Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,624,129	C→T	F362F (TTC→TTT)	ppk →	polyphosphate kinase, component of RNA degradosome

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,624,129	0	C	T	100.0%	90.4 / NA	27	F362F (TTC→TTT)	ppk	polyphosphate kinase, component of RNA degradosome
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/11);  total (16/11)

TTCCGCAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCG  >  NC_000913/2624028‑2624262                                                                                                      |                                                                                                                                      ttCCGCAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGAc                                                                                                                                    <  1:387773/105‑1 (MQ=255) ttCCGCAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGAc                                                                                                                                    >  2:387773/1‑105 (MQ=255)      cAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTg                                                                                             >  1:105090/1‑139 (MQ=255)                     cATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGc                                                                              >  2:24093/1‑139 (MQ=255)                      aTTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGca                                                                             >  1:226087/1‑139 (MQ=255)                       ttCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGcat                                                                            >  2:129735/1‑139 (MQ=255)                                                ttatCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGAtt                                                                                                                  <  1:21491/76‑1 (MQ=255)                                                ttatCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGAtt                                                                                                                  >  2:21491/1‑76 (MQ=255)                                                ttatCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACt                                                                    >  1:151780/1‑122 (MQ=255)                                                ttatCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACt                                                                    <  2:151780/122‑1 (MQ=255)                                                   tCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACAt                                                <  1:24093/139‑1 (MQ=255)                                                   tCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACAt                                                >  1:50086/1‑139 (MQ=255)                                                        aTCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACgg                                           >  2:267737/1‑139 (MQ=255)                                                             acCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGa                                      >  2:367313/1‑139 (MQ=255)                                                              ccTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGaa                                     <  2:226087/139‑1 (MQ=255)                                                               cTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGaaa                                    >  1:286495/1‑139 (MQ=255)                                                                 ttGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCAc                                                                                >  1:42023/1‑93 (MQ=255)                                                                 ttGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCAc                                                                                <  2:42023/93‑1 (MQ=255)                                                                    aGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCAc                               <  2:286495/139‑1 (MQ=255)                                                                    aGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCAc                               <  1:129735/139‑1 (MQ=255)                                                                         gtgCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACAt                                                <  1:496816/117‑1 (MQ=255)                                                                         gtgCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACAt                                                >  2:496816/1‑117 (MQ=255)                                                                         gtgCTGGAACTGCTGCGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCgtgg                          >  1:264559/1‑139 (MQ=255)                                                                                    gctgcGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTa               >  2:386400/1‑139 (MQ=255)                                                                                    gctgcGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTa               <  2:50086/139‑1 (MQ=255)                                                                                     ctgcGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAAtt                                                                                                            >  2:278064/1‑45 (MQ=255)                                                                                     ctgcGTCAGGCTTCGTTTGACCCGAGCGTACTGGCGATTAAAAtt                                                                                                            <  1:278064/45‑1 (MQ=255)                                                                                                 tcgtttgACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTcg  <  1:386400/139‑1 (MQ=255)                                                                                                      |                                                                                                                                      TTCCGCAATGGTTTTGATGCCATTCGCGAACGCGATGTGTTGCTCTATTATCCTTATCACACCTTTGAGCATGTGCTGGAACTGCTGCGTCAGGCTTCGTTCGACCCGAGCGTACTGGCGATTAAAATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCG  >  NC_000913/2624028‑2624262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑