| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,442,061 | A→G | V39A (GTA→GCA) | msrA ← | methionine sulfoxide reductase A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,442,061 | 0 | A | G | 96.2% | 85.7 / ‑6.5 | 26 | V39A (GTA→GCA) | msrA | methionine sulfoxide reductase A |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (12/13); minor base T (0/1); total (12/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGGGTGTTACGTCCAGGCAGGGCATCGGCGGGGGAAACCAGATGCTTTTTATCAAATAAACTCA > NC_000913/4441926‑4442175 | cGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTGCAt < 2:81856/139‑1 (MQ=255) aaGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTGCATTGGt < 1:392496/139‑1 (MQ=255) aTTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGATCAGACGCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTGCATTGGTCATTGAg < 1:358550/139‑1 (MQ=255) ttGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTGCATTGGTCATTGAGTg < 2:72465/139‑1 (MQ=255) tGCTGTAAACGCCGGGTAACTGCCAGAACAGACCCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTGCATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCAt > 2:66804/1‑139 (MQ=255) cTGTAAACGCCGGGTAACTGCCAGAACAGACGCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTGCATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCg < 1:275666/139‑1 (MQ=255) tAAACGCCGGGTAACTGCCAGAACAGACTCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTGCATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCgggg > 1:363305/1‑139 (MQ=255) gCCGGGTAACTGCCAGAACAGACGCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTGCATTGGTCATTGAGTGACCGt > 2:42863/1‑101 (MQ=255) gCCGGGTAACTGCCAGAACAGACGCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTGCATTGGTCATTGAGTGACCGt < 1:42863/101‑1 (MQ=255) cGGGTAACTGCCAGAACAGACGCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTGCATTGGTCATTg < 2:457241/90‑1 (MQ=255) cGGGTAACTGCCAGAACAGACGCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTGCATTGGTCATTg > 1:457241/1‑90 (MQ=255) aCTGCCAGAACAGACGCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTTCATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGGGTGTTACGTCcagg < 2:363305/139‑1 (MQ=255) tGCCAGAACAGACGCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTGCATTGGTCATTGAGTGACCGTTGAcc < 2:120472/96‑1 (MQ=255) tGCCAGAACAGACGCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTGCATTGGTCATTGAGTGACCGTTGAcc > 1:120472/1‑96 (MQ=255) cccAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTGCATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGGGTGTTACGTCCAGGCAGGGCATCGGCGGGGGAAACCAg > 2:130614/1‑139 (MQ=255) aaaCAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTGCATTGGTCATTGAGTGACCGTTGACCGCATg < 2:399288/74‑1 (MQ=255) aaaCAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTGCATTGGTCATTGAGTGACCGTTGACCGCATg > 1:399288/1‑74 (MQ=255) acCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTGCATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGGGTGTTACGTCCAGGCAGGGCATCGGCGGGGGAAACCAGATGCTTTTTa > 2:332904/1‑139 (MQ=255) acCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTGCATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGGGTGTTACGTCCAGGCAGGGCATCGGCGGGGGAAACCAGATGCTTTTTa > 2:168711/1‑139 (MQ=255) cccATCGCAAAAATGGCAATCTCCATTCCGTCAGGTGCATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGGGTGTTACGTCCAGGCAGGGCATCGGCGGGGGAAACCAGATGCTTTTTAt < 1:168711/139‑1 (MQ=255) cGCAAAAATGGCAATCTCCATTCCGTCAGGTGCATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGGGCGTTACGTCCAGGCAGGGCATCGGCGGGGGAAACCAGATGCTTTTTATCAAAt > 2:195026/1‑139 (MQ=255) aTGGCAATCTCCATTCCGTCAGGTGCATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGGGTGTTACGTCCAGGCAGGGCATCGGCGGGGGAAACCAGATGCTTTTTATCAAATAAACTCa < 1:426669/139‑1 (MQ=255) aaTCTCCATTCCGTCAGGTGCATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGGGTGTTACGTCcagg < 2:271425/87‑1 (MQ=255) aaTCTCCATTCCGTCAGGTGCATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGGGTGTTACGTCcagg > 1:271425/1‑87 (MQ=255) tCAGGTGCATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGGGTGTTACGTCCAGGCAGGGCATCGGCGGGGGAAACCAGATGCTTTTTATCAAATAAACTCa < 1:303365/121‑1 (MQ=255) tCAGGTGCATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGGGTGTTACGTCCAGGCAGGGCATCGGCGGGGGAAACCAGATGCTATTTATCAAATAAACTCa > 2:303365/1‑121 (MQ=255) | CGATAAGTCGGATTTGGCGTATAGCCGCCGGTATAGCCTGCGGCGGTGCTGTAAACGCCGGGTAACTGCCAGAACAGACGCTCCACACCCCAGAAACAACCCATCGCAAAAATGGCAATCTCCATTCCGTCAGGTACATTGGTCATTGAGTGACCGTTGACCGCATGCAGCGTGGCTACGGGCATCGGGGTGTTACGTCCAGGCAGGGCATCGGCGGGGGAAACCAGATGCTTTTTATCAAATAAACTCA > NC_000913/4441926‑4442175 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |