Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 791,349 A→G V236A (GTA→GCA)  galE ← UDP‑galactose‑4‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913791,3490AG100.0% 135.6 / NA 38V236A (GTA→GCA) galEUDP‑galactose‑4‑epimerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/19);  total (19/19)

GCCGCAGGCTTTGCTGAAGGCATTAACCACGTCCAGCACGCTGTTGCCTACGCCAGCGCCGAGGTTGTAGATGTGTACGCCTGGCTTGTTCGCCAGTTTTTCCATCGCCACGACGTGACCGTCCGCCAGATCCATTACGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATGTATGGCATCAGGTTATTCGGAATGCCTTGCG  >  NC_000913/791213‑791478
                                                                                                                                        |                                                                                                                                 
gCCGCAGGCTTTGCTGAAGGCATTAACCACGTCCAGCACGCTGTTGCCTACGCCAGCGCCGAGGTTGTAGATGTGTACGCCTGGCTTGTTCGCCAGTTTTTCCATCGCCACGACGTGACCGTCCGCCAGATCCATTGCg                                                                                                                                 <  2:395420/139‑1 (MQ=255)
                aaGGCATTAACCACGTCCAGCACGCTGTTGCCTACGCCAGCGCCGAGGTTGTAGATGTGTACGCCTGGCTTGTTCGCCAGTTTTTCCATCGCCACGACGTGACCGTCCGCCAGATCCATTGCGTGGATGTAATCGCGTa                                                                                                                 <  2:465429/139‑1 (MQ=255)
                 aGGCATTAACCACGTCCAGCACGCTGTTGCCTACGCCAGCGCCGAGGTTGTAGATGTGTACGCCTGGCTTGTTCGCCAGTTTTTCCATCGCCACGACGTGACCGTCCGCCAGATCCATTGCGTGGATGTAATCGCGTAc                                                                                                                >  1:230854/1‑139 (MQ=255)
                   gCATTAACCACGTCCAGCACGCTGTTGCCTACGCCAGCGCCGAGGTTGTAGATGTGTACGCCTGGCTTGTTCGCCAGTTTTTCCATCGCCACGACGTGACCGTCCGCCAGATCCATTGCGTGGATGTAATCGCGTACGc                                                                                                              <  2:230854/139‑1 (MQ=255)
                       tAACCACGTCCAGCACGCTGTTGCCTACGCCAGCGCCGAGGTTGTAGATGTGTACGCCTGGCTTGTTCGCCAGTTTTTCCATCGCCACGACGTGACCGTCCGCCAGATCCATTGCGTGGATGTAATCGCGTACGCCAGt                                                                                                          >  1:40323/1‑139 (MQ=255)
                                   gCACGCTGTTGCCTACGCCAGCGCCGAGGTTGTAGATGTGTACGCCTGGCTTGTTCGCCAGTTTTTCCATCGCCACGACGTGACCGTCCGCCAGATCCATTGCGTGGATGTAATCGCGTACGCCAGTACCATCTtcggt                                                                                              >  1:536897/1‑139 (MQ=255)
                                    cACGCTGTTGCCTACGCCAGCGCCGAGGTTGTAGATGTGTACGCCTGGCTTGTTCGCCAGTTTTTCCATCGCCACGACGTGACCGTCCGCCAGATCCATTGCGTgg                                                                                                                              <  1:336411/106‑1 (MQ=255)
                                    cACGCTGTTGCCTACGCCAGCGCCGAGGTTGTAGATGTGTACGCCTGGCTTGTTCGCCAGTTTTTCCATCGCCACGACGTGACCGTCCGCCAGATCCATTGCGTgg                                                                                                                              >  2:336411/1‑106 (MQ=255)
                                    cACGCTGTTGCCTACGCCAGCGCCGAGGTTGTAGATGTGTACGCCTGGCTTGTTCGCCAGTTTTTCCATCGCCACGACGTGACCGTCCGCCAGATCCATTGCGTGGATGTAATCGCGTACGCCAGTACCATCTtcggtc                                                                                             >  1:254388/1‑139 (MQ=255)
                                         tGTTGCCTACGCCAGCGCCGAGGTTGTAGATGTGTACGCCTGGCTTGTTCGCCAGTTTTTCCATCGCCACGACGTGACCGTCCGCCAGATCCATTGCGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATa                                                                                        >  1:661797/1‑139 (MQ=255)
                                             gCCTACGCCAGCGCCGAGGTTGTAGATGTGTACGCCTGGCTTGTTCGCCAGTTTTTCCATCGCCACGACGTGACCGTCCGCCAGATCCATTGCGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCg                                                                                    >  1:535936/1‑139 (MQ=255)
                                             gCCTACGCCAGCGCCGAGGTTGTAGATGTGTACGCCTGGCTTGTTCGCCAGTTTTTCCATCGCCACGACGTGACCGTCCGCCAGATCCATTGCGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCg                                                                                    >  2:154516/1‑139 (MQ=255)
                                                                  gTAGATGTGTACGCCTGGCTTGTTCGCCAGTTTTTCCATCGCCACGACGTGACCGTCCGCCAGATCCATTGCGTGGATGTAATCGCGTACGCCAGTACCATCTtcgg                                                                                               >  1:499583/1‑107 (MQ=255)
                                                                  gTAGATGTGTACGCCTGGCTTGTTCGCCAGTTTTTCCATCGCCACGACGTGACCGTCCGCCAGATCCATTGCGTGGATGTAATCGCGTACGCCAGTACCATCTtcgg                                                                                               <  2:499583/107‑1 (MQ=255)
                                                                  gTAGATGTGTACGCCTGGCTTGTTCGCCAGTTTTTCCATCGCCACGACGTGACCGTCCGCCAGATCCATTGCGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAg                                                               >  2:266582/1‑139 (MQ=255)
                                                                          gtACGCCTGGCTTGTTCGCCAGTTTTTCCATCGCCACGACGTGACCGTCCGCCAGATCCATTGCGt                                                                                                                                <  1:631601/66‑1 (MQ=255)
                                                                          gtACGCCTGGCTTGTTCGCCAGTTTTTCCATCGCCACGACGTGACCGTCCGCCAGATCCATTGCGt                                                                                                                                >  2:631601/1‑66 (MQ=255)
                                                                          gtACGCCTGGCTTGTTCGCCAGTTTTTCCATCGCCACGACGTGACCGTCCGCCAGATCCATTGCGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACg                                                       >  1:81589/1‑139 (MQ=255)
                                                                           tACGCCTGGCTTGTTCGCCAGTTTTTCCATCGCCACGACGTGACCGTCCGCCAGATCCATTGCGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGa                                                                                          >  2:23632/1‑103 (MQ=255)
                                                                           tACGCCTGGCTTGTTCGCCAGTTTTTCCATCGCCACGACGTGACCGTCCGCCAGATCCATTGCGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGa                                                                                          <  1:23632/103‑1 (MQ=255)
                                                                           tACGCCTGGCTTGTTCGCCAGTTTTTCCATCGCCACGACGTGACCGTCCGCCAGATCCATTGCGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACgg                                                      >  1:577932/1‑139 (MQ=255)
                                                                                cTGGCTTGTTCGCCAGTTTTTCCATCGCCACGACGTGACCGTCCGCCAGATCCATTGCGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCaaa                                                                            >  1:292433/1‑112 (MQ=255)
                                                                                cTGGCTTGTTCGCCAGTTTTTCCATCGCCACGACGTGACCGTCCGCCAGATCCATTGCGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCaaa                                                                            <  2:292433/112‑1 (MQ=255)
                                                                                     ttGTTCGCCAGTTTTTCTATCGCCACGACGTGACCGTCCGCCAGATCCATTGCGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCaaa                                                                            <  2:471170/107‑1 (MQ=255)
                                                                                     ttGTTCGCCAGTTTTTCCATCGCCACGACGTGACCGTCCGCCAGATCCATTGCGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCaaa                                                                            >  1:471170/1‑107 (MQ=255)
                                                                                           gCCAGTTTTTCCATCGCCACGACGTGACCGTCCGCCAGATCCATTGCGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTggg                                      <  2:536897/139‑1 (MQ=255)
                                                                                           gCCAGTTTTTCCATCGCCACGACGTGACCGTCCGCCAGATCCATTGCGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTggg                                      <  2:561210/139‑1 (MQ=255)
                                                                                           gCCAGTTTTTCCATCGCCACGACGTGACCGTCCGCCAGATCCATTGCGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTggg                                      <  2:40323/139‑1 (MQ=255)
                                                                                             cAGTTTTTCCATCGCCACGACGTGACCGTCCGCCAGATCCATTGCGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCg                                    <  2:254388/139‑1 (MQ=255)
                                                                                             cAGTTTTTCCATCGCCACGACGTGACCGTCCGCCAGATCCATTGCGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCg                                    >  2:366109/1‑139 (MQ=255)
                                                                                             cAGTTTTTCCATCGCCACGACGTGACCGTCCGCCAGATCCATTGCGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCg                                    <  1:366109/139‑1 (MQ=255)
                                                                                                          gCCACGACGTGACCGTCCGCCAGATCTATTGCGTGGATGTAATcgcg                                                                                                                   >  2:463479/1‑47 (MQ=255)
                                                                                                          gCCACGACGTGACCGTCCGCCAGATCTATTGCGTGGATGTAATcgcg                                                                                                                   <  1:463479/47‑1 (MQ=255)
                                                                                                          gCCACGACGTGACCGTCCGCCAGATCCATTGCGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATGTATGGCATCa                       >  1:435195/1‑139 (MQ=255)
                                                                                                           ccACGACGTGACCGTCCGCCAGATCCATTGCGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATGTATGGCATCAg                      <  2:81589/139‑1 (MQ=255)
                                                                                                            cACGACGTGACCGTCCGCCAGATCCATTGCGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATGTATGGCATCAgg                     <  2:577932/139‑1 (MQ=255)
                                                                                                                      ccGTCCGCCAGATCCATTGCGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATGTATGGCATCAGGTTATTCGGaa           <  1:154516/139‑1 (MQ=255)
                                                                                                                               aGATCCATTGCGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATGTATGGCATCAGGCTATTCGGAATGCCTTGCg  <  2:435195/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                 
GCCGCAGGCTTTGCTGAAGGCATTAACCACGTCCAGCACGCTGTTGCCTACGCCAGCGCCGAGGTTGTAGATGTGTACGCCTGGCTTGTTCGCCAGTTTTTCCATCGCCACGACGTGACCGTCCGCCAGATCCATTACGTGGATGTAATCGCGTACGCCAGTACCATCTTCGGTCGGATAATCGTTACCAAAAATCGCCAGCGAGTCGCGACGGCCTACAGCAACCTGGGCGATGTATGGCATCAGGTTATTCGGAATGCCTTGCG  >  NC_000913/791213‑791478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑