Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	251,799	A→G	E301G (GAG→GGG)	dinB →	DNA polymerase IV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	251,799	0	A	G	100.0%	128.8 / NA	35	E301G (GAG→GGG)	dinB	DNA polymerase IV
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/13);  total (22/13)

GTCTGAATGTGAAGCGATTATCGAGCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGG  >  NC_000913/251665‑251927                                                                                                                                       |                                                                                                                                 gTCTGAATGTGAAGCGATTATCGAGCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGGGCAc                                                                                                                              >  2:88251/1‑139 (MQ=255)                gATTATCGAGCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGGGCACGTCTGGCCGCGGCTg                                                                                                               >  1:357052/1‑139 (MQ=255)                gATTATCGAGCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGGGCACGTCTGGCCGCGGCTg                                                                                                               >  1:414721/1‑139 (MQ=255)                  ttATCGAGCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGGGCACGTCTGGCCGCGGCTGaa                                                                                                             >  1:490002/1‑139 (MQ=255)                       gAGCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGGGCACGTCTGGCCGCGGCTGAATAAAg                                                                                                        <  2:490002/139‑1 (MQ=255)                       gAGCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGGGCACGTCTGGCCGCGGCTGAATAAAg                                                                                                        <  2:122210/139‑1 (MQ=255)                          cGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGGGCACGTCTGGCCGCGGCTGAATAAAGCTg                                                                                                     >  1:614664/1‑139 (MQ=255)                           ggCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGGGCACGTCTGGCCGCGGCTGAATAAAGCTGa                                                                                                    >  1:677614/1‑139 (MQ=255)                                 aTCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGGGCACGTCTGGCCGCGGCTGAATAAAGCTGATCTAAt                                                                                              <  2:677614/139‑1 (MQ=255)                                              aCGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGGGCACGTCTGGCCGCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGt                                                                                 <  2:614664/139‑1 (MQ=255)                                                      tGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGGGCACGTCTGGCCGCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTg                                                                         <  1:275493/139‑1 (MQ=255)                                                       ggCAAAGGTAAAACCTGATTTACTGATTGCTCGCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGGGc                                                                                                                                >  2:37988/1‑83 (MQ=255)                                                              gTAAAACCTGATTTACTGATTGCTCGCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGGGCACGTCTGGCCGCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAAc                                                                 >  2:642913/1‑139 (MQ=255)                                                                        aTTTACTGATTGCTCGCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGGGCACGTCTGGCCGCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGcggcgg                                                       >  2:760357/1‑139 (MQ=255)                                                                        aTTTACTGATTGCTCGCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGGGCACGTCTGGCCGCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGcggcgg                                                       >  2:640582/1‑139 (MQ=255)                                                                           tACTGATTGCTCGCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGGGCACGTCTGGCCGCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCggcgggcg                                                    <  1:640582/139‑1 (MQ=255)                                                                                aTTTCTCGCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGGGCACGTCTGGCCGCGCCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCggcgggcg                                                    <  1:465748/134‑1 (MQ=255)                                                                                aTTGCTCGCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGGGCACGTCTGGCCGCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCggcgggcg                                                    >  2:465748/1‑134 (MQ=255)                                                                                    cTCGCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGGGCACGTCTGGCCGCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCg                                           >  2:509210/1‑139 (MQ=255)                                                                                       gCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGGGCACGTCTGGCCGCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCt                                        >  2:317556/1‑139 (MQ=255)                                                                                        ccAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGGGCACGTCTGGCCGCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCggcggg                                                      >  2:658984/1‑124 (MQ=255)                                                                                        ccAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGGGCACGTCTGGCCGCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCggcggg                                                      <  1:658984/124‑1 (MQ=255)                                                                                            ggggTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGGGCACGTCTGGCCGCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCtggtgg                                   >  1:528359/1‑139 (MQ=255)                                                                                                     ttAAAGTTCGACGATTTTCAGCAAACCACCCAGGGGCACGTCTGGCCGCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCAtg                          <  1:317556/139‑1 (MQ=255)                                                                                                        aaGTTCGACGATTTTCAGCAAACCACCCAGGGGCACGTCTGGCCGCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGa                       >  2:171997/1‑139 (MQ=255)                                                                                                             cgacgaTTTTCAGCAAACCACCCAGGGGCACGTCTGGCCGCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTg                  >  2:527920/1‑139 (MQ=255)                                                                                                              gacgaTTTTCAGCAAACCACCCAGGGGCACGTCTGGCCGCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGgcgc                                                   <  2:418201/105‑1 (MQ=255)                                                                                                              gacgaTTTTCAGCAAACCACCCAGGGGCACGTCTGGCCGCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGgcgc                                                   >  1:418201/1‑105 (MQ=255)                                                                                                              gacgaTTTTCAGCAAACCACCCAGGGGCACGTCTGGCCGCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCt                               >  2:112117/1‑125 (MQ=255)                                                                                                              gacgaTTTTCAGCAAACCACCCAGGGGCACGTCTGGCCGCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCt                               <  1:112117/125‑1 (MQ=255)                                                                                                               acgaTTTTCAGCAAACCACCCAGGGGCACGTCTGGCCGCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCt                >  2:692074/1‑139 (MQ=255)                                                                                                                        aGCAAACCACCCAGGGGCACGTCTGGCCGCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCa       <  1:527920/139‑1 (MQ=255)                                                                                                                             accaccCAGGGGCACGTCTGGCCGCGGCTGAATAAAg                                                                                                        >  1:557005/1‑37 (MQ=255)                                                                                                                             accaccCAGGGGCACGTCTGGCCGCGGCTGAATAAAg                                                                                                        <  2:557005/37‑1 (MQ=255)                                                                                                                             accaccCAGGGGCACGTCTGGCCGCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATgg  >  2:741416/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                 GTCTGAATGTGAAGCGATTATCGAGCGGCTGTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCTCGCCAGGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACCCAGGAGCACGTCTGGCCGCGGCTGAATAAAGCTGATCTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGGGCGCGGTGTGCGTCTGGTGGGGCTGCATGTGACGTTGCTTGACCCGCAAATGG  >  NC_000913/251665‑251927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑