I (ACC→ATC)	rcsA →	transcriptional regulator of colanic acid capsular biosynthesis

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,024,017	0	C	T	100.0%	195.9 / NA	57	T17I (ACC→ATC)	rcsA	transcriptional regulator of colanic acid capsular biosynthesis
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/28);  total (29/28)

TGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGTAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAACCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCAACGACATTGAAACCGTTGATGACCTTGCCATAGCTTGTGATTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTTTCATCCACGAT  >  NC_000913/2023888‑2024150                                                                                                                                  |                                                                                                                                      tGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGTAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAATCGGGTATCt                                                                                                                              <  2:147755/139‑1 (MQ=255)  gACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGTAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAATCGGGTATCTg                                                                                                                             <  2:258889/139‑1 (MQ=255)  gACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGTAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAATCGGGTATCTg                                                                                                                             <  1:32178/139‑1 (MQ=255)    cccATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGTAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAATCGGGTATCTGtt                                                                                                                           >  2:494115/1‑139 (MQ=255)       aTGTTGTTCCGTTTAGTCATGATGAAATATTCAGGTAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAATCGGGTATCTGTTg                                                                                                                          >  2:80745/1‑137 (MQ=255)       aTGTTGTTCCGTTTAGTCATGATGAAATATTCAGGTAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAATCGGGTATCTGTTg                                                                                                                          <  1:80745/137‑1 (MQ=255)             ttCCGTTTAGTCATGATGAAATATTCAGGTAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAATCGGGTATCTGTTGAGTAGAgg                                                                                                                  >  2:329786/1‑139 (MQ=255)                  tttAGTCATGATGAAATATTCAGGTAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAATCggg                                                                                                                                   <  2:351065/117‑1 (MQ=255)                  tttAGTCATGATGAAATATTCAGGTAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAATCggg                                                                                                                                   >  1:351065/1‑117 (MQ=255)                    tAGTCATGATGAAATATTCAGGTAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAATCGGGTATCTGTTGAGTAGAGGGGTTaaa                                                                                                           >  2:4230/1‑139 (MQ=255)                                  tatTCAGGTAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAATCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCaa                                                                                             <  1:494115/139‑1 (MQ=255)                                                                 tgagtgagGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAATCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCAACGACATTGAAACCGTTGATGACCTTGCCATa                                                              >  1:303569/1‑139 (MQ=255)                                                                   agtgagGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAATCGGGTATCTGTTGAGTa                                                                                                                      >  2:16355/1‑81 (MQ=255)                                                                   agtgagGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAATCGGGTATCTGTTGAGTa                                                                                                                      <  1:16355/81‑1 (MQ=255)                                                                   agtgagGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAATCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCAACGACATTGAAACCGTTGATGACCTTGCCATAGc                                                            >  1:412146/1‑139 (MQ=255)                                                                   agtgagGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAATCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCAACGACATTGAAACCGTTGATGACCTTGCCATAGc                                                            >  2:277546/1‑139 (MQ=255)                                                                   agtgagGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAATCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCAACGACATTGAAACCGTTGATGACCTTGCCATAGc                                                            <  1:277546/139‑1 (MQ=255)                                                                      gagGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAATCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCAACGACATTGAAACCGTTGATGACCTTGCCATAGCTtg                                                         >  2:287309/1‑139 (MQ=255)                                                                             tGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAATCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCAACGACATTGAAACCGTTGATGACCTTGCCATAGCTTGTGATTca                                                  <  1:384487/139‑1 (MQ=255)                                                                                  tGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAATCGGGt                                                                                                                                  >  1:145375/1‑54 (MQ=255)                                                                                  tGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAATCGGGt                                                                                                                                  <  2:145375/54‑1 (MQ=255)                                                                                   gTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAATCGGGTATCTg                                                                                                                             <  2:444412/58‑1 (MQ=255)                                                                                   gTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAATCGGGTATCTg                                                                                                                             >  1:444412/1‑58 (MQ=255)                                                                                   gTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAATCGGGTATCTGTTGAGTag                                                                                                                     >  1:351854/1‑66 (MQ=255)                                                                                   gTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAATCGGGTATCTGTTGAGTag                                                                                                                     <  2:351854/66‑1 (MQ=255)                                                                                   gTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAATCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCAACGACATTGAAACCGTtga                                                                           <  1:367341/108‑1 (MQ=255)                                                                                   gTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAATCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCAACGACATTGAAACCGTtga                                                                           >  2:367341/1‑108 (MQ=255)                                                                                   gTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAATCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCAACGACATTGAAACCGTTGATGACCTTGCCATAGCTTGTGATTcaca                                                >  2:371990/1‑135 (MQ=255)                                                                                   gTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAATCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCAACGACATTGAAACCGTTGATGACCTTGCCATAGCTTGTGATTcaca                                                <  1:371990/135‑1 (MQ=255)                                                                                   gTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAATCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCAACGACATTGAAACCGTTGATGACCTTGCCATAGCTTGTGATTCACAgcgc                                            >  1:424000/1‑139 (MQ=255)                                                                                   gTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAATCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCAACGACATTGAAACCGTTGATGACCTTGCCATAGCTTGTGATTCACAgcgc                                            >  2:257313/1‑139 (MQ=255)                                                                                    tCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAATCGGGt                                                                                                                                  >  1:230039/1‑52 (MQ=255)                                                                                    tCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAATCGGGt                                                                                                                                  <  2:230039/52‑1 (MQ=255)                                                                                            tattatGGATTTATGTAGTTACACCCGACTAGGTTTAATCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCAACGACATTGAAACCGTTGATGACCTTGCCATAGCTTGTGATTCACAGCGCCCTTCAgtg                                   <  2:303569/139‑1 (MQ=255)                                                                                                     tttATGTAGTTACACCCGACTAGGTTTAATCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCAACGACATTGAAACCGTTGATGACCTTGCCATAGCTTGTGATTCACAg                                               <  1:333126/118‑1 (MQ=255)                                                                                                     tttATGTAGTTACACCCGACTAGGTTTAATCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCAACGACATTGAAACCGTTGATGACCTTGCCATAGCTTGTGATTCACAg                                               >  2:333126/1‑118 (MQ=255)                                                                                                        aTGTAGTTACACCCGACTAGGTTTAATCGGGTATCTGtt                                                                                                                           <  2:212166/39‑1 (MQ=255)                                                                                                        aTGTAGTTACACCCGACTAGGTTTAATCGGGTATCTGtt                                                                                                                           >  1:212166/1‑39 (MQ=255)                                                                                                         tGTAGTTACACCCGACTAGGTTTAATCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCAACGACATTGAAACCGTTGATGACCTTGCCATAGCTTGTGATTCACAgcg                                             >  2:213906/1‑116 (MQ=255)                                                                                                         tGTAGTTACACCCGACTAGGTTTAATCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCAACGACATTGAAACCGTTGATGACCTTGCCATAGCTTGTGATTCACAgcg                                             <  1:213906/116‑1 (MQ=255)                                                                                                             gTTACACCCGACTAGGTTTAATCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAag                                                                                                      >  1:203047/1‑55 (MQ=255)                                                                                                             gTTACACCCGACTAGGTTTAATCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAag                                                                                                      <  2:203047/55‑1 (MQ=255)                                                                                                              ttACACCCGACTAGGTTTAATCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCAACGACATTGaaa                                                                                  >  1:387091/1‑74 (MQ=255)                                                                                                              ttACACCCGACTAGGTTTAATCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCAACGACATTGaaa                                                                                  <  2:387091/74‑1 (MQ=255)                                                                                                                   cccGACTAGGTTTAATCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCAACGACATTGAAACCGTTGATGACCTTGCCATAGCTTGTGATTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGttt            <  2:497328/139‑1 (MQ=255)                                                                                                                   cccGACTAGGTTTAATCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCAACGACATTGAAACCGTTGATGACCTTGCCATAGCTTGTGATTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGttt            <  2:412146/139‑1 (MQ=255)                                                                                                                      gACTAGGTTTAATCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCAACGACATTGAAACCGTTGATGACCTTGCCATAGCTTGTGATTCACAGCGCCCTTCAGTGGTGTtta                            >  1:428919/1‑120 (MQ=255)                                                                                                                      gACTAGGTTTAATCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCAACGACATTGAAACCGTTGATGACCTTGCCATAGCTTGTGATTCACAGCGCCCTTCAGTGGTGTtta                            <  2:428919/120‑1 (MQ=255)                                                                                                                       aCTAGGTTTAATCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCAACGACATTGaaa                                                                                  <  1:427401/65‑1 (MQ=255)                                                                                                                       aCTAGGTTTAATCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCAACGACATTGaaa                                                                                  >  2:427401/1‑65 (MQ=255)                                                                                                                       aCTAGGTTTAATCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCAACGACATTGAAACCGTTGATGACCTTGCCATAGCTTGTGATTCACAGCGCCCTTCAGTGGTGTtta                            <  2:247601/119‑1 (MQ=255)                                                                                                                       aCTAGGTTTAATCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCAACGACATTGAAACCGTTGATGACCTTGCCATAGCTTGTGATTCACAGCGCCCTTCAGTGGTGTtta                            >  1:247601/1‑119 (MQ=255)                                                                                                                       aCTAGGTTTAATCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCAACGACATTGAAACCGTTGATGACCTTGCCATAGCTTGTGATTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTTTCATc        >  2:171198/1‑139 (MQ=255)                                                                                                                       aCTAGGTTTAATCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCAACGACATTGAAACCGTTGATGACCTTGCCATAGCTTGTGATTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTTTCATc        >  2:286219/1‑139 (MQ=255)                                                                                                                        cTAGGTTTAATCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCAACGACATTGAAACCGTTGATGACCTTGCCATAGCTTGTGATTCACAGCGCCCTTCAgtg                                   >  2:22641/1‑111 (MQ=255)                                                                                                                        cTAGGTTTAATCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCAACGACATTGAAACCGTTGATGACCTTGCCATAGCTTGTGATTCACAGCGCCCTTCAgtg                                   <  1:22641/111‑1 (MQ=255)                                                                                                                             tttAATCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCAACGACATTGAAACCGTTGATGACCTTGCCATAGCTTGTGATTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTTTCATCCACGAt  <  1:257382/139‑1 (MQ=255)                                                                                                                                  |                                                                                                                                      TGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGTAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAACCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCAACGACATTGAAACCGTTGATGACCTTGCCATAGCTTGTGATTCACAGCGCCCTTCAGTGGTGTTTATTAATGAGGACTGTTTCATCCACGAT  >  NC_000913/2023888‑2024150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑