Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,488,579	(C)7→8	coding (1485/1503 nt)	yjgR ←	DUF853 family protein with NTPase fold

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,488,572	1	.	C	100.0%	137.8 / NA	37	R498G (AGA→GGA)	yjgR	DUF853 family protein with NTPase fold
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (21/16);  total (21/16)

AGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCT‑CCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCCGCCGCGTGGTC  >  NC_000913/4488446‑4488682                                                                                                                                |                                                                                                               aGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCT‑ccccccccagca                                                                                                    >  1:169654/1‑134 (MQ=255) aGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCT‑ccccccccagca                                                                                                    >  1:635651/1‑134 (MQ=255) aGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCT‑ccccccccagca                                                                                                    >  1:30898/1‑134 (MQ=255) aGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCT‑cc                                                                                                              <  1:657207/129‑1 (MQ=255) aGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCT‑cc                                                                                                              >  2:657207/1‑129 (MQ=255)   cAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTCCCCCCCCAGCaaa                                                                                                   <  2:379335/139‑1 (MQ=255)              tCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGGCTTACCTTCTTCTCCCCCCCCAGCAAACTCCCCAACAt                                                                                        <  2:169654/139‑1 (MQ=255)                        cTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTCCCCCCCCAGCAAACTCCCCAACATCCCGCGTACa                                                                              >  1:431421/1‑139 (MQ=255)                           aaaaaTAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTCCCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATc                                                                           >  2:533061/1‑139 (MQ=255)                                taatTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTCCCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGAtt                                                                      >  2:639695/1‑139 (MQ=255)                                                   tGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTCCCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAgcggcg                                                   >  1:245989/1‑139 (MQ=255)                                                        tggggtgggCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTCCCCCCCCAGCAAACTCCCCAAc                                                                                          <  2:477096/95‑1 (MQ=255)                                                        tggggtgggCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTCCCCCCCCAGCAAACTCCCCAAc                                                                                          >  1:477096/1‑95 (MQ=255)                                                        tggggtgggCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTCCCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCtttt                                              >  2:181032/1‑139 (MQ=255)                                                          gggtgggCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTCCCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTgg                                            >  1:451102/1‑139 (MQ=255)                                                            gtgggCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTCCCCCCCCAGCaaa                                                                                                   <  1:263101/82‑1 (MQ=255)                                                            gtgggCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTCCCCCCCCAGCaaa                                                                                                   >  2:263101/1‑82 (MQ=255)                                                            gtgggCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTCCCCCCCCAGCAAACTCCCCAACATcc                                                                                      >  2:358465/1‑95 (MQ=255)                                                            gtgggCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTCCCCCCCCAGCAAACTCCCCAACATcc                                                                                      <  1:358465/95‑1 (MQ=255)                                                             tgggCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTCCCCCCCCAGCAAACTCCCCAACATcc                                                                                      >  1:715173/1‑94 (MQ=255)                                                             tgggCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTCCCCCCCCAGCAAACTCCCCAACATcc                                                                                      <  2:715173/94‑1 (MQ=255)                                                               ggCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTCCCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCttt                                               >  1:699551/1‑131 (MQ=255)                                                               ggCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTCCCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCttt                                               <  2:699551/131‑1 (MQ=255)                                                                     aTAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTCCCCCCCCAGCAAACTCCCCAAc                                                                                          >  2:284961/1‑82 (MQ=255)                                                                     aTAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTCCCCCCCCAGCAAACTCCCCAAc                                                                                          <  1:284961/82‑1 (MQ=255)                                                                     aTAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTCCCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGc                                 >  1:198090/1‑139 (MQ=255)                                                                      tAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTCCCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGca                                <  1:533061/139‑1 (MQ=255)                                                                         gACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTCCCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGcacca                             >  2:598310/1‑139 (MQ=255)                                                                             cgcCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTCCCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGcaccacacc                         >  1:728215/1‑139 (MQ=255)                                                                              gcCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTCCCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGcaccacacca                        >  2:331996/1‑139 (MQ=255)                                                                              gcCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTCCCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGcaccacacca                        >  1:394110/1‑139 (MQ=255)                                                                               ccAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTCCCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCAt                       >  1:259023/1‑139 (MQ=255)                                                                                               cAAAGACAAATCTCGCCTGACTTACCTTCTTCTCCCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCCGCcgcg       <  1:598310/139‑1 (MQ=255)                                                                                               cAAAGACAAATCTCGCCTGACTTACCTTCTTCTCCCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCCGCcgcg       <  2:394110/139‑1 (MQ=255)                                                                                               cAAAGACAAATCTCGCCTGACTTACCTTCTTCTCCCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCCGCcgcg       <  2:728215/139‑1 (MQ=255)                                                                                                   gACAAATCTCGCCTGACTTACCTTCTTCTCCCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCCGCCGCGTGGt   >  1:327922/1‑139 (MQ=255)                                                                                                    aCAAATCTCGCCTGACTTACCTTCTTCTCCCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCCGCCGCGTGGTc  <  1:181032/139‑1 (MQ=255)                                                                                                    aCAAATCTCGCCTGACTTACCTTCTTCTCCCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCCGCCGCGTGGTc  <  2:259023/139‑1 (MQ=255)                                                                                                        aTCTCGCCTGACTTACCTTCTTCTCCCCCCCCAGCAAACTCCCCAAc                                                                                          >  1:78315/1‑47 (MQ=255)                                                                                                        aTCTCGCCTGACTTACCTTCTTCTCCCCCCCCAGCAAACTCCCCAAc                                                                                          <  2:78315/47‑1 (MQ=255)                                                                                                        aTCTCGCCTGACTTACCTTCTTCTCCCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCCGCcgcg       <  2:362862/130‑1 (MQ=255)                                                                                                        aTCTCGCCTGACTTACCTTCTTCTCCCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCCGCcgcg       >  1:362862/1‑130 (MQ=255)                                                                                                                                |                                                                                                               AGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCT‑CCCCCCCAGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCCGCCGCGTGGTC  >  NC_000913/4488446‑4488682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑