| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,002,217 | 1 | . | G | 25.0% | 44.3 / 23.2 | 24 | coding (411/1071 nt) | elfG | putative fimbrial‑like adhesin protein |
| Reads supporting (aligned to +/- strand): ref base . (10/8); new base G (4/2); total (14/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCATTCCTCCTA > NC_000913/1002081‑1002350 | gggCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑gg < 1:539367/139‑1 (MQ=255) ggATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGGATACCCGa < 2:586430/139‑1 (MQ=255) ttAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGGATACCCGATGGCATAttt > 2:355658/1‑139 (MQ=255) tAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGGATACCCGATGGCATATTTg < 1:355658/139‑1 (MQ=255) gCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCg > 2:724427/1‑139 (MQ=255) gCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGGATACCCGATGGCATATTTGCGACCCTGGCgg > 2:86067/1‑139 (MQ=255) cGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGGATACCCGATg < 1:312650/117‑1 (MQ=255) cGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGGATACCCGATg > 2:312650/1‑117 (MQ=255) cGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGt < 1:86067/139‑1 (MQ=255) gTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGttt > 2:395858/1‑139 (MQ=255) tATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCtt > 2:48281/1‑139 (MQ=255) ttGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCttt > 2:370589/1‑139 (MQ=255) ggTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTg > 2:712713/1‑139 (MQ=255) gTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGt < 2:751463/77‑1 (MQ=255) gTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGt > 1:751463/1‑77 (MQ=255) gCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTg > 2:102069/1‑139 (MQ=255) gCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTg > 1:159165/1‑139 (MQ=255) cGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCa < 2:143113/90‑1 (MQ=255) cGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCa > 1:143113/1‑90 (MQ=255) gTTCCTTTCGAAAACGTGGGCACA‑GGGGGGGATAcc > 1:719141/1‑36 (MQ=255) gTTCCTTTCGAAAACGTGGGCACA‑GGGGGGGATAcc < 2:719141/36‑1 (MQ=255) cTTTCGAAAACGTGGGCACA‑GGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGgt < 1:754155/139‑1 (MQ=255) ttCGAAAACGTGGGCACA‑GGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGgtcg < 1:48281/139‑1 (MQ=255) cGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGgtcgt < 1:724427/139‑1 (MQ=255) ggCACA‑GGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCATTCCTCCTa > 1:239891/1‑139 (MQ=255) | GGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑GGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTCGGTCGTCATTCCTCCTA > NC_000913/1002081‑1002350 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |