Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,256,941 G→A A357V (GCC→GTC)  yhaO ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,256,9410GA100.0% 101.5 / NA 30A357V (GCC→GTC) yhaOputative transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/19);  total (11/19)

CTACCATGCCGAAAATCGGGCTACAGATAGAGGTGAAGCTCAACACCGGTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGGCAGGCATCTTGCGACGCAGGATGTTCATTACGATCCCTTGCGTTGCTTCGCGAAAGCCTAAATAGACGCCAAAGAATGCAGTCATGACAGCAAAGATATTGAGAATGACGCTGACCACTTTCAC  >  NC_000913/3256807‑3257065
                                                                                                                                      |                                                                                                                            
cTACCATGCCGAAAATCGGGCTACAGATAGAGGTGAAGCTCAACACCGGTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGCTTTTCGTTAATCTTCTCgacagg                                                                                                                          >  1:76412/1‑139 (MQ=255)
         cGAAAATCGGGCTACAGATAGAGGTGAAGCTCAACACCGGTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCg                                                                                                                 >  1:394435/1‑139 (MQ=255)
           aaaaTCGGGCTACAGATAGAGGTGAAGCTCAACACCGGTGCGTTCAGTACGATGGCTCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGAc                                                                                                               <  2:394435/139‑1 (MQ=255)
           aaaaTCGGGCTACAGATAGAGGTGAAGCTCAACACCGGTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGAc                                                                                                               >  2:359509/1‑139 (MQ=255)
             aaTCGGGCTACAGATAGAGGTGAAGCTCAACACCGGTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGc                                                                                                             <  2:206824/139‑1 (MQ=255)
                cGGGCTACAGATAGAGGTGAAGCTCAACACCGGTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAgg                                                                                                          <  2:83643/139‑1 (MQ=255)
                cGGGCTACAGATAGAGGTGAAGCTCAACACCGGTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAgg                                                                                                          <  1:317430/139‑1 (MQ=255)
                cGGGCTACAGATAGAGGTGAAGCTCAACACCGGTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAgg                                                                                                          <  2:487869/139‑1 (MQ=255)
                 gggCTACAGATAGAGGTGAAGCTCAACACCGGTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGa                                                                                                         <  2:415532/139‑1 (MQ=255)
                                 tGAAGCTCAACCCCGGTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTACGATccc                                                                                         >  1:27756/1‑139 (MQ=255)
                                   aaGCTCAACACCGGTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTACGATCCCtt                                                                                       >  1:102745/1‑139 (MQ=255)
                                      cTCAACACCGGTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTACGATCCCTTGCg                                                                                    <  1:104752/139‑1 (MQ=255)
                                       tCAACCCCGGTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTACGATCCCTTGCGt                                                                                   <  2:27756/139‑1 (MQ=255)
                                         aaCACCGGTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTACGATCCCTTGCGTTg                                                                                 >  2:425742/1‑139 (MQ=255)
                                                gTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTACGATCCCTTGCGTTGCTTcgcg                                                                          <  2:431089/139‑1 (MQ=255)
                                                gTGAGTTCAGTACGATGGCGCTCCATGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTACGATCCCTTGCGTTGCTTcgcg                                                                          <  2:323781/139‑1 (MQ=255)
                                                     ttCAGTACCATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTACGATCCCTTGCGTTGATTCGCGAAAGc                                                                     >  2:326384/1‑139 (MQ=255)
                                                              aTGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTACGATCCCTTGCGTTGCTTCGCGAAAGCCTAAATAGa                                                            <  1:345496/139‑1 (MQ=255)
                                                               tGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCa                                                                                                   >  2:345510/1‑99 (MQ=255)
                                                               tGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCa                                                                                                   <  1:345510/99‑1 (MQ=255)
                                                                  cgcTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTAc                                                                                               <  2:60516/100‑1 (MQ=255)
                                                                  cgcTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTAc                                                                                               >  1:60516/1‑100 (MQ=255)
                                                                               cAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGtt                                                                                                     >  1:362968/1‑81 (MQ=255)
                                                                               cAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGtt                                                                                                     <  2:362968/81‑1 (MQ=255)
                                                                                        gAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTACGATCCCTTGCGTTGCTTCGCGAAAGCCTAAATAGACGCCAAAGAATGCAGTCATGACAGCa                                  <  1:359509/139‑1 (MQ=255)
                                                                                             tCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTACGATCCCTTGCGTTGCTTCGCGAAAGCCTAAATAGACGCCAAAGAATGCAGTCATGACAGCAAAGat                             <  1:380905/139‑1 (MQ=255)
                                                                                                tgatgCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTACGATCCCTTGCGTTGCTTCGCGAAAGCCTAAATAGACGCCAAAGAATGCAGTCATGACAGCAAAGATAtt                          <  2:102745/139‑1 (MQ=255)
                                                                                                             aaCGAGATTTTCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTACGATCCCTTGCGTTGCTTCGCGAAAGCCTAAATAGACGCCAAAGAATGCAGTCATGACAGCAAAGATATTGAGAATGACGCTg             >  2:419484/1‑139 (MQ=255)
                                                                                                                       tCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGAt                                                                                                        >  1:179772/1‑38 (MQ=255)
                                                                                                                       tCGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGAt                                                                                                        <  2:179772/38‑1 (MQ=255)
                                                                                                                        cGTTAATCTTCTCGACAGGCATCTTGCGACGCAGGATGTTCATTACGATCCCTTGCGTTGCTTCGCGAAAGCCTAAATAGACGCCAAAGAATGCAGTCATGACAGCAAAGATATTGAGAATGACGCTGACCACTTTCAc  <  1:419484/139‑1 (MQ=255)
                                                                                                                                      |                                                                                                                            
CTACCATGCCGAAAATCGGGCTACAGATAGAGGTGAAGCTCAACACCGGTGCGTTCAGTACGATGGCGCTCCAGGCCAGCAAAATGGCGAAAATCATGATGCCGCGCTGAACGAGATTTTCGTTAATCTTCTCGGCAGGCATCTTGCGACGCAGGATGTTCATTACGATCCCTTGCGTTGCTTCGCGAAAGCCTAAATAGACGCCAAAGAATGCAGTCATGACAGCAAAGATATTGAGAATGACGCTGACCACTTTCAC  >  NC_000913/3256807‑3257065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: