Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,385,906 T→C L68P (CTC→CCC)  yjeO → inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,385,9060TC100.0% 63.8 / NA 18L68P (CTC→CCC) yjeOinner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/8);  total (10/8)

ATGGACGCGGGATTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCTCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCAGTTTTGTTTGTGAGCCGGATTGGTTCATCCGGCACA  >  NC_000913/4385801‑4386041
                                                                                                         |                                                                                                                                       
atGGACGCGGGATTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCt                                                                                                         <  1:485176/138‑1 (MQ=255)
atGGACGCGGGATTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCt                                                                                                         >  2:485176/1‑138 (MQ=255)
  ggACGCGGGATTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGt                                                                                                      <  2:386305/139‑1 (MQ=255)
       cgGGATTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGTTTATg                                                                                                 >  2:27407/1‑139 (MQ=255)
                                    tGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTa                                                                    <  1:366815/139‑1 (MQ=255)
                                        tctcGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGTTTATGTACGCCACCGCGCTGACTATTTTCGGCTACTgg                                                                >  2:428022/1‑139 (MQ=255)
                                          tcGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCt                                                              >  1:386818/1‑139 (MQ=255)
                                                  gaCGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGaaaaaaa                                                                                                                >  1:438443/1‑81 (MQ=255)
                                                  gaCGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGaaaaaaa                                                                                                                <  2:438443/81‑1 (MQ=255)
                                                   atgATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGtt                                                     >  1:87964/3‑139 (MQ=255)
                                                                tGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTAt                                        <  2:87964/139‑1 (MQ=255)
                                                                  ttGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGttt                                                                                                    >  2:140263/1‑77 (MQ=255)
                                                                  ttGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGttt                                                                                                    <  1:140263/77‑1 (MQ=255)
                                                                    ggTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCAGt                                    >  2:201713/1‑139 (MQ=255)
                                                                                    tACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGTTTATGTACGCCACcgcg                                                                                     >  1:61928/1‑74 (MQ=255)
                                                                                    tACCGCTCTTTTTCCCTTTTCCCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGTTTATGTACGCCACcgcg                                                                                     <  2:61928/74‑1 (MQ=255)
                                                                                                    ttttCCCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCAGTTTTGTTTGTGAGCCGGATTGGTTCATCCGGca    >  1:268128/1‑139 (MQ=255)
                                                                                                      ttCCCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCAGTTTTGTTTGTGAGCCGGATTGGTTCATCCGGcaca  <  2:268128/139‑1 (MQ=255)
                                                                                                         |                                                                                                                                       
ATGGACGCGGGATTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCTCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCAGTTTTGTTTGTGAGCCGGATTGGTTCATCCGGCACA  >  NC_000913/4385801‑4386041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: