Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,823,930	G→A	S139S (AGC→AGT)	pncC ←	nicotinamide‑nucleotide amidohydrolase, NMN amidohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,823,930	0	G	A	100.0%	127.7 / NA	37	S139S (AGC→AGT)	pncC	nicotinamide‑nucleotide amidohydrolase, NMN amidohydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/17);  total (20/17)

TCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGACACG  >  NC_000913/2823804‑2824050                                                                                                                               |                                                                                                                         tCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTcc                                                                                                              >  1:93235/1‑139 (MQ=255)           cTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTaa                                                                                                    <  2:271029/139‑1 (MQ=255)                        tataCTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACcgcg                                                                                       <  2:93235/139‑1 (MQ=255)                               tATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGc                                                                                    <  2:288639/135‑1 (MQ=255)                               tATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGc                                                                                    >  1:288639/1‑135 (MQ=255)                               tATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTg                                                                                >  1:134361/1‑139 (MQ=255)                                 tGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGc                                                                              >  1:253912/1‑139 (MQ=255)                                    tCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCaaa                                                                           >  2:341645/1‑139 (MQ=255)                                      aTACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCAt                                                                                                                 >  2:222263/1‑99 (MQ=255)                                      aTACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCAt                                                                                                                 <  1:222263/99‑1 (MQ=255)                                       tACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGc                                                                        >  2:471315/1‑139 (MQ=255)                                         cAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCACTGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCaa                                                                      >  2:83166/1‑139 (MQ=255)                                         cAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCaa                                                                      >  1:181504/1‑139 (MQ=255)                                           gTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCCGGGGCAAAACCaacc                                                                    >  1:406045/1‑137 (MQ=255)                                            tATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTg                                                                                                                       <  1:207537/87‑1 (MQ=255)                                            tATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTg                                                                                                                       >  2:207537/1‑87 (MQ=255)                                            tATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAAcc                                                                   >  1:7898/1‑139 (MQ=255)                                               cAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGa                                                                <  1:471315/139‑1 (MQ=255)                                               cAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGa                                                                <  2:7898/139‑1 (MQ=255)                                                        tGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGa                                                       >  2:476516/1‑139 (MQ=255)                                                        tGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGa                                                       >  2:47565/1‑139 (MQ=255)                                                                     gCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTc                                          >  1:186660/1‑139 (MQ=255)                                                                          aaGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTgc                                     >  2:236983/1‑139 (MQ=255)                                                                                       cATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCaa                                                                            <  1:12662/87‑1 (MQ=255)                                                                                       cATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCaa                                                                            >  2:12662/1‑87 (MQ=255)                                                                                       cATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATccggccc                        <  2:186660/139‑1 (MQ=255)                                                                                        aTACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATccggcccg                       <  1:341645/139‑1 (MQ=255)                                                                                             cAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGAt                  <  2:253912/139‑1 (MQ=255)                                                                                             cAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGAt                  <  1:83166/139‑1 (MQ=255)                                                                                               gTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATAc                <  2:134361/139‑1 (MQ=255)                                                                                                 aGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCa              >  2:263701/1‑139 (MQ=255)                                                                                                 aGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCa              <  2:181504/139‑1 (MQ=255)                                                                                                   ccTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGc                                                                        <  2:51078/79‑1 (MQ=255)                                                                                                   ccTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGc                                                                        >  1:51078/1‑79 (MQ=255)                                                                                                            gCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACa                                                     >  1:141405/1‑89 (MQ=255)                                                                                                            gCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACa                                                     <  2:141405/89‑1 (MQ=255)                                                                                                             cACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGACACg  <  1:476516/139‑1 (MQ=255)                                                                                                                               |                                                                                                                         TCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGACACG  >  NC_000913/2823804‑2824050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑