Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	783,866	C→T	S234L (TCA→TTA)	pnuC →	nicotinamide riboside transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	783,866	0	C	T	100.0%	180.4 / NA	52	S234L (TCA→TTA)	pnuC	nicotinamide riboside transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/29);  total (23/29)

ATTATTAACGTGATTAGCGTCGTTATTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTCACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGCCCATGACAAGGTTGATATTCCATCTGAATGGTGGCGTGCTCAATCTGATAGTGATCCATCAGGT  >  NC_000913/783733‑784000                                                                                                                                      |                                                                                                                                       attattAACGTGATTAGCGTCGTTATTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTTAcgcg                                                                                                                                   <  2:414216/139‑1 (MQ=255)             aTTAGCGTCGTTATTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTTACGCGCGCTGTCCCAtt                                                                                                                       >  1:334562/1‑139 (MQ=255)             aTTAGCGTCGTTATTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTTACGCGCGCTGTCCCAtt                                                                                                                       >  1:635705/1‑139 (MQ=255)                     cgtTATTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACATTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTTAcgcgcg                                                                                                                                 <  2:363551/121‑1 (MQ=255)                     cgtTATTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACATTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTTAcgcgcg                                                                                                                                 >  1:363551/1‑121 (MQ=255)                              tGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTTACGCGCGCTGTCCCATTAATGGTGATGATGTGaa                                                                                                      <  1:687780/139‑1 (MQ=255)                                        ggCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTTACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGaca                                                                                            <  2:392281/139‑1 (MQ=255)                                                       tctcTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTTACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGt                                                                             >  1:674408/1‑139 (MQ=255)                                                        ctctGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTTACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTg                                                                            <  2:392248/139‑1 (MQ=255)                                                              aGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTTACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTc                                                                      <  1:276778/139‑1 (MQ=255)                                                              aGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTTACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTc                                                                      <  1:277291/139‑1 (MQ=255)                                                                tACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTTACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTg                                                                    >  2:292406/1‑139 (MQ=255)                                                                 aCATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTTACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTgg                                                                   >  1:619729/1‑139 (MQ=255)                                                                  catcatCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTTACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGc                                                                  >  1:158802/1‑139 (MQ=255)                                                                       tcCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTTACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGcc                                                                 <  1:409978/135‑1 (MQ=255)                                                                       tcCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTTACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGcc                                                                 >  2:409978/1‑135 (MQ=255)                                                                       tcCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTTACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGCCCATg                                                             >  2:114122/1‑139 (MQ=255)                                                                        ccTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTTACGCGCGCTGt                                                                                                                             <  2:417825/74‑1 (MQ=255)                                                                        ccTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTTACGCGCGCTGt                                                                                                                             >  1:417825/1‑74 (MQ=255)                                                                        ccTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTTACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGCCCATGa                                                            >  1:190191/1‑139 (MQ=255)                                                                                 tATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTTACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGt                                                                       <  1:643242/119‑1 (MQ=255)                                                                                 tATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTTACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGt                                                                       >  2:643242/1‑119 (MQ=255)                                                                                    tGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTTACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGCCCATGACAAGGTTGatat                                                <  1:500067/139‑1 (MQ=255)                                                                                    tGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTTACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGCCCATGACAAGGTTGatat                                                <  2:588956/139‑1 (MQ=255)                                                                                      cgcTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTTACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGt                                                                       <  1:565033/114‑1 (MQ=255)                                                                                      cgcTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTTACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGt                                                                       >  2:565033/1‑114 (MQ=255)                                                                                      cgcTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTTACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGCCCATGACAAGGTTGATATTc                                              >  2:467622/1‑139 (MQ=255)                                                                                      cgcTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTTACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGCCCATGACAAGGTTGATATTc                                              >  2:405714/1‑139 (MQ=255)                                                                                      cgcTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTTACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGCCCATGACAAGGTTGATATTc                                              <  1:77987/139‑1 (MQ=255)                                                                                         tCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTTACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGCCCATGACAAGGTTGATATTCCAt                                           <  2:635705/139‑1 (MQ=255)                                                                                             cGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTTACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGCCCATGACAAGGTTGATATTCCATCTGa                                       >  1:3766/1‑139 (MQ=255)                                                                                                cAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTTACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGCCCATGACAAGGTTGATATTCCATCTGAAtg                                    >  2:760332/1‑139 (MQ=255)                                                                                                     ggATGTGGATCAACAGCGCACGTGAAAGAGGCTTACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGCCCATGACAAGGTTGATATTCCATCTGAATGGTGGc                               <  1:114122/139‑1 (MQ=255)                                                                                                        tgtgGATCAACAGCGCACGTGAAAGAGGCTTACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGCCCATGACAAGGTTGATATTCCATCTGAATGGTGGCGTg                            <  2:619729/139‑1 (MQ=255)                                                                                                          tgGATCAACAGCGCACGTGAAAGAGGCTTACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGCCCATGACAAGGTTGATATTCCATCTGAATGGTGGCGTGCt                          <  1:292406/139‑1 (MQ=255)                                                                                                          tgGATCAACAGCGCACGTGAAAGAGGCTTACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGCCCATGACAAGGTTGATATTCCATCTGAATGGTGGCGTGCt                          <  1:746344/139‑1 (MQ=255)                                                                                                              tCAACAGCGCACGTGAAAGAGGCTTACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGCCCATGACAAGGTTGATATTCCATCTGAATGGTGGCGTGCTCAAt                      <  2:334562/139‑1 (MQ=255)                                                                                                                    gcgcACGTGAAAGAGGCTTACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGCCCATGACAAGGTTGATATTCCATCTGAATGGTGGCGTGCTCAATCTGATa                >  2:261239/1‑139 (MQ=255)                                                                                                                    gcgcACGTGAAAGAGGCTTACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGCCCATGACAAGGTTGATATTCCATCTGAATGGTGGCGTGCTCAATCTGATa                >  1:405347/1‑139 (MQ=255)                                                                                                                     cgcACGTGAAAGAGGCTTACGCGCGCTGTCCCATTAATGGTGATGATCTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGCCCATGACAAGGTTGATATTCCATCTGAATGGTGGCGTGCTCAATCTGATAg               <  1:467622/139‑1 (MQ=255)                                                                                                                       cACGTGAAAGAGGCTTACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCa                                                                                                <  2:50528/56‑1 (MQ=255)                                                                                                                       cACGTGAAAGAGGCTTACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCa                                                                                                >  1:50528/1‑56 (MQ=255)                                                                                                                       cACGTGAAAGAGGCTTACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGCCCATGAc                                                           <  2:92913/93‑1 (MQ=255)                                                                                                                       cACGTGAAAGAGGCTTACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGCCCATGAc                                                           <  2:384592/93‑1 (MQ=255)                                                                                                                       cACGTGAAAGAGGCTTACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGCCCATGAc                                                           >  1:92913/1‑93 (MQ=255)                                                                                                                       cACGTGAAAGAGGCTTACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGCCCATGAc                                                           >  1:384592/1‑93 (MQ=255)                                                                                                                            gAAAGAGGCTTACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGCCCATGACAAGGTTGATATTCCATCTGAATGGTGGCGTGCTCAATCTGATAGTg             <  2:256437/134‑1 (MQ=255)                                                                                                                            gAAAGAGGCTTACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGCCCATGACAAGGTTGATATTCCATCTGAATGGTGGCGTGCTCAATCTGATAGTg             >  1:256437/1‑134 (MQ=255)                                                                                                                             aaaGAGGCTTACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGCCCATGACCAGGTTGATATTCCATCTGAATGGTGGCGTGCTCAATCTGATAGTGATCCAt       <  2:674408/139‑1 (MQ=255)                                                                                                                             aaaGAGGCTTACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGCCCATGACAAGGTTGATATTCCATCTGAATGGTGGCGTGCTCAATCTGATAGTGATCCAt       <  2:158802/139‑1 (MQ=255)                                                                                                                                 agGCTTACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGCCCATGACAAGGTTGATATTCCATCTGAATGGTGGCGTGCTCAATCTGATAGTGATCCATCAgg   <  1:760332/139‑1 (MQ=255)                                                                                                                                  ggCTTACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGCCCATGACAAGGTTGATATTCCATCTGAATGGTGGCGTGCTCAATCTGATAGTGATCCATCAGGt  <  2:190191/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       ATTATTAACGTGATTAGCGTCGTTATTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCCTGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTCACGCGCGCTGTCCCATTAATGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGCCCATGACAAGGTTGATATTCCATCTGAATGGTGGCGTGCTCAATCTGATAGTGATCCATCAGGT  >  NC_000913/783733‑784000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑