Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 190,778 (G)5→6 intergenic (+179/‑79) rpsB → / → tsf 30S ribosomal subunit protein S2/translation elongation factor EF‑Ts

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913190,7731.G100.0% 119.1 / NA 32intergenic (+174/‑84)rpsB/tsf30S ribosomal subunit protein S2/translation elongation factor EF‑Ts
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (13/19);  total (13/19)

AGAAAGGCGGCAAGCTCGAGAATTCCCGGGAGCTTACATCAGTAAGTGACCGGGATGAGCGAGCGAAGATAACGCATCTGCGGCGCGAAATATGAAGGGGGAGAGCCCTTATAGACCAGGTAGTACACGTTTGGTTAGGGGGCCTGCATATGGCCCCCTTTTTCACTTTTATATCTGTGCGGTTTAATGCCGGGCAGATCACATCTCCGAGGATTTTAGAATGGCTGAAATTACCGCATCCCTGGTAAAAGAGCTGCGTGAGCGTAC  >  NC_000913/190637‑190903
                                                                                                                                         |                                                                                                                                  
aGAAAGGCGGCAAGCTCGAGAATTCCCGGGAGCTTACATCAGTAAGTGACCGGGATGAGCGAGCGAAGATAACGCATCTGCGGCGCGAAATATGAAGGGGGAGAGCCCTTATAGACCAGGTAGTACACGTTTGGTTAgg                                                                                                                                  <  2:62710/139‑1 (MQ=255)
      gcggcAAGCTCGAGAATTCCCGGGAGCTTACATCAGTAAGTGACCGGGATGAGCGAGCGAAGATAACGCATCTGCGGCGCGAAATATGAAGGGGGAGAGCCCTTATAGACCAGGTAGTACACGTTTGGTTAggggggcc                                                                                                                            <  1:737561/139‑4 (MQ=255)
       cggcAAGCTCGAGAATTCCCGGGAGCTTACATCAGTAAGTGACCGGGATGAGCGAGCGAAGATAACGCATCTGCGGCGCGAAATATGAAGGGGGAGAGCCCTTATAGACCAGGTAGTACACGTTTGGTTAggggggc                                                                                                                             >  2:623104/1‑135 (MQ=255)
       cggcAAGCTCGAGAATTCCCGGGAGCTTACATCAGTAAGTGACCGGGATGAGCGAGCGAAGATAACGCATCTGCGGCGCGAAATATGAAGGGGGAGAGCCCTTATAGACCAGGTAGTACACGTTTGGTTAggggggc                                                                                                                             <  1:623104/137‑3 (MQ=255)
                 gagaATTCCCGGGAGCTTACATCAGTAAGTGACCGGGATGAGCGAGCGAAGATAACGCATCTGCGGCGCGAAATATGAAGGGGGAGAGCCCTTATAGACCAGGTAGTACACGTTTGGTTAGGGGGGCCTGCATATGGcc                                                                                                                  <  1:333692/139‑1 (MQ=255)
                         ccGGGAGCTTACATCAGTAAGTGACCGGGATGAGCGAGCGAAGATAACGCATCTGCGGCGCGAAATATGAAGGGGGAGAGCCCTTATAGACCAGGTAGTACACGTTTGGTTAGGGGGGCCTGCATATGGCCCCCttttt                                                                                                          <  1:442587/139‑1 (MQ=255)
                          cGGGAGCTTACATCAGTAAGTGACCGGGATGAGCGAGCGAAGATAACGCATCTGCGGCGCGAAATATGAAGGGGGAGAGCCCTTATAGACCAGGTAGTACACGTTTGGTTAGGGGGGCCTGCATATGGCCCCCTTTTTc                                                                                                         <  1:456467/139‑1 (MQ=255)
                          cGGGAGCTTACATCAGTAAGTGACCGGGATGAGCGAGCGAAGATAACGCATCTGCGGCGCGAAATATGAAGGGGGAGAGCCCTTATAGACCAGGTAGTACACGTTTGGTTAGGGGGGCCTGCATATGGCCCCCTTTTTc                                                                                                         <  1:637508/139‑1 (MQ=255)
                                         gTAAGTGACCGGGATGAGCGAGCGAAGATAACGCATCTGCGGCGCGAAATATGAAGGGGGAGAGCCCTTATAGACCAGGTAGTACACGTTTGGTTAGGGGGGCCTGCATATGGCCCCCTTTTTCACTTTTATATCtgtg                                                                                          <  1:759769/139‑1 (MQ=255)
                                            aGTGACCGGGATGAGCGAGCGAAGATAACGCATCTGCGGCGCGAAATATGAAGGGGGAGAGCCCTTATAGACCAGGTAGTACACGTTTGGTTAGGGGGGCCTGCATATGGCCCCCTTTTTCACTTTTATATCTGTGCgg                                                                                       >  2:652008/1‑139 (MQ=255)
                                             gTGACCGGGATGAGCGAGCGAAGATAACGCATCTGCGGCGCGAAATATGAAGGGGGAGAGCCCTTATAGACCAGGTAGTACACGTTTGGTTAGGGGGGCCTGCATATGGCCCCCTTTTTCACTTTTATATCTGTGCGGt                                                                                      >  2:229033/1‑139 (MQ=255)
                                              tGACCGGGATGAGCGAGCGAAGATAACGCATCTGCGGCGCGAAATATGAAGGGGGAGAGCCCTTATAGACCAGGTAGTACACGTTTGGTTAGGGGGGCCTGCATATGGCCCCCTTTTTCACTTTTATATCTGTGCGGtt                                                                                     <  2:108959/139‑1 (MQ=255)
                                                  cGGGATGAGCGAGCGAAGATAACGCATCTGCGGCGCGAAATATGAAGGGGGAGAGCCCTTATAGACCAGGTAGTACACGTTTGGTTAGGGGGGCCTGCATATGGCCCCCTTTTTc                                                                                                         >  2:362724/1‑115 (MQ=255)
                                                  cGGGATGAGCGAGCGAAGATAACGCATCTGCGGCGCGAAATATGAAGGGGGAGAGCCCTTATAGACCAGGTAGTACACGTTTGGTTAGGGGGGCCTGCATATGGCCCCCTTTTTc                                                                                                         <  1:362724/115‑1 (MQ=255)
                                                   gggATGAGCGAGCGAAGATAACGCATCTGCGGCGCGAAATATGAAGGGGGAGAGCCCTTATAGACCAGGTAGTACACGTTTGGTTAGGGGGGCCTGCATATGGCCCCCTTTTTCACTTTTATATCTGTGCGGTTTAATg                                                                                <  1:652008/139‑1 (MQ=255)
                                                     gATGAGCGAGCGAAGATAACGCATCTGCGGCGCGAAATATGAAGGGGGAGAGCCCTTATAGACCAGGTAGTACACGTTTGGTTAGGGGGGCCTGCATATGGCCCCCTTTTTCACTTTTATATCTGTGCGGTTTAATGcc                                                                              <  1:48973/139‑1 (MQ=255)
                                                          gcgagcgaAGATAACGCATCTGCGGCGCGAAATATGAAGGGGGAGAGCCCTTATAGACCAGGTAGTACACGTTTGGTTAGGGGGGCCTGCATATGGCCCCCTTTTTCACTTTTATATCTGTGCGGTTTAATGCCGGGCa                                                                         <  2:528519/139‑1 (MQ=255)
                                                           cgagcgaAGATAACGCATCTGCGGCGCGAAATATGAAGGGGGAGAGCCCTTATAGACCAGGTAGTACACGTTTGGTTAGGGGGGCCTGCATATGGCCCCCTTTTTCACTTTTATATCTGTGCGGTTTAATGCCGGGCAg                                                                        >  1:142494/1‑139 (MQ=255)
                                                               cgaAGATAACGCATCTGCGGCGCGAAATATGAAGGGGGAGAGCCCTTGTAGAATAGGTAGTACACGTTTGGTTAGGGGGGCCTGCATATGGCCCCCTTTTTCACTTTTatat                                                                                               <  1:723416/112‑1 (MQ=255)
                                                               cgaAGATAACGCATCTGCGGCGCGAAATATGAAGGGGGAGAGCCCTTATAGACCAGGTAGTACACGTTTGGTTAGGGGGGCCTGCATATGGCCCCCTTTTTCACTTTTatat                                                                                               >  2:87996/1‑112 (MQ=255)
                                                               cgaAGATAACGCATCTGCGGCGCGAAATATGAAGGGGGAGAGCCCTTATAGACCAGGTAGTACACGTTTGGTTAGGGGGGCCTGCATATGGCCCCCTTTTTCACTTTTatat                                                                                               <  1:87996/112‑1 (MQ=255)
                                                               cgaAGATAACGCATCTGCGGCGCGAAATATGAAGGGGGAGAGCCCTTATAGACCAGGTAGTACACGTTTGGTTAGGGGGGCCTGCATATGGCCCCCTTTTTCACTTTTatat                                                                                               >  2:723416/1‑112 (MQ=255)
                                                                        cGCATCTGCGGCGCGAAATATGAAGGGGGAGAGCCCTTATAGACCAGGTAGTACACGTTTGATTAGGGGGGCCTGCATATGGCCCCCTTTTTCACTTTTATATCTGTGCGGTTTAATGCCGGGCAGATCACATCTCCGa                                                           >  1:381482/1‑139 (MQ=255)
                                                                                 ggcgCGAAATATGAAGGGGGAGAGCCCTTATAGACCAGGTAGTACACGTTTGGTTAGGGGGGCCTGCATATGGCCCCCTTTTTCACTTTTATATCTGTGCGGTTTAATGCCGGGCAGATCACATCTCCGAGGATTTTAg                                                  <  2:748892/139‑1 (MQ=255)
                                                                                                  gggAGAGCCCTTATAGACCAGGTAGTACACGTTTGGTTAGGGGGGCCTGCATATGGCCCCCTTTTTCACTTTTATATCTGTGCGGtt                                                                                     <  2:46786/87‑1 (MQ=255)
                                                                                                  gggAGAGCCCTTATAGACCAGGTAGTACACGTTTGGTTAGGGGGGCCTGCATATGGCCCCCTTTTTCACTTTTATATCTGTGCGGtt                                                                                     >  1:46786/1‑87 (MQ=255)
                                                                                                                      ggTAGTACACGTTTGGTTAGGGGGGCCTGCATATGGCCCCCTTTTTc                                                                                                         >  2:371957/1‑47 (MQ=255)
                                                                                                                      ggTAGTACACGTTTGGTTAGGGGGGCCTGCATATGGCCCCCTTTTTc                                                                                                         <  1:371957/47‑1 (MQ=255)
                                                                                                                       gtagtaCACGTTTGGTTAGGGGGGCCTGCATATGGCCCCCTTTTTCACTTTGATATCTGTGCGGTTTAATGCCGGGCAGATCACATCTCCGAGGATTTTAGAATGGCTGAAATTACCGCATCCCTGGTAAAAGAGCTGc            >  2:482682/1‑139 (MQ=255)
                                                                                                                            acacGTTTGGTTAGGGGGGCCTGCATATGGCCCCCTTTTTCACTTTTATATCTGTGCGGTTTAATGCCGGGCAGATCACATCTCCGAGGATTTTAGa                                                 <  2:374127/97‑1 (MQ=255)
                                                                                                                            acacGTTTGGTTAGGGGGGCCTGCATATGGCCCCCTTTTTCACTTTTATATCTGTGCGGTTTAATGCCGGGCAGATCACATCTCCGAGGATTTTAGa                                                 >  1:374127/1‑97 (MQ=255)
                                                                                                                             cacGTTTGGTTAGGGGGGCCTGCATATGGCCCCCTTTTTCACTTTTATATCTGTGCGGTTTAATGCCGGGCAGATCACATCTCCGAGGATTTTAGAATGGCTGaaa                                       >  1:175024/1‑106 (MQ=255)
                                                                                                                             cacGTTTGGTTAGGGGGGCCTGCATATGGCCCCCTTTTTCACTTTTATATCTGTGCGGTTTAATGCCGGGCAGATCACATCTCCGAGGATTTTAGAATGGCTGaaa                                       <  2:175024/106‑1 (MQ=255)
                                                                                                                                gTTTGGTTAGGGGGGCCTGCATATGGCCCCCTTTTTCACTTTTATATCTGTGCGGTTTAATGCCGGGCAGATCACATCTCCGAGGATTTTAGAATGGCTGAAATTACCGCATCCCTGGTAAAAGAGCTGCGTGAGCGTa   <  1:229033/139‑1 (MQ=255)
                                                                                                                                 tttGGTTAGGGGGGCCTGCATATGGCCCCCTTTTTCACTTTTATATCTGTGCGGTTTAATGCCGGGCAGATCACATCTCCGAGGATTTTAGAATGGCTGAAATTACCGCATCCCTGGTAAAAGAGCTGCGTGAGCGTaa  >  1:116823/1‑138 (MQ=255)
                                                                                                                                 tttGGTTAGGGGGGCCTGCATATGGCCCCCTTTTTCACTTTTATATCTGTGCGGTTTAATGCCGGGCAGATCACATCTCCGAGGATTTTAGAATGGCTGAAATTACCGCATCCCTGGTAAAAGAGCTGCGTGAGCGTAc  <  1:482682/139‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                  
AGAAAGGCGGCAAGCTCGAGAATTCCCGGGAGCTTACATCAGTAAGTGACCGGGATGAGCGAGCGAAGATAACGCATCTGCGGCGCGAAATATGAAGGGGGAGAGCCCTTATAGACCAGGTAGTACACGTTTGGTTAGGGGGCCTGCATATGGCCCCCTTTTTCACTTTTATATCTGTGCGGTTTAATGCCGGGCAGATCACATCTCCGAGGATTTTAGAATGGCTGAAATTACCGCATCCCTGGTAAAAGAGCTGCGTGAGCGTAC  >  NC_000913/190637‑190903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: