Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,457,127 A→T M678K (ATG→AAG)  fadJ ← enoyl‑CoA hydratase/epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,457,1270AT100.0% 77.4 / NA 23M678K (ATG→AAG) fadJenoyl‑CoA hydratase/epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (10/13);  total (10/13)

GATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCA  >  NC_000913/2456993‑2457258
                                                                                                                                      |                                                                                                                                   
gATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCtttat                                                                                                                                 <  2:430262/139‑1 (MQ=255)
         aGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCTTTATTGCAACCAc                                                                                                                        <  1:185617/139‑1 (MQ=255)
           cTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCTTTATTGCAACCACtt                                                                                                                      >  1:458349/1‑139 (MQ=255)
             ttGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCTTTATTGCAACCACTTCg                                                                                                                    >  2:318362/1‑139 (MQ=255)
              tGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCTTTATTGCAACCACTTCGc                                                                                                                   <  2:5568/139‑1 (MQ=255)
               gACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCTTTATTGCAACCACTTCGcc                                                                                                                  >  1:494730/1‑139 (MQ=255)
                     cttATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCTTTATTGCAACCACTTCGCCCGCGcc                                                                                                            >  1:372077/1‑139 (MQ=255)
                        aTTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCTTTATTGCAACCACTTCGCCCGCGCCgag                                                                                                         <  2:486732/139‑1 (MQ=255)
                             aGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCTTTATTGCAACCACTTCGCCCGCGCCGAGAGAAt                                                                                                    <  2:458349/139‑1 (MQ=255)
                                    ttgcagttgTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCTTACTGCGTGGCAAGTCGTTGCTTTATTGCAACCACTTCGCCCGCGCCGAGAGAAt                                                                                                    >  1:358531/1‑132 (MQ=255)
                                    ttgcagttgTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCTTTATTGCAACCACTTCGCCCGCGCCGAGAGAAt                                                                                                    <  2:358531/132‑1 (MQ=255)
                                         gttgttTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCTTTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGa                                                                                        <  2:432690/139‑1 (MQ=255)
                                                           tCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCTTTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATg                                                                      >  2:469040/1‑139 (MQ=255)
                                                                      gcCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCTTTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCa                                                           <  1:318362/139‑1 (MQ=255)
                                                                                aCCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCTTTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATa                                                 <  2:372077/139‑1 (MQ=255)
                                                                                aCCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCTTTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATa                                                 >  1:237473/1‑139 (MQ=255)
                                                                                   aaaCGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCTTTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACcg                                              <  1:469040/139‑1 (MQ=255)
                                                                                                                        tGGCAAGTCGTTGCTTTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAatat                                     <  1:387965/111‑1 (MQ=255)
                                                                                                                        tGGCAAGTCGTTGCTTTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAatat                                     >  2:387965/1‑111 (MQ=255)
                                                                                                                        tGGCAAGTCGTTGCTTTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTg         >  1:379863/1‑139 (MQ=255)
                                                                                                                        tGGCAAGTCGTTGCTTTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTg         <  2:494730/139‑1 (MQ=255)
                                                                                                                         ggCAAGTCGTTGCTTTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGc        >  1:228933/1‑139 (MQ=255)
                                                                                                                             aGTCGTTGCTTTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCtcat    <  2:379863/139‑1 (MQ=255)
                                                                                                                               tCGTTGCTTTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCtcatca  <  2:237473/139‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                   
GATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCA  >  NC_000913/2456993‑2457258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: