Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,139,583	G→A	R286H (CGT→CAT)	yghU →	putative S‑transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,139,583	0	G	A	100.0%	94.8 / NA	28	R286H (CGT→CAT)	yghU	putative S‑transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/15);  total (13/15)

CGCTGGGCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCA  >  NC_000913/3139447‑3139718                                                                                                                                         |                                                                                                                                        cGCTGGGCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATc                                                                                                                                       <  2:350650/139‑1 (MQ=255)       gCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGt                                                                                                                                 <  1:35770/139‑1 (MQ=255)             gaagTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGt                                                                                                                           <  1:448494/139‑1 (MQ=255)               agTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTCCGAGACGAATACGGAAGATAAGCATCAGGGGTTAGGGTTg                                                                                                                         <  2:305883/139‑1 (MQ=255)                gTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTgg                                                                                                                        >  1:433045/1‑139 (MQ=255)                                         gTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAgg                                                                                               <  1:150749/139‑1 (MQ=255)                                                       aaCCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAg                                                                                                                              >  2:382629/1‑94 (MQ=255)                                                       aaCCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAg                                                                                                                              <  1:382629/94‑1 (MQ=255)                                                          cGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCAc                                                                              >  2:170089/1‑139 (MQ=255)                                                            cACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGACAAGGCGTTCAcgc                                                                            >  2:159978/1‑139 (MQ=255)                                                                         cTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTg                                                                                                                         <  2:231807/81‑1 (MQ=255)                                                                         cTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTg                                                                                                                         >  1:231807/1‑81 (MQ=255)                                                                         cTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAt                                                               >  2:474663/1‑139 (MQ=255)                                                                                 gCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGcaca                                                       >  2:17420/1‑139 (MQ=255)                                                                                  cAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAGTACGGAAGATAAGCATCAGGGGTa                                                                                                                                >  2:219994/1‑65 (MQ=255)                                                                                  cAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAGTACGGAAGATAAGCATCAGGGGTa                                                                                                                                <  1:219994/65‑1 (MQ=255)                                                                                      tGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATc                                                                                                                                       >  1:386232/1‑54 (MQ=255)                                                                                      tGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATc                                                                                                                                       <  2:386232/54‑1 (MQ=255)                                                                                          tGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGAt                                              >  2:214706/1‑139 (MQ=255)                                                                                              cgcCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGa                                          >  1:204438/1‑139 (MQ=255)                                                                                                    gACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGc                                    <  1:214706/139‑1 (MQ=255)                                                                                                         cAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCTTCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGt                               <  1:474663/139‑1 (MQ=255)                                                                                                              aTTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAg                                                                                                <  2:229080/69‑1 (MQ=255)                                                                                                              aTTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAg                                                                                                >  1:229080/1‑69 (MQ=255)                                                                                                              aTTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTAt                          <  2:204438/139‑1 (MQ=255)                                                                                                              aTTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGACAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTAt                          <  1:159978/139‑1 (MQ=255)                                                                                                                          aTACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGaa              >  1:81520/1‑139 (MQ=255)                                                                                                                                      aGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAAtcatca  <  2:81520/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        CGCTGGGCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCA  >  NC_000913/3139447‑3139718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑