Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,946,742	C→T	noncoding (43/120 nt)	rrfC →	5S ribosomal RNA of rrnC operon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,946,742	0	C	T	100.0%	22.5 / NA	12	noncoding (43/120 nt)	rrfC	5S ribosomal RNA of rrnC operon
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/7);  total (5/7)

CGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGT  >  NC_000913/3946615‑3946802                                                                                                                                |                                                             cGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCTGAACTCAGAAg                                                   >  2:197666/1‑139 (MQ=21)        gTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCTGAACTCAGAAGTGAAACg                                            <  1:273400/139‑1 (MQ=18)                                  aGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCTGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGt                  <  2:368448/139‑1 (MQ=17)                                      tGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCTGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTcc              >  1:302944/1‑139 (MQ=17)                                           cAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCTGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATg         <  1:305803/139‑1 (MQ=18)                                              aTTAAATCAGAACGCAGAAGCGGTCTGATGAAACACAATTTGCCTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCTGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCga      <  2:302944/139‑1 (MQ=17)                                                 aaaTCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCTGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGagag   <  2:315615/139‑1 (MQ=18)                                                  aaTCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCTGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGt  >  2:147066/1‑139 (MQ=17)                                                        aaCGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCTGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATg         >  1:84656/1‑126 (MQ=18)                                                        aaCGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCTGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATg         <  2:84656/126‑1 (MQ=18)                                                          cGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCTGAACTCAGAAGTGAAACGCCGTa                                       >  1:377196/1‑94 (MQ=21)                                                          cGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCTGAACTCAGAAGTGAAACGCCGTa                                       <  2:377196/94‑1 (MQ=21)                                                                                                                                |                                                             CGAAGATGTTTTGGCGGATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATGAAACAGAATTTGCCTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGT  >  NC_000913/3946615‑3946802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑