Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,689,476 G→A R11* (CGA→TGA)  yfhG ← putative outer membrane protein modulating the QseEF response

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,689,4760GA100.0% 74.9 / NA 23R11* (CGA→TGA) yfhGputative outer membrane protein modulating the QseEF response
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/12);  total (11/12)

TAGTCAGCCAGTTGATAAACCGGAATTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAGCAATGCCAGACATGGCAGCCCTGCCAGCCACAGCCGTCGGGGCAATAATCGTTGAAAAATGTGTCGCATATTCACCAGACTTAAAGCCTATCCCAGTGGGCGTAATTGTTGCAGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCG  >  NC_000913/2689355‑2689597
                                                                                                                         |                                                                                                                         
tAGTCAGCCAGTTGATAAACCGGAATTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAGCAATGCCAGACATGGCAGCCCTGCCAGCCACAGCCGTCAGGGCAATAATCGTTGaa                                                                                                          >  1:370010/1‑139 (MQ=255)
  gTCAGCCAGTTGATAAACCGGAATTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAGCAATGCCAGACATGGCAGCCCTGCCAGCCACAGCCGTCAGGGCAATAATCGTTGaaaa                                                                                                        >  1:412836/1‑139 (MQ=255)
  gTCAGCCAGTTGATAAACCGGAATTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAGCAATGCCAGACATGGCAGCCCTGCCAGCCACAGCCGTCAGGGCAATAATCGTTGaaaa                                                                                                        >  2:306671/1‑139 (MQ=255)
    cAGCCAGTTGATAAACCGGAATTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAGCAATGCCAGACATGGCAGCCCTGCCAGCCACAGCCGTCAGGGCAATAATCGTTGAAAAAt                                                                                                      <  2:370010/139‑1 (MQ=255)
                    cGGAATTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAGCAATGCCAGACATGGCAGCCCTGCCAGCCACAGCCGTCAGGGCAATAATCGTTGAAAAATGTGTCGCATATTCAcc                                                                                      <  2:412836/139‑1 (MQ=255)
                          tttttCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAGCAATGCCAGACATGGCAGCCCTGCCAGCCACAGCCGTCAGGGCAATAATCGTTGAAAAATGTGTCGCATATTCACCAGACtt                                                                                <  1:306671/139‑1 (MQ=255)
                                                     tggctTATTGTGATTCTGCACGCAACCCAGCAATGCCAGACATGGCAGCCCTGCCAGCCACAGCCGTCAGGGCAATAATCGTTGAAAAATGTGTCGCATATTCACCa                                                                                     >  2:343234/1‑107 (MQ=255)
                                                     tggctTATTGTGATTCTGCACGCAACCCAGCAATGCCAGACATGGCAGCCCTGCCAGCCACAGCCGTCAGGGCAATAATCGTTGAAAAATGTGTCGCATATTCACCa                                                                                     <  1:343234/107‑1 (MQ=255)
                                                       gctTATTGTGATTCTGCACGCAACCCAGCAATGCCAGACATGGCAGCCCTGCCAGCCACAGCCGTCAGGGCAATAATCGTTGAAAAATGTGTCGCATATTCACCAGACTTAAAGCCTATCCCAGTGGGCGTAAttgttg                                                   >  1:209031/1‑139 (MQ=255)
                                                            ttGTGATTCTGCACGCAACCCAGCAATGCCAGACATGGCAGCCCTGCCAGCCACAGCCGTCAGGGCAATAATCGTTGAAAAATGTGTCGCATATTCACCAGACTTAAAGCCTATCCCAGTGGGCGTAATTGTTGCAGAc                                              <  1:156091/139‑1 (MQ=255)
                                                                    cTGCACGCAACCCAGCAATGCCAGACATGGCAGCCCTGCCAGCCACAGCCGTCAGGGCAATAATCGTTGAAAAATGTGTCGCATATTCACCAGACTTAAAGCCTATCCCAGTGGGCGTAATTGTTGCAGACAGTCtgga                                      >  1:2236/1‑139 (MQ=255)
                                                                                      tGCCAGACATGGCAGCCCTGCCAGCCACAGCCGTCAGGGCAATAATCGTTGAAAAATGTGTCGCATATTCACCAGACTTAAAGCCTATCCCAGTGGGCGTAATTGTTGCAGACAGTCTGGACATGGACAGCGCGGAGaa                    >  2:451046/1‑139 (MQ=255)
                                                                                        ccAGACATGGCAGCCCTGCCAGCCACAGCCGTCAGGGCAATAATCGTTGAAAAATGTGTCGCATATTCACCAGACTTAAAGCCTATCCCAGTGGGCGTAATTGTTGCAg                                                >  2:30042/1‑109 (MQ=255)
                                                                                        ccAGACATGGCAGCCCTGCCAGCCACAGCCGTCAGGGCAATAATCGTTGAAAAATGTGTCGCATATTCACCAGACTTAAAGCCTATCCCAGTGGGCGTAATTGTTGCAg                                                <  1:30042/109‑1 (MQ=255)
                                                                                             cATGGCAGCCCTGCCAGCCACAGCCGTCAGGGCAATAATCGTTGAAAAATGTGTCGCATATTCACCAGACTTAAAGCCTATCCCAGTGGGCGTAATTGTTGCAGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAg             <  2:2236/139‑1 (MQ=255)
                                                                                             cATGGCAGCCCTGCAAGCCACAGCCGTCAGGGCAATAATCGTTGAAAAATGTGTCGCATATTCACCAGACTTAAAGCCTATCCCAGTGGGCGTAATTGTTGCAGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAg             <  1:29140/139‑1 (MQ=255)
                                                                                               tGGCAGCCCTGCCAGCCACAGCCGTCAGGGCAATAATCGTTGAAAAATGTGTCGCATATTCACCAGACTTAAAGCCTATCCCAGTGGGCGTAATTGTTGCAGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCg           <  1:451046/139‑1 (MQ=255)
                                                                                                ggCAGCCCTGCCAGCCACAGCCGTCAGGGCAATAATCGTTGAAAAATGTGTCGCATATTCACCAGACTTa                                                                               >  2:247645/1‑70 (MQ=255)
                                                                                                ggCAGCCCTGCCAGCCACAGCCGTCAGGGCAATAATCGTTGAAAAATGTGTCGCATATTCACCAGACTTa                                                                               <  1:247645/70‑1 (MQ=255)
                                                                                                  cAGCCCTGCCAGCCACAGCCGTCAGGGCAATAATCGTTGAAAAATGTGTCGCATATTCACCAGACTTAAAGCCTATCCCAGTGGGCGTAATTGTTGCAGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTAc        >  2:356569/1‑139 (MQ=255)
                                                                                                        tGCCAGCCACAGCCGTCAGGGCAATAATCGTTGAAAAATGTGTc                                                                                                 <  2:263084/44‑1 (MQ=255)
                                                                                                        tGCCAGCCACAGCCGTCAGGGCAATAATCGTTGAAAAATGTGTc                                                                                                 >  1:263084/1‑44 (MQ=255)
                                                                                                        tGCCAGCCACAGCCGTCAGGGCAATAATCGTTGAAAAATGTGTCGCATATTCACCAGACTTAAAGCCTATCCCAGTGGGCGTAATTGTTGCAGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCg  <  2:209031/139‑1 (MQ=255)
                                                                                                                         |                                                                                                                         
TAGTCAGCCAGTTGATAAACCGGAATTTTTTCTTCTGCTGGCGTATCAATGGCTGGCTTATTGTGATTCTGCACGCAACCCAGCAATGCCAGACATGGCAGCCCTGCCAGCCACAGCCGTCGGGGCAATAATCGTTGAAAAATGTGTCGCATATTCACCAGACTTAAAGCCTATCCCAGTGGGCGTAATTGTTGCAGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCG  >  NC_000913/2689355‑2689597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: