Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,225,697 T→C I128M (ATA→ATG minC ← inhibitor of FtsZ ring polymerization

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,225,6970TC100.0% 71.0 / NA 20I128M (ATA→ATGminCinhibitor of FtsZ ring polymerization
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/10);  total (10/10)

CGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAACGCACCGGGGTATCTATTAAACGCGTTTTTGTGACCGGCGTTGTATTTTGCGCTGGAGCCTGCGGTGTGGGAGCTGGACGTGGCGCTTTTTCCTTTCCTTCCGTCAGGATAGGCAGCCCCATCTTTTCAATTTCGGCTTTAAGTTG  >  NC_000913/1225562‑1225828
                                                                                                                                       |                                                                                                                                   
cgcACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAACGCACCGGGGTATCCAtt                                                                                                                                  >  2:658434/1‑139 (MQ=255)
     gACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAACGCACCGGGGTATCCATTAAAcg                                                                                                                             >  2:452006/1‑139 (MQ=255)
                atGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAACGCACCGGGGTATCCATTAAACGCGTTTTTGTGa                                                                                                                  <  1:452006/139‑1 (MQ=255)
                   ccATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAACGCACCGGGGTATCCATTAAACGCGTTTTTGTGACCg                                                                                                               <  1:444714/139‑1 (MQ=255)
                                  ttCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAACGCACCGGGGTATCCATTAAACGCGTTTTTGTGACCGGCGTTGTATTTTgcg                                                                                                >  2:221562/1‑139 (MQ=255)
                                   tCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAACGCACCGGGGTATCCATTAAACGCGTTTTTGTGACCGGCGTTGTATTTTgcgc                                                                                               >  1:251041/1‑139 (MQ=255)
                                    cccATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAACGCACCGGGGTATCCATTAAACGCGTTTTTGTGACCGGCGTTGTATTTTGCGCt                                                                                              <  2:251041/139‑1 (MQ=255)
                                            caatcaatTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAACGCACCGGGGTATCCATTAAACGCGTTTTTGTGACCGGCGTTGTATTTTGCGCTGGAGCCTg                                                                                      >  1:181354/1‑139 (MQ=255)
                                            caatcaatTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAACGCACCGGGGTATCCATTAAACGCGTTTTTGTGACCGGCGTTGTATTTTGCGCTGGAGCCTg                                                                                      <  2:625626/139‑1 (MQ=255)
                                             aatcaatTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAACGCACCGGGGTATCCATTAAACGCGTTTTTGTGACCGGCGTTGTATTTTGCGCTGGAGCCTGc                                                                                     <  2:181354/139‑1 (MQ=255)
                                                                        gCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAACGCACCGGGGTATCCATTAAACGCGTTTTTGTGACCGGCGTTGTATTTTGCGCTGGAGCCTGCGGTGTGGGAGCTGGACGTGGCGCtttt                                                          >  1:738238/1‑139 (MQ=255)
                                                                                 aaTCAGATCACATTGTGGAGCATAAATACGCTGACCGGAACGCACCGGGGTATCCATTAAACGCGTTTTTGTGACCGGCGTTGTATTTTGCGCTGGAGCCTGCGGTGTGGGAGCTGGACGTGGCGCTTTttcctttcct                                                 <  1:62597/139‑1 (MQ=255)
                                                                                            aTTGTGGAGCATAAATACGCTGACCGGAACGCACCGGGGTATCCATTAAACGCGTTTTTGTGACCGGCGTTGTATTTTGCGCTGGAGCCTGCGGTGTGGGAGCTGGACGTGGCGCTTTTTCCTTTCCTTCCGTCAGGAt                                      >  2:129469/1‑139 (MQ=255)
                                                                                               gtgGAGCATAAATACGCTGACCGGAACGCACCGGGGTATCCATTAAACGCGTTTTTGTGACCGGCGTTGTATTTTGCGCTGGAGCCTGCGGTGTGGGAGCTGGACGTGGCGCTTTTTCCTTTCCTTCCGTCAGGATAgg                                   >  2:784228/1‑139 (MQ=255)
                                                                                                     cATAAATACGCTGACCGGAACGCACCGGGGTATCCATTAAACGCGTTTTTGTGACCGGCGTTGTATTTTGCGCTGGAGCCTGCgg                                                                                   >  2:27711/1‑85 (MQ=255)
                                                                                                     cATAAATACGCTGACCGGAACGCACCGGGGTATCCATTAAACGCGTTTTTGTGACCGGCGTTGTATTTTGCGCTGGAGCCTGCgg                                                                                   <  1:27711/85‑1 (MQ=255)
                                                                                                      aTAAATACGCTGACCGGAACGCACCGGGGTATCCATTAAACGCGTTTTTGTGACCGGCGTTGTATTTTGCGCTGGAGCCTGCGGTGTGGGAGCTGGACGTGGCGCTTTTTCCTTTCCTTCCGTCAGGATAGGCAGcccc                            <  1:129469/139‑1 (MQ=255)
                                                                                                                                gggTATCCATTAAACGCGTTTTTGTGACCGGCGTTGTATTTTGCGCTGGAGCCTGCGGTGTGGGAGCTGGACGTGGCGCTTTTTCCTTTCCTTCCg                                             <  1:640952/96‑1 (MQ=255)
                                                                                                                                gggTATCCATTAAACGCGTTTTTGTGACCGGCGTTGTATTTTGCGCTGGAGCCTGCGGTGTGGGAGCTGGACGTGGCGCTTTTTCCTTTCCTTCCg                                             >  2:640952/1‑96 (MQ=255)
                                                                                                                                gggTATCCATTAAACGCGTTTTTGTGACCGGCGTTGTATTTTGCGCTGGAGCCTGCGGTGTGGGAGCTGGACGTGGCGCTTTTTCCTTTCCTTCCGTCAGGATAGGCAGCCCCATCTTTTCAATTTCGGCTTTAAGTTg  <  2:620967/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                   
CGCACGACCGCGCATCATGCCATAGACATGAATGTTCCCATCGGCAATCAATTCGGCCCCAGCGCTAACGTGGCTTGTAACAATCAGATCACATTGTGGAGCATAAATACGCTGACCGGAACGCACCGGGGTATCTATTAAACGCGTTTTTGTGACCGGCGTTGTATTTTGCGCTGGAGCCTGCGGTGTGGGAGCTGGACGTGGCGCTTTTTCCTTTCCTTCCGTCAGGATAGGCAGCCCCATCTTTTCAATTTCGGCTTTAAGTTG  >  NC_000913/1225562‑1225828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: