Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,823,930 G→A S139S (AGC→AGT pncC ← nicotinamide‑nucleotide amidohydrolase, NMN amidohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,823,9300GA100.0% 127.7 / NA 37S139S (AGC→AGTpncCnicotinamide‑nucleotide amidohydrolase, NMN amidohydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/17);  total (20/17)

TCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGACACG  >  NC_000913/2823804‑2824050
                                                                                                                              |                                                                                                                        
tCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTcc                                                                                                              >  1:93235/1‑139 (MQ=255)
          cTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTaa                                                                                                    <  2:271029/139‑1 (MQ=255)
                       tataCTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACcgcg                                                                                       <  2:93235/139‑1 (MQ=255)
                              tATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGc                                                                                    <  2:288639/135‑1 (MQ=255)
                              tATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGc                                                                                    >  1:288639/1‑135 (MQ=255)
                              tATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTg                                                                                >  1:134361/1‑139 (MQ=255)
                                tGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGc                                                                              >  1:253912/1‑139 (MQ=255)
                                   tCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCaaa                                                                           >  2:341645/1‑139 (MQ=255)
                                     aTACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCAt                                                                                                                 >  2:222263/1‑99 (MQ=255)
                                     aTACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCAt                                                                                                                 <  1:222263/99‑1 (MQ=255)
                                      tACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGc                                                                        >  2:471315/1‑139 (MQ=255)
                                        cAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCACTGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCaa                                                                      >  2:83166/1‑139 (MQ=255)
                                        cAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCaa                                                                      >  1:181504/1‑139 (MQ=255)
                                          gTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCCGGGGCAAAACCaacc                                                                    >  1:406045/1‑137 (MQ=255)
                                           tATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTg                                                                                                                       <  1:207537/87‑1 (MQ=255)
                                           tATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTg                                                                                                                       >  2:207537/1‑87 (MQ=255)
                                           tATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAAcc                                                                   >  1:7898/1‑139 (MQ=255)
                                              cAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGa                                                                <  1:471315/139‑1 (MQ=255)
                                              cAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGa                                                                <  2:7898/139‑1 (MQ=255)
                                                       tGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGa                                                       >  2:476516/1‑139 (MQ=255)
                                                       tGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGa                                                       >  2:47565/1‑139 (MQ=255)
                                                                    gCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTc                                          >  1:186660/1‑139 (MQ=255)
                                                                         aaGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTgc                                     >  2:236983/1‑139 (MQ=255)
                                                                                      cATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCaa                                                                            <  1:12662/87‑1 (MQ=255)
                                                                                      cATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCaa                                                                            >  2:12662/1‑87 (MQ=255)
                                                                                      cATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATccggccc                        <  2:186660/139‑1 (MQ=255)
                                                                                       aTACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATccggcccg                       <  1:341645/139‑1 (MQ=255)
                                                                                            cAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGAt                  <  2:253912/139‑1 (MQ=255)
                                                                                            cAGTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGAt                  <  1:83166/139‑1 (MQ=255)
                                                                                              gTAGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATAc                <  2:134361/139‑1 (MQ=255)
                                                                                                aGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCa              >  2:263701/1‑139 (MQ=255)
                                                                                                aGCCTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCa              <  2:181504/139‑1 (MQ=255)
                                                                                                  ccTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGc                                                                        <  2:51078/79‑1 (MQ=255)
                                                                                                  ccTGACGACGCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGc                                                                        >  1:51078/1‑79 (MQ=255)
                                                                                                           gCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACa                                                     >  1:141405/1‑89 (MQ=255)
                                                                                                           gCACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACa                                                     <  2:141405/89‑1 (MQ=255)
                                                                                                            cACCGCATCACGGTCGCCACTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGACACg  <  1:476516/139‑1 (MQ=255)
                                                                                                                              |                                                                                                                        
TCAATATGTTCTGTTGAAGCAATTATACTGTATGCTCATACAGTATCAAGTGTTTTGTAGAAATTGTTGCCACAAGGTCTGCAATGCATACGCAGTAGCCTGACGACGCACCGCATCACGGTCGCCGCTGAAGCATTCCCGCCGGGTAATGCCTTCACCGCGGGCAGTGGCAAAAGCAAACCAGACGGTGCCGACAGGCTTCTCTTCACTGCCGCCATCCGGCCCGGCGATACCACTAATAGACACG  >  NC_000913/2823804‑2824050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: