Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,121,934	A→G	I225T (ATC→ACC)	pyrC ←	dihydro‑orotase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,121,934	0	A	G	100.0%	128.1 / NA	35	I225T (ATC→ACC)	pyrC	dihydro‑orotase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/17);  total (18/17)

AAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTG  >  NC_000913/1121799‑1122068                                                                                                                                        |                                                                                                                                       aaGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTgg                                                                                                                                     <  1:702425/139‑1 (MQ=255)           cgcAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATa                                                                                                                           <  1:347884/139‑1 (MQ=255)                              tttGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCt                                                                                                        <  1:682110/139‑1 (MQ=255)                                tGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTcc                                                                                                      >  2:129983/1‑139 (MQ=255)                                  cGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCaa                                                                                                    <  2:778865/139‑1 (MQ=255)                                        cGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTa                                                                                                                                  <  2:220325/103‑1 (MQ=255)                                        cGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTa                                                                                                                                  >  1:220325/1‑103 (MQ=255)                                          tGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGCGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCata                                                                                            >  1:466882/1‑139 (MQ=255)                                               gtgGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGtt                                                                                       >  1:498299/1‑139 (MQ=255)                                                tgGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTg                                                                                      <  2:498299/139‑1 (MQ=255)                                                 ggCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGc                                                                                     >  1:188814/1‑139 (MQ=255)                                                              tACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAg                                                                        >  1:78443/1‑139 (MQ=255)                                                                  gAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGacgca                                                                                                             >  2:101921/1‑98 (MQ=255)                                                                  gAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGacgca                                                                                                             <  1:101921/98‑1 (MQ=255)                                                                  gAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGAtgc                                                                    <  1:249309/139‑1 (MQ=255)                                                                   aGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCaa                                                                                                    <  2:126870/106‑1 (MQ=255)                                                                   aGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCaa                                                                                                    >  1:126870/1‑106 (MQ=255)                                                                           tCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTg                                                           >  1:536019/1‑139 (MQ=255)                                                                             gATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAAc                                                                                                   <  1:52743/97‑1 (MQ=255)                                                                             gATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAAc                                                                                                   >  2:52743/1‑97 (MQ=255)                                                                                       gCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGacgca                                                                                                             >  2:542417/1‑77 (MQ=255)                                                                                       gCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGacgca                                                                                                             <  1:542417/77‑1 (MQ=255)                                                                                                             gCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGTACGCACGCCTCCAAc                                                                                                   <  2:521946/65‑1 (MQ=255)                                                                                                             gCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCt                                                                                                        <  2:107004/60‑1 (MQ=255)                                                                                                             gCCTGTTGGTGAATATTACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCt                                                                                                        >  1:107004/1‑60 (MQ=255)                                                                                                             gCCTGTTGGTGAATATTACATTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAAc                                                                                                   >  1:521946/1‑65 (MQ=255)                                                                                                                            ttACGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCAt          >  1:306571/1‑139 (MQ=255)                                                                                                                               cGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCa                                                                                              <  2:339496/52‑1 (MQ=255)                                                                                                                               cGTTTGAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCa                                                                                              >  1:339496/1‑52 (MQ=255)                                                                                                                                    gAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTcc                                                                                                      <  2:134264/39‑1 (MQ=255)                                                                                                                                    gAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTcc                                                                                                      >  1:134264/1‑39 (MQ=255)                                                                                                                                    gAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCAttt                                   >  2:288138/1‑106 (MQ=255)                                                                                                                                    gAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCAttt                                   <  1:288138/106‑1 (MQ=255)                                                                                                                                    gAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTtggtg  <  2:536019/139‑1 (MQ=255)                                                                                                                                    gAGGGTGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTtggtg  >  2:683587/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                       AAGCAGCCCGCGCAGCCGCAACTGCTCTCTTTGCGATGACGTGCATGTGGCGCAGAATCCGTACCGAGGAATACTCGATTAAAACCGCTGGCGACCAGTTCACGCAATGCCTGTTGGTGAATATTACGTTTGAGGATGGGTAGACAATACAGGTGCGGACGCACGCCTCCAACCAGCATATGGTTGCGGTTAAACATCAGATGCTGCGGAGTGATGGTGGCAGCCAGCCGTTCATTTCCGTCACGGACATAGTCGGCAGCATCTTTGGTG  >  NC_000913/1121799‑1122068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑