Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,160,278	G→A	S171S (AGC→AGT)	sthA ←	pyridine nucleotide transhydrogenase, soluble

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,160,278	0	G	A	100.0%	92.0 / NA	27	S171S (AGC→AGT)	sthA	pyridine nucleotide transhydrogenase, soluble
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/14);  total (13/14)

CCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGTTAGTGTTTCAACGCTGCC  >  NC_000913/4160184‑4160397                                                                                               |                                                                                                                        ccACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATc                                                                             >  1:253061/1‑139 (MQ=255)              cATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGga                                                                <  2:128384/139‑1 (MQ=255)               aTACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGaa                                                                                >  2:41601/1‑122 (MQ=255)               aTACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGaa                                                                                <  1:41601/122‑1 (MQ=255)               aTACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCaa                                                                           <  1:165258/127‑1 (MQ=255)               aTACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCaa                                                                           >  2:165258/1‑127 (MQ=255)                 aCCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGgatga                                                             >  2:88945/1‑139 (MQ=255)                   cgcgGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGata                                                           >  2:261853/1‑139 (MQ=255)                     cgGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATg                                                         >  1:50822/1‑139 (MQ=255)                     cgGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATg                                                         >  1:202890/1‑139 (MQ=255)                       gAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGa                                                       <  2:50822/139‑1 (MQ=255)                                                aTCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAg                                                                                                             >  1:425133/1‑60 (MQ=255)                                                aTCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAg                                                                                                             <  2:425133/60‑1 (MQ=255)                                                     tCCAGCACCATATATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACAGCTGTTGGATGAGATGGACGAGAGCCGCAGGCAATAACAAATTTTTCa                         <  1:261853/139‑1 (MQ=255)                                                          cACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGt                    <  1:88945/139‑1 (MQ=255)                                                          cACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGt                    <  2:202890/139‑1 (MQ=255)                                                             cATAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGTTAg                 <  2:333902/139‑1 (MQ=255)                                                               tAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACAt                                                                        >  1:384772/1‑82 (MQ=255)                                                               tAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACAt                                                                        <  2:384772/82‑1 (MQ=255)                                                               tAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGc                                             >  1:182540/1‑109 (MQ=255)                                                               tAGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGc                                             <  2:182540/109‑1 (MQ=255)                                                                aGATAAGTACATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGTTAgtgt              <  1:88167/139‑1 (MQ=255)                                                                         cATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTc                                                                                                     >  2:218326/1‑43 (MQ=255)                                                                         cATGGCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTc                                                                                                     <  1:218326/43‑1 (MQ=255)                                                                            ggCGCGGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGTTAGTGTTTCAACGCTGcc  >  2:166027/1‑139 (MQ=255)                                                                              cgcgGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACga                                                    <  2:198489/87‑1 (MQ=255)                                                                              cgcgGTTCGTGGTGCATACTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACga                                                    >  1:198489/1‑87 (MQ=255)                                                                                               |                                                                                                                        CCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGTTAGTGTTTCAACGCTGCC  >  NC_000913/4160184‑4160397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑