Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 521,055 T→C L214L (TTA→CTA)  ybbP → putative ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913521,0550TC100.0% 47.6 / NA 14L214L (TTA→CTA) ybbPputative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/6);  total (8/6)

TGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGTCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTACCTCAGCTTAAACCCGAACAACGCTGGTACGGTCTGGAACAGGACGAAGGCGCGCTGGGGCGATCGATGGAACGCTCGCAACAGTTC  >  NC_000913/520924‑521144
                                                                                                                                   |                                                                                         
tGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGTCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGCTACCTCa                                                                                    >  1:65942/1‑139 (MQ=255)
    tCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGTCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGCTACCTCAGCtt                                                                                >  2:113058/1‑139 (MQ=255)
                                      aaaaCCGGAGCCGTGCAGCCGGGGAGTCGGGTCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGCTACCTCAGCTTAAACCCGAACAACGCTGGTACGGTCTGGAACAgg                                              >  2:62161/1‑139 (MQ=255)
                                                          ggggAGTCGGGTCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGCTACCTCAGCTTAAACCCGAACAACGCTGGTACGGTCTGGAACAGGACGAAGGCGCGCTGGGGcga                          <  2:65942/139‑1 (MQ=255)
                                                                  gggTCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGCTACCTCAGCTTAAACCCGAACAACGCTGGTACGGTCTGGAACAGGACGAAGGCGCGCTGGGGc                            >  1:151619/1‑129 (MQ=255)
                                                                  gggTCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGCTACCTCAGCTTAAACCCGAACAACGCTGGTACGGTCTGGAACAGGACGAAGGCGCGCTGGGGc                            <  2:151619/129‑1 (MQ=255)
                                                                                  taAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGCTACCTCAGCTTGAACCCGAACAACGCTGGTACGGTCTGGAACAGGACGAAGGCGCGCTGGGGCGATCGATGGAACGCTCGCAACAGTTc  >  2:118867/1‑139 (MQ=255)
                                                                                  taAACTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGCTACCTCAGCTTAAACCCGAACAACGCTGGTACGGTCTGGAACAGGACGAAGGCGCGCTGGGGCGATCGATGGAACGCTCGCAACAGTTc  <  2:48314/139‑1 (MQ=255)
                                                                                         ggcggcAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGCTACCTCAGCTTAAACCCGAACAACGCTGGTa                                                            >  1:173390/1‑74 (MQ=255)
                                                                                         ggcggcAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGCTACCTCAGCTTAAACCCGAACAACGCTGGTa                                                            <  2:173390/74‑1 (MQ=255)
                                                                                           cggcAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGCTACCTCAGCTTAAACCCGAACAACGCTGGTa                                                            <  1:455142/72‑1 (MQ=255)
                                                                                           cggcAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGCTACCTCAGCTTAAACCCGAACAACGCTGGTa                                                            >  2:455142/1‑72 (MQ=255)
                                                                                                aCGAGAACCAGCTCGACGGCTATGAGAAATGGTTGCTACCTCAGCTTAAACCCGAACAACGCTGGTACGGTCTGGAACAGGACGAAGGCGCGCTGGGGCGATCGATGGAACGCt             <  1:5014/114‑1 (MQ=255)
                                                                                                aCGAGAACCAGCTCGACGGCTATGAGAAATGGTTGCTACCTCAGCTTAAACCCGAACAACGCTGGTACGGTCTGGAACAGGACGAAGGCGCGCTGGGGCGATCGATGGAACGCt             >  2:5014/1‑114 (MQ=255)
                                                                                                                                   |                                                                                         
TGGCTCCGCGTCTGATGATGAATCTGGCGGATGTCGATAAAACCGGAGCCGTGCAGCCGGGGAGTCGGGTCACCTGGCGTTATAAATTCGGCGGCAACGAGAACCAGCTCGACGGCTATGAGAAATGGTTGTTACCTCAGCTTAAACCCGAACAACGCTGGTACGGTCTGGAACAGGACGAAGGCGCGCTGGGGCGATCGATGGAACGCTCGCAACAGTTC  >  NC_000913/520924‑521144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: