Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,595,843	A→G	R3R (CGT→CGC)	lgoR ←	putative transcriptional activator for L‑galactonate catabolism

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,595,843	0	A	G	100.0%	54.3 / NA	15	R3R (CGT→CGC)	lgoR	putative transcriptional activator for L‑galactonate catabolism
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/7);  total (8/7)

ATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTCCTCCTTGTGAGTCCATTAAAACCCGATTTAAAACATTTTTATGCGTGTGTTTTCACAAAAAAGATTTTCCGTTCCGCTGCCATTTTTCATTTTCATTGATATCTAAGGCA  >  NC_000913/4595711‑4595967                                                                                                                                     |                                                                                                                             atGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATAAGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAGCGACTc                                                                                                                        <  2:49156/139‑1 (MQ=255)                            gtACATTTCCGCCAGCGCACTTCGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAGCGACTCATAttt                                                                                                                  <  1:490788/118‑1 (MQ=255)                            gtACATTTCCGCCAGCGCACTTCGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAGCGACTCATAttt                                                                                                                  >  2:490788/1‑118 (MQ=255)                                  ttCCGCCAGCGCACTTTGCGATGGAAGTGGGGACGGGATATGACCCCGTGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAGCGACTCATATTTTCCTCCTtgt                                                                                                        <  2:318500/122‑1 (MQ=255)                                  ttCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGTGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAGCGACTCATATTTTCCTCCTtgt                                                                                                        >  1:318500/1‑122 (MQ=255)                                                  tGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAGCGACTCATATTTTCCTCCTTGTGAGt                                                                                                    <  1:245585/110‑1 (MQ=255)                                                  tGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAGCGACTCATATTTTCCTCCTTGTGAGt                                                                                                    >  2:245585/1‑110 (MQ=255)                                                                   aGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAGCGACTc                                                                                                                        <  1:422395/73‑1 (MQ=255)                                                                   aGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAGCGACTc                                                                                                                        >  2:422395/1‑73 (MQ=255)                                                                                cgcgCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAGCGACTCATATTTTCCTCCTTGTGAGTCCATTAAAACCCGATTTAAAACATTTTTATGCGTGTGTTTTCACAAAAAAGATTttccg                                         >  1:429343/1‑139 (MQ=255)                                                                                                                 ttGTGGCGTAAATTTTGTGAGCGACTCATATTTTCCTCCTtgt                                                                                                        <  1:416467/43‑1 (MQ=255)                                                                                                                 ttGTGGCGTAAATTTTGTGAGCGACTCATATTTTCCTCCTtgt                                                                                                        >  2:416467/1‑43 (MQ=255)                                                                                                                  tgtgGCGTAAATTTTGTGAGCGACTCATATTTTCCTCCTTGTGAGTCCATTAAAACCCGATTTaaa                                                                                <  1:39603/66‑1 (MQ=255)                                                                                                                  tgtgGCGTAAATTTTGTGAGCGACTCATATTTTCCTCCTTGTGAGTCCATTAAAACCCGATTTaaa                                                                                >  2:39603/1‑66 (MQ=255)                                                                                                                       cGTAAATTTTGTGAGCGACTCATATTTTCCTCCTTGTGAGTCCATTAAAACCCGATTTAAAACATTTTTATGCGTGTGTTTTCACAAAAAAGATTTTCCGTTCCGCTGCCATTTTTCATTTTCATTGATATCTAAGGca  >  1:1414/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                             ATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGGGGAAGGGATATGACCCCGCGCCATATCATCAATGACCTGGTTAATCACATTGTGGCGTAAATTTTGTGAACGACTCATATTTTCCTCCTTGTGAGTCCATTAAAACCCGATTTAAAACATTTTTATGCGTGTGTTTTCACAAAAAAGATTTTCCGTTCCGCTGCCATTTTTCATTTTCATTGATATCTAAGGCA  >  NC_000913/4595711‑4595967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑