Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,078,169	C→T	R241C (CGT→TGT)	yiiD →	GNAT family putative N‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,078,169	0	C	T	100.0%	40.2 / NA	13	R241C (CGT→TGT)	yiiD	GNAT family putative N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/5);  total (8/5)

GGCAAAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGATGCTGCGTGAACGCCACCTCGGCGGAACGATTATTCTTGCGGATGCGCATATCCGCTACAGCAAGCCGATTAGCGGTAAACCTCATGCGGTAGCCGACCTCGGTGCCTTAAGCGGCGATCTCGACCGTCTGGCGC  >  NC_000913/4078047‑4078298                                                                                                                           |                                                                                                                                  ggCAAAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGATGCTGTGTGAACGCCACCTcgg                                                                                                                   >  1:344507/1‑139 (MQ=255) ggCAAAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGATGCTGTGTGAACGCCACCTcgg                                                                                                                   >  2:229601/1‑139 (MQ=255)    aaaaaTTTATCACTACCATTCGAGAAACCGGCAATCAGAAACCGCACCATACGCTGTTTGCCTGGAGTTGATTCTCACTGGCGACGGGCACTGGTTGGGGACTTATCTGGCTGATGCTGTGTGAACGCCACCTcggcgg                                                                                                                <  2:356841/139‑1 (MQ=255)         tttATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGATGCTGTGTGAACGCCACCTCGGCGGAACGa                                                                                                           <  1:229601/139‑1 (MQ=255)                                  gCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGATGCTGTGTGAACGCCACCTCGGCGGAACGATTATTCTTGCGGATGCGCATATCCg                                                                                  >  1:76204/1‑139 (MQ=255)                                                   cATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGATGCTGTGTGAACGCCACCTCGGCGGAACGATTATTCTTGCGGATGCGCATATCCGCTACAGCAAGCCGATTa                                                                 <  2:76204/139‑1 (MQ=255)                                                                         tATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGATGCTGTGTGAACGCCACCTCGGCGGAACGATTATTCTTGCGGATGCGCata                                                                                      >  1:287471/1‑96 (MQ=255)                                                                         tATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGATGCTGTGTGAACGCCACCTCGGCGGAACGATTATTCTTGCGGATGCGCata                                                                                      <  2:287471/96‑1 (MQ=255)                                                                                              cTGGTTGGGGACTTATCTGGCTGATGCTGTGTGAACGCCACCTCGGCGGAACGATTATTCTTGCGGATGCGCATATCCGCTACAGCAAGCCGATTAGCGGTAAACCTCATGCGGTAGCCGACCTCGGTGCCTTAAgcgg                      >  2:233574/1‑139 (MQ=255)                                                                                                       gACTTATCTGGCTGATGCTGTGTGAACGCCACCTCGGCGGAACGATTATTCTTGCGGATGCGCATATCCGCTACAGCAAGCCGATTAGCGGTAAACCTCATGCGGTAGCCGACCTCGGTGCCTTAAGCGGCGATCTCGa             >  1:421927/1‑139 (MQ=255)                                                                                                            aTCTGGCTGATGCTGTGTGAACGCCACCTCGGCGGAACGATTATTCTTGCgg                                                                                               <  2:309570/52‑1 (MQ=255)                                                                                                            aTCTGGCTGATGCTGTGTGAACGCCACCTCGGCGGAACGATAATTCTTGCgc                                                                                               >  1:309570/1‑51 (MQ=255)                                                                                                                  cTGATGCTGTGTGAACGCCACCTCGGCGGAACGATTATTCTTGCGGATGCGCATATCCGCTACAGCAAGCCGATTAGCGGTAAACCTCATGCGGTAGCCGACCTCGGTGCCTTAAGCGGCGATCTCGACCGTCTGgcgc  >  2:483433/1‑139 (MQ=255)                                                                                                                      tgctgtGTGAACGCCACCTCGGCGGAACGATTATTCTTGCGGATGCGCATATCCGCTACAGCAAGCCGATTAGCGGTAAACCTCATGCGGTAGCCGACCTCGGTGCCTTAAgcg                       <  1:414309/114‑1 (MQ=255)                                                                                                                      tgctgtGTGAACGCCACCTCGGCGGAACGATTATTCTTGCGGATGCGCATATCCGCTACAGCAAGCCGATTAGCGGTAAACCTCATGCGGTAGCCGACCTCGGTGCCTTAAgcg                       >  2:414309/1‑114 (MQ=255)                                                                                                                           |                                                                                                                                  GGCAAAAATTTATCACTACCATGCCAGAAACCGGCAATCAGAATCCGCACCATACGCTGTTTGCCGGGAGTTTATTCTCACTGGCGACGCTCACTGGTTGGGGACTTATCTGGCTGATGCTGCGTGAACGCCACCTCGGCGGAACGATTATTCTTGCGGATGCGCATATCCGCTACAGCAAGCCGATTAGCGGTAAACCTCATGCGGTAGCCGACCTCGGTGCCTTAAGCGGCGATCTCGACCGTCTGGCGC  >  NC_000913/4078047‑4078298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑