Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,458,617 A→G A118A (GCA→GCG paaG → 1,2‑epoxyphenylacetyl‑CoA isomerase, oxepin‑CoA‑forming

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,458,6170AG100.0% 92.2 / NA 25A118A (GCA→GCGpaaG1,2‑epoxyphenylacetyl‑CoA isomerase, oxepin‑CoA‑forming
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/12);  total (13/12)

CCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCACTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAGCGCGCGCCATGGGGCTG  >  NC_000913/1458490‑1458752
                                                                                                                               |                                                                                                                                       
ccGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGa                                                                                                                              <  2:574444/139‑1 (MQ=255)
                                        cccACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCg                                                                                      <  1:635956/139‑1 (MQ=255)
                                         ccACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGt                                                                                     >  2:582557/1‑139 (MQ=255)
                                          cACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGAc                                                                                                                             >  1:453269/1‑98 (MQ=255)
                                          cACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGAc                                                                                                                             <  2:453269/98‑1 (MQ=255)
                                          cACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGtt                                                                                    >  1:561888/1‑139 (MQ=255)
                                            cTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTa                                                                                  <  1:779521/139‑1 (MQ=255)
                                             tGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATgg                                                                                        >  2:777600/1‑132 (MQ=255)
                                             tGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATgg                                                                                        <  1:777600/132‑1 (MQ=255)
                                              ggTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGt                                                                                >  1:63578/1‑139 (MQ=255)
                                                     cGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGAc                                                                                                                             >  1:568629/1‑87 (MQ=255)
                                                     cGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGAc                                                                                                                             <  2:568629/87‑1 (MQ=255)
                                                       ccTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGc                                                                       >  1:245289/1‑139 (MQ=255)
                                                                         aCCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGACGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGt                                                     <  2:561888/139‑1 (MQ=255)
                                                                                   tgtgCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGt                                           >  1:567755/1‑139 (MQ=255)
                                                                                            aaTGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGAtt                                                          <  1:526318/115‑1 (MQ=255)
                                                                                            aaTGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGAtt                                                          >  2:526318/1‑115 (MQ=255)
                                                                                               ggcgtggcgGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCAcgcg                               >  1:658612/1‑139 (MQ=255)
                                                                                                    ggcggcAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGcc                          >  1:654633/1‑139 (MQ=255)
                                                                                                           ggggcaggCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAg                   >  2:235148/1‑139 (MQ=255)
                                                                                                               caggCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAgcgcg               <  2:658612/139‑1 (MQ=255)
                                                                                                               caggCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAgcgcg               <  1:235148/139‑1 (MQ=255)
                                                                                                                   cgcAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAGCGCGCGcc           <  2:567755/139‑1 (MQ=255)
                                                                                                                       acacTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAgcgc                >  1:725625/1‑130 (MQ=255)
                                                                                                                       acacTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAgcgc                <  2:725625/130‑1 (MQ=255)
                                                                                                                            ggcgctggGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAGCGCGCGCCATGGGGCTg  >  2:726051/1‑139 (MQ=255)
                                                                                                                               |                                                                                                                                       
CCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCACTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAGCGCGCGCCATGGGGCTG  >  NC_000913/1458490‑1458752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: