Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,315,163	G→A	G284G (GGC→GGT)	infB ←	translation initiation factor IF‑2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,315,163	0	G	A	100.0%	40.7 / NA	14	G284G (GGC→GGT)	infB	translation initiation factor IF‑2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/7);  total (7/7)

CGCCATCTTGTTCGCCAGTTCGCCAACGGTGATAGTTTCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAGCCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCG  >  NC_000913/3315037‑3315286                                                                                                                               |                                                                                                                            cGCCATCTTGTTCGCCAGTTCGCCAACGGTGATAGTTTCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAGCCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCACCACGTActgct                                                                                                                 <  1:95910/139‑1 (MQ=255)                                      tCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAGCCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCACCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCgt                                                                            >  1:426737/1‑139 (MQ=255)                                      tCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAGCCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCACCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCgt                                                                            >  2:343460/1‑139 (MQ=255)                                               acaACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAGCCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCACCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGc                                                                   <  1:91543/139‑1 (MQ=255)                                                             aaCGGCCTGAGCAGGCTTCTGGAAGCCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCACCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCt                                                                                         >  1:172503/1‑103 (MQ=255)                                                             aaCGGCCTGAGCAGGCTTCTGGAAGCCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCACCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCt                                                                                         <  2:172503/103‑1 (MQ=255)                                                                   cTGAGCAGGCTTCTGGAAGCCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCACCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGACGCgctgct                                               >  1:252194/1‑139 (MQ=255)                                                                                tGGAAGCCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCACCACGTACTGCTGcgcg                                                                                                            >  1:312057/1‑65 (MQ=255)                                                                                tGGAAGCCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCACCACGTACTGCTGcgcg                                                                                                            <  2:312057/65‑1 (MQ=255)                                                                                                           ttACGTTTTCCGCCTTTACCACCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGAtt                                                                                  >  1:135373/1‑64 (MQ=255)                                                                                                           ttACGTTTTCCGCCTTTACCACCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGAtt                                                                                  <  2:135373/64‑1 (MQ=255)                                                                                                           ttACGTTTTCCGCCTTTACCACCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCAc       >  1:452652/1‑139 (MQ=255)                                                                                                             aCGTTTTCCGCCTTTACCACCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGa     <  2:252194/139‑1 (MQ=255)                                                                                                                ttttCCGCCTTTACCACCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCg  <  2:227726/139‑1 (MQ=255)                                                                                                                               |                                                                                                                            CGCCATCTTGTTCGCCAGTTCGCCAACGGTGATAGTTTCGCCGATCACAACGTCACGGTTAACGGCCTGAGCAGGCTTCTGGAAGCCTTGCTGCAGCGAAGAACCTTTACGTTTTCCGCCTTTACCGCCACGTACTGCTGCGCGTGCTTCTTCACGATCAGCTTTTGATTCAGCGTGTTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCG  >  NC_000913/3315037‑3315286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑